LOCUS       sequence-193265-        8975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a chimeric KLF4-activator fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.07"; journalName: "Cell Rep"; date: 
            "2017-08-15- 15"; volume: "20"; issue: "7"; pages: "1585-1596"
COMMENT     Sequence Label: pSN30
FEATURES             Location/Qualifiers
     source          1..8975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             526..1233
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(671..689)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     939..958
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1243..1299
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2716..2826
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     misc_feature    2842..3430
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2895..2915)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3313..3324)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3583..3763
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3835..3956
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3872..3891)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3926..3945
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3996..4131
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4151..4170)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4152..4170)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4180..4197)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4180..4196)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4189..4211)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4338..4793
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4425..4444)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4635..4656
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4819..4923
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4924..4992,4993..5784)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5142..5161)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5955..6543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6444..6463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6697..6714
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6831..6852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6867..6872,6873..6890,6891..6897)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6905..6921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6910..6932
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6929..6945
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6929..6945
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6964..6984
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6966..6984
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7012..7238
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7239..7419
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7466..7591
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8084..8317
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8502..8546
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8813..8930
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      601 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      661 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      721 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      781 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      841 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      901 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      961 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     1021 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     1081 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     1141 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     1201 cactccaccg gcggcatgga cgagctgtac aagggatctg gagcaacaaa cttctcacta
     1261 ctcaaacaag caggtgacgt ggaggagaat cccggcccta tggctgtcag cgacgcgctg
     1321 ctcccatctt tctccacgtt cgcgtctggc ccggcgggaa gggagaagac actgcgtcaa
     1381 gcaggtgccc cgaataaccg ctggcgggag gagctctccc acatgaagcg acttccccca
     1441 gtgcttcccg gccgccccta tgacctggcg gcggcgaccg tggccacaga cctggagagc
     1501 ggcggagccg gtgcggcttg cggcggtagc aacctggcgc ccctacctcg gagagagacc
     1561 gaggagttca acgatctcct ggacctggac tttattctct ccaattcgct gacccatcct
     1621 ccggagtcag tggccgccac cgtgtcctcg tcagcgtcag cctcctcttc gtcgtcgccg
     1681 tcgagcagcg gccctgccag cgcgccctcc acctgcagct tcacctatcc gatccgggcc
     1741 gggaacgacc cgggcgtggc gccgggcggc acgggcggag gcctcctcta tggcagggag
     1801 tccgctcccc ctccgacggc tcccttcaac ctggcggaca tcaacgacgt gagcccctcg
     1861 ggcggcttcg tggccgagct cctgcggcca gaattggacc cggtgtacat tccgccgcag
     1921 cagccgcagc cgccaggtgg cgggctgatg ggcaagttcg tgctgaaggc gtcgctgagc
     1981 gcccctggca gcgagtacgg cagcccgtcg gtcatcagcg tcagcaaagg cagccctgac
     2041 ggcagccacc cggtggtggt ggcgccctac aacggcgggc cgccgcgcac gtgccccaag
     2101 atcaagcagg aggcggtctc ttcgtgcacc cacttgggcg ctggaccccc tctcagcaat
     2161 ggccaccggc cggctgcaca cgacttcccc ctggggcggc agctccccag caggactacc
     2221 ccgaccctgg gtcttgagga agtgctgagc agcagggact gtcaccctgc cctgccgctt
     2281 cctcccggct tccatcccca cccggggccc aattacccat ccttcctgcc cgatcagatg
     2341 cagccgcaag tcccgccgct ccattaccaa gagctcatgc cacccggttc ctgcatgcca
     2401 gaggagccca agccaaagag gggaagacga tcgtggcccc ggaaaaggac cgccacccac
     2461 acttgtgatt acgcgggctg cggcaaaacc tacacaaaga gttcccatct caaggcacac
     2521 ctgcgaaccc acacaggtga gaaaccttac cactgtgact gggacggctg tggatggaaa
     2581 ttcgcccgct cagatgaact gaccaggcac taccgtaaac acacggggca ccgcccgttc
     2641 cagtgccaaa aatgcgaccg agcattttcc aggtcggacc acctcgcctt acacatgaag
     2701 aggcattttc cggccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     2761 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     2821 atgctccccg ggtaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     2881 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2941 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3001 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3061 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3121 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3181 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     3241 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3301 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3361 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3421 gcctccccgc ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg
     3481 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3541 gaagacaaga tatcctgcta gctccggatc cggacctgta ctgggtctct ctggttagac
     3601 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     3661 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     3721 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     3781 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     3841 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3901 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3961 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4021 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4081 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     4141 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     4201 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     4261 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4321 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4381 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4441 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4501 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4561 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4621 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4681 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4741 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     4801 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4861 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4921 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4981 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5041 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5101 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5161 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5221 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5281 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5341 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5401 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5461 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5521 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5581 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5641 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5701 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5761 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5821 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5881 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6061 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6601 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6661 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6721 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6781 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     6841 tagctcactc attaggcacc ccaggtttta cactttatgc ttccggctcg tatgttgtgt
     6901 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     6961 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     7021 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     7081 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     7141 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     7201 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     7261 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7321 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7381 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     7441 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     7501 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     7561 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     7621 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     7681 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     7741 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     7801 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     7861 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     7921 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     7981 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     8041 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     8101 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     8161 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     8221 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     8281 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     8341 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     8401 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     8461 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     8521 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     8581 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     8641 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     8701 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     8761 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     8821 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     8881 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg
     8941 agactagcct cgagaagctt gatatcgaat tccac
//
