LOCUS       sequence-193276-        9892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of MBP-tagged clathrin heavy chain large 
            fragment including N-terminal domain, his tagged at C-terminus for 
            purification..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ
  TITLE     New tools for ""hot-wiring"" clathrin-mediated endocytosis with 
            temporal and spatial precision.
  JOURNAL   J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 
            10.1083/jcb.201702188.
  PUBMED    28954824
REFERENCE   2  (bases 1 to 9892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188."
COMMENT     Sequence Label: MBP-CHC(1-1074)-His6
FEATURES             Location/Qualifiers
     source          1..9892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..87
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             88..1170
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(107..126)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1413..1418,1419..1434,1435..1441)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1449..1465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1454..1476
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1535..2635
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2609..2632
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2684..2707
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     RBS             5799..5807
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5960..5977
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5990..6007)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5990..6006)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5999..6021)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6185..6202)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      6355..6441
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6533..6560
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6579..6670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6671..6739,6740..7531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6889..6908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7573..8086)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7768..7789)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7980..7999
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      8197..8785
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8686..8705
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8939..8956
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8971..9113
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9039..9058)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9199..9221
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9215..9406)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 cgaaattccg acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg
       61 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
      121 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      181 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      241 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      301 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      361 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      421 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      481 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      541 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      601 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      661 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      721 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      781 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      841 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcagtagtg
      901 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      961 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
     1021 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1081 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1141 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac
     1201 tcattaggca caattctcat gtttgacagc ttatcatcga ctgcacggtg caccaatgct
     1261 tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgca
     1321 taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat
     1381 aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg ctcgtataat
     1441 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agccagtccg tttaggtgtt
     1501 ttcacgagca cttcaccaac aaggaccata gcatatgaaa atcgaagaag gtaaactggt
     1561 aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta agaaattcga
     1621 gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag agaaattccc
     1681 acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg accgctttgg
     1741 tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt tccaggacaa
     1801 gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat
     1861 cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac
     1921 ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga gcgcgctgat
     1981 gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg ggggttatgc
     2041 gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata acgctggcgc
     2101 gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga atgcagacac
     2161 cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg
     2221 cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa cggtactgcc
     2281 gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc
     2341 cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa aactatctgc tgactgatga
     2401 aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga agtcttacga
     2461 ggaagagttg gcgaaagatc cacgtattgc cgccactatg gaaaacgccc agaaaggtga
     2521 aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta ctgcggtgat
     2581 caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc agactaattc
     2641 gagctcgaac aacaacaaca ataacaataa caacaacctc gggctggaag ttctgttcca
     2701 ggggcccgaa ttcgggatct gcatggccca gattctgcca attcgttttc aggagcatct
     2761 ccagctccag aacctgggta tcaacccagc aaacattggc ttcagtaccc tgactatgga
     2821 gtctgacaaa ttcatctgca ttagagaaaa agtaggagag caggcccagg tggtaatcat
     2881 tgatatgaat gacccaagta atccaattcg aagaccaatt tcagcagaca gcgccatcat
     2941 gaatccagct agcaaagtaa ttgcactgaa agctgggaaa actcttcaga tttttaacat
     3001 tgaaatgaaa agtaaaatga aggctcatac catgactgat gatgtcacct tttggaaatg
     3061 gatctctttg aatacggttg ctcttgttac ggataatgca gtttatcact ggagtatgga
     3121 aggagagtct cagccagtga aaatgtttga tcgccattct agccttgcag ggtgccagat
     3181 tatcaattac cgtacagatg caaaacaaaa gtggttactt ctgactggta tatctgcaca
     3241 gcaaaatcgt gtggtgggag ctatgcagct atattctgta gataggaaag tgtctcagcc
     3301 cattgaagga catgcagcta gctttgcaca gtttaagatg gaaggaaatg cagaagaatc
     3361 aacgttattt tgttttgcag ttcggggcca agctggaggg aagttacata ttattgaagt
     3421 tggcacacca cctacaggga accagccctt tccaaagaag gcagtggatg tcttctttcc
     3481 tccagaagca caaaatgatt ttcctgttgc aatgcagatc agtgaaaagc atgatgtggt
     3541 gttcttgata accaagtatg gttatatcca cctctatgat cttgagactg gtacctgcat
     3601 ctacatgaat agaatcagtg gagaaacaat ttttgttact gcacctcatg aagccacagc
     3661 tggaataatt ggagtaaaca gaaagggaca agttctgtca gtgtgtgtgg aagaagaaaa
     3721 cataattcct tacatcacca atgttctaca aaatcctgat ttggctctga gaatggctgt
     3781 acgtaataac ttagccggtg ctgaagaact ctttgcccgg aaatttaatg ctctttttgc
     3841 ccagggaaat tactcggagg cagcaaaggt ggctgctaat gcaccaaagg gaattcttcg
     3901 tactccagac actatccgtc ggttccagag tgtcccagcc cagccaggtc aaacttctcc
     3961 tctacttcag tactttggta tccttttgga ccagggacag ctcaacaaat acgaatcctt
     4021 agagctttgt aggcctgtac ttcagcaagg gcgaaaacag cttttggaga aatggttaaa
     4081 agaagataag ctggaatgtt ctgaagaact gggtgatctt gtgaaatctg tggaccctac
     4141 attggcactt agtgtgtacc taagggctaa cgtcccaaat aaagtcattc agtgctttgc
     4201 agaaacaggt caagtccaaa agattgtttt atatgctaaa aaagttggat acactccaga
     4261 ttggatattt ctgctgagaa atgtaatgcg aatcagtcca gatcagggac agcagtttgc
     4321 ccaaatgtta gttcaagatg aagagcctct tgctgacatc acacagattg tagatgtctt
     4381 tatggaatac aatctaattc agcagtgtac tgcattcttg cttgatgctc tgaagaataa
     4441 tcgcccatct gaaggtcctt tacagacgcg gttacttgag atgaacctta tgcatgcgcc
     4501 tcaagttgca gatgctattc taggcaatca gatgttcaca cattatgacc gggctcatat
     4561 tgctcaactg tgtgaaaagg ctggcctact gcagcgtgca ttagaacatt tcactgattt
     4621 atatgatata aaacgtgcag tggttcacac ccatcttctt aaccctgagt ggttagtcaa
     4681 ctactttggt tccttatcag tagaagactc cctagaatgt ctcagagcca tgctgtctgc
     4741 caacatccgt cagaatctgc agatttgtgt tcaggtggct tctaaatatc atgaacaact
     4801 gtcaactcag tctctgattg aactttttga atctttcaag agttttgaag gtctctttta
     4861 ttttctggga tccattgtta actttagcca ggacccagat gtgcacttta aatatattca
     4921 ggcagcttgc aagactgggc aaatcaaaga agtagaaaga atctgtagag aaagcaactg
     4981 ctacgatcct gagcgagtca agaattttct taaggaagca aaactaacag atcagctacc
     5041 acttatcatt gtgtgtgatc gatttgactt tgtccatgat ttggtgctct atttatatag
     5101 aaataatctt caaaagtata tagagatata tgtacagaag gtgaatccaa gtcgacttcc
     5161 tgtagttatt ggaggattac ttgatgttga ctgttctgaa gatgtcataa aaaacttgat
     5221 tcttgttgta agaggtcaat tctctactga tgagcttgtt gctgaggttg aaaaaagaaa
     5281 cagattgaaa ctgcttctgc cttggctaga ggccagaatt catgagggct gtgaggagcc
     5341 tgctactcac aatgccttag ccaaaatcta catagacagt aataacaacc cggagagatt
     5401 tcttcgtgaa aatccctact atgacagtcg cgttgttgga aagtattgtg agaagagaga
     5461 tccacatctg gcctgtgttg cttatgaacg tggccaatgt gatctggaac ttattaatgt
     5521 ttgcaatgag aattccctct tcaaaagtct ttctcgctac ctggtacgtc gaaaggatcc
     5581 agaattgtgg ggcagcgtgc tgctggaaag caatccttac aggagacccc taattgacca
     5641 ggttgtacaa acagctttgt ctgagactca ggaccctgaa gaagtgtcag taactgtaaa
     5701 ggctttcatg actgcagacc ttcctaatga actcattgaa ctgctggaga aaattgtcct
     5761 tgataactct gtattcagtg aacacaggaa tctgcaaaac ctccttatcc tcactgcaat
     5821 taaggctgac cgtacacgtg ttatggagta tattaaccgc ctggataatt atgatgcccc
     5881 agatattgcc aatatcgcca tcagcaatga gctgtttgaa gaagcatttg ccattttccg
     5941 gaaagcggcc gcagtcgacc accatcacca tcaccattaa aagcttggca ctggccgtcg
     6001 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     6061 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     6121 agttgcgcag cctgaatggc gaatggcagc ttggctgttt tggcggatga gataagattt
     6181 tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg
     6241 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
     6301 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata
     6361 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     6421 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     6481 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc
     6541 atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca
     6601 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6661 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6721 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6781 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6841 ttttcgcccc gaagaacgtt tcccaatgat gagcactttt aaagttctgc tatgtggcgc
     6901 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6961 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7021 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7081 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7141 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7201 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7261 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7321 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7381 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7441 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7501 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7561 ttagattgat ttaccccggt tgataatcag aaaagcccca aaaacaggaa gattgtataa
     7621 gcaaatattt aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     7681 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     7741 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     7801 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     7861 ccatcaccca aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     7921 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     7981 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     8041 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtaaaa ggatctaggt
     8101 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8161 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8221 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8281 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8341 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8401 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8461 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8521 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8581 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8641 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8701 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8761 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8821 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8881 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8941 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     9001 gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca
     9061 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     9121 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     9181 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     9241 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgcagcgat tcacagatgt
     9301 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
     9361 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
     9421 aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga
     9481 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg
     9541 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta
     9601 atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca
     9661 taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga
     9721 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct
     9781 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc
     9841 tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cc
//
