LOCUS       sequence-19328-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf256
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             437..478
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             488..505
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(528..545)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    534..758
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctcggat ccgtaccctt caccgaattc cagctgacca ccatggaagt tatcactgat
       61 cgtctagacg atatagtgaa acaaaatata gcggatgaaa aatttgtaga ttttgttata
      121 cacggtctag agcatcaatg tcctgctata cttcgaccat taattaggtt gtttattgat
      181 atactattat ttgtcatagt aatttatatt tttacggtac gtctagtaag tagaaattat
      241 caaatgttgt tggcgttggt ggcgctagtc atcacattaa ctatttttta ttactttata
      301 ctataatagt actagactga cttctaacaa acatctcacc tggcaattcc cggggatccg
      361 aataaagggt caagacaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga
      421 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
      481 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc
      541 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      601 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      661 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
      721 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
      781 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcatt
//
