LOCUS       sequence-193272-        6920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term of HCF-1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Wilson AC, Freiman RN, Goto H, Nishimoto T, Herr W
  TITLE     VP16 targets an amino-terminal domain of HCF involved in cell cycle 
            progression.
  JOURNAL   Mol Cell Biol. 1997 Oct;17(10):6139-46.
  PUBMED    9315674
REFERENCE   2  (bases 1 to 6920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1997-10"; volume: "17(10)"; pages: "6139-46"
COMMENT     Sequence Label: pCGN-HCF-1-C600
FEATURES             Location/Qualifiers
     source          1..6920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     458..478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     504..528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             666..692
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             672..698
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          2618..3190
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3322..3341)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3387..3442
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3441..3460)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3796..3931
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4037..4054)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4208..4796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4288..4307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4967..5758,5759..5827))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5590..5609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5828..5932)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6000..6018
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6056..6078)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6178..6197
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6264..6719)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6401..6422)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6613..6632
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6845..6867
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6859..6876
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6860..6876
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6883..6902
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6883..6901
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(6920,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt
      661 ctagctatcc ttatgacgtg cctgactatg ccagcctggg aggaccttct agaaccagta
      721 gatctgtcac ttccaacatg agttcaaacc aagacccccc acctgctgcc agcgatcagg
      781 gagaggtgga gagcacccag ggcgacagcg tgaacatcac cagctccagt gccatcacga
      841 caaccgtgtc ctccacactg acgcgggctg tgaccaccgt gacgcagtcc acaccggtcc
      901 cgggcccctc tgtgccgccc ccagaggaac tccaggtgtc gccaggtcct cgccagcagc
      961 tgccgccacg gcagcttctg cagtcggctt ccacagccct gatgggggag tccgccgagg
     1021 tcctgtcagc ctcccagacc cctgagctcc cggccgccgt ggatctgagc agcacagggg
     1081 agccatcttc gggccaggag tctgccggct ctgcggtggt ggccactgtg gtggtccagc
     1141 cacccccacc cacacagtcc gaagtagacc agttatcact tccccaagag ctaatggccg
     1201 aggcccaagc tggcaccacc accctcatgg taacggggct cacccccgag gagctggcag
     1261 tgacggctgc tgcagaagca gctgcccagg ccgcagccac ggaggaagcc caggccctgg
     1321 ccatccaggc ggtgctccag gccgcgcagc aggccgtcat gggcaccggc gagcccatgg
     1381 acacctccga ggcagcagca accgtgactc aggcggagct ggggcacctg tcggccgagg
     1441 gtcaggaggg ccaggccacc accataccca ttgtgctgac acagcaggag ctggctgccc
     1501 tggtgcagca gcagcagctg caggaggccc aggcccagca gcagcatcac cacctcccca
     1561 ctgaggccct ggcccctgcc gacagtctca acgacccagc cattgagagc aattgcctca
     1621 atgagctggc cggcacggtc cccagcactg tggcgctgct gccctcaacg gccactgaga
     1681 gcctggctcc atccaacaca tttgtggccc cccagccggt tgtggtggcc agcccagcca
     1741 agctgcaggc tgcagctacc ctgaccgaag tggccaatgg catcgagtcc ctgggtgtga
     1801 agccagacct cccgccccca cccagcaaag cccccatgaa gaaggaaaac cagtggtttg
     1861 atgtgggagt cattaagggc accaatgtaa tggtgacaca ctatttcctg ccaccagatg
     1921 atgctgtccc atcagacgat gatttgggca ccgtccctga ctataaccag ctgaagaagc
     1981 aggagctgca gccaggcaca gcctataagt ttcgtgttgc cggaatcaat gcctgtggcc
     2041 gggggccctt cagcgaaatc tcagccttta agacgtgcct gcctggtttc ccaggggccc
     2101 cttgtgccat taaaatcagc aaaagtccgg atggtgctca cctcacctgg gagccaccct
     2161 ctgtgacctc cggcaagatt atcgagtact ccgtgtacct ggccatccag agctcacagg
     2221 ctgggggcga gctcaagagc tccaccccgg cccagctggc cttcatgcgg gtgtactgtg
     2281 ggcccagccc ctcctgcctg gtgcagtcct ccagcctttc caacgcccac atcgactaca
     2341 ccaccaagcc cgccatcatc ttccgcatcg ccgcccgcaa tgagaagggc tatggcccgg
     2401 ccacacaagt gaggtggctg caggaaacca gtaaagacag ctctggcacc aagccagcca
     2461 acaagcggcc catgtcctct ccagaaatga aatctgctcc aaagaaatct aaggccgatg
     2521 gtcagggcga gcagaagctg atctctgagg aggacacggt atcgcgataa gcttgatatc
     2581 gaattcctgc agcccggggg atcctgagaa cttcagggtg agtttgggga cccttgattg
     2641 ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt
     2701 gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt
     2761 ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt
     2821 aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta
     2881 tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt
     2941 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
     3001 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacac
     3061 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
     3121 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctc
     3181 tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga
     3241 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     3301 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     3361 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     3421 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     3481 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     3541 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     3601 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     3661 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     3721 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgaggagct
     3781 ttttgcaaaa gcctaggcct ccaaaaaagc ctcttcacta cttctggaat agctcagagg
     3841 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     3901 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     3961 ctgactaatt gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4021 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4081 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4141 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4201 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4261 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4321 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4381 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4441 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4501 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4561 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4621 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4681 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4741 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4801 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4861 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4921 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4981 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5041 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5101 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5161 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5221 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5281 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5341 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5401 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5461 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5521 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5581 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5641 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5701 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5761 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5821 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5881 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5941 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     6001 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     6061 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     6121 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     6181 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     6241 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     6301 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     6361 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     6421 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     6481 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     6541 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     6601 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     6661 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     6721 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     6781 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     6841 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
     6901 ggcgaattcg agctcgcccc
//
