LOCUS       sequence-193268-        9422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a chimeric KLF4-repressor fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 9422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.07"; journalName: "Cell Rep"; date: 
            "2017-08-15- 15"; volume: "20"; issue: "7"; pages: "1585-1596"
COMMENT     Sequence Label: pSN33
FEATURES             Location/Qualifiers
     source          1..9422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             526..1233
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(671..689)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     939..958
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1243..1299
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    3289..3877
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3342..3362)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3760..3771)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4030..4210
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4282..4403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4319..4338)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4373..4392
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4443..4578
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4598..4617)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4599..4617)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4627..4644)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4627..4643)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4636..4658)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4785..5240
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4872..4891)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5082..5103
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5266..5370
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5371..5439,5440..6231)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5589..5608)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6402..6990
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6891..6910
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7144..7161
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7278..7299
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7314..7319,7320..7337,7338..7344)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7352..7368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7357..7379
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7376..7392
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7376..7392
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7411..7431
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7413..7431
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7459..7685
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7686..7866
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7913..8038
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8531..8764
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8949..8993
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9260..9377
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      601 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      661 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      721 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      781 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      841 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      901 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      961 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     1021 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     1081 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     1141 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     1201 cactccaccg gcggcatgga cgagctgtac aagggatctg gagcaacaaa cttctcacta
     1261 ctcaaacaag caggtgacgt ggaggagaat cccggcccta tggctgtcag cgacgcgctg
     1321 ctcccatctt tctccacgtt cgcgtctggc ccggcgggaa gggagaagac actgcgtcaa
     1381 gcaggtgccc cgaataaccg ctggcgggag gagctctccc acatgaagcg acttccccca
     1441 gtgcttcccg gccgccccta tgacctggcg gcggcgaccg tggccacaga cctggagagc
     1501 ggcggagccg gtgcggcttg cggcggtagc aacctggcgc ccctacctcg gagagagacc
     1561 gaggagttca acgatctcct ggacctggac tttattctct ccaattcgct gacccatcct
     1621 ccggagtcag tggccgccac cgtgtcctcg tcagcgtcag cctcctcttc gtcgtcgccg
     1681 tcgagcagcg gccctgccag cgcgccctcc acctgcagct tcacctatcc gatccgggcc
     1741 gggaacgacc cgggcgtggc gccgggcggc acgggcggag gcctcctcta tggcagggag
     1801 tccgctcccc ctccgacggc tcccttcaac ctggcggaca tcaacgacgt gagcccctcg
     1861 ggcggcttcg tggccgagct cctgcggcca gaattggacc cggtgtacat tccgccgcag
     1921 cagccgcagc cgccaggtgg cgggctgatg ggcaagttcg tgctgaaggc gtcgctgagc
     1981 gcccctggca gcgagtacgg cagcccgtcg gtcatcagcg tcagcaaagg cagccctgac
     2041 ggcagccacc cggtggtggt ggcgccctac aacggcgggc cgccgcgcac gtgccccaag
     2101 atcaagcagg aggcggtctc ttcgtgcacc cacttgggcg ctggaccccc tctcagcaat
     2161 ggccaccggc cggctgcaca cgacttcccc ctggggcggc agctccccag caggactacc
     2221 ccgaccctgg gtcttgagga agtgctgagc agcagggact gtcaccctgc cctgccgctt
     2281 cctcccggct tccatcccca cccggggccc aattacccat ccttcctgcc cgatcagatg
     2341 cagccgcaag tcccgccgct ccattaccaa gagctcatgc cacccggttc ctgcatgcca
     2401 gaggagccca agccaaagag gggaagacga tcgtggcccc ggaaaaggac cgccacccac
     2461 acttgtgatt acgcgggctg cggcaaaacc tacacaaaga gttcccatct caaggcacac
     2521 ctgcgaaccc acacaggtga gaaaccttac cactgtgact gggacggctg tggatggaaa
     2581 ttcgcccgct cagatgaact gaccaggcac taccgtaaac acacggggca ccgcccgttc
     2641 cagtgccaaa aatgcgaccg agcattttcc aggtcggacc acctcgcctt acacatgaag
     2701 aggcattttg gaaagaaaac caagcggaca gctgacagtt cttcttcaga ggatgaggag
     2761 gagtatgtcg tggagaaggt gctagacagg cgcgtggtta agggacaagt ggaatatcta
     2821 ctgaagtgga aaggcttttc tgaggagcac aatacttggg aacctgagaa aaacttggat
     2881 tgccctgagc taatttctga atttatgaaa aagtataaga agatgaagga gggtgaaaat
     2941 aataaaccca gggagaagtc agaaagtaac aagaggaaat ccaatttctc aaacagtgcc
     3001 gatgacatca aatctaaaaa aaagagagag cagagcaatg atatcgctcg gggctttgag
     3061 agaggactgg aaccagaaaa gatcattggg gcaacagatt cctgtggtga tttaatgttc
     3121 ctaatgaaat ggaaagacac agatgaagct gacctggttc ttgcaaaaga agctaatgtg
     3181 aaatgtccac aaattgtgat agcattttat gaagagagac tgacatggca tgcatatcct
     3241 gaggatgcgg aaaacaaaga gaaagaaaca gcaaagagct aagtcgacaa tcaacctctg
     3301 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3361 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3421 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3481 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3541 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3601 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3661 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     3721 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3781 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3841 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt
     3901 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     3961 gactggaagg gctaattcac tcccaacgaa gacaagatat cctgctagct ccggatccgg
     4021 acctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4081 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4141 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4201 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     4261 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     4321 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4381 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     4441 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4501 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4561 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     4621 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     4681 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     4741 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     4801 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4861 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4921 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4981 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5041 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5101 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5161 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5221 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5281 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5341 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5401 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5461 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5521 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5581 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5641 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5701 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5761 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5821 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5881 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5941 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6001 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6061 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6121 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6181 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6241 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6301 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6361 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6421 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6481 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6541 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6601 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6661 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6721 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6781 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6841 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6901 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6961 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7021 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7081 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7141 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7201 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7261 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggttttacac
     7321 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7381 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     7441 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     7501 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     7561 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     7621 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     7681 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7741 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7801 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7861 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     7921 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     7981 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     8041 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     8101 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     8161 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     8221 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     8281 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     8341 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     8401 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     8461 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     8521 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     8581 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     8641 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     8701 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     8761 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     8821 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     8881 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     8941 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     9001 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     9061 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     9121 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     9181 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     9241 ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg
     9301 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     9361 caaaaattca aaattttatc gatcacgaga ctagcctcga gaagcttgat atcgaattcc
     9421 ac
//
