LOCUS       sequence-193280-        7900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term of HCF-1 protein without amino acids 382-450.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Wilson AC, Freiman RN, Goto H, Nishimoto T, Herr W
  TITLE     VP16 targets an amino-terminal domain of HCF involved in cell cycle 
            progression.
  JOURNAL   Mol Cell Biol. 1997 Oct;17(10):6139-46.
  PUBMED    9315674
REFERENCE   2  (bases 1 to 7900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1997-10"; volume: "17(10)"; pages: "6139-46"
COMMENT     Sequence Label: pCGN-HCF-1-N1011-Delta-382-450
FEATURES             Location/Qualifiers
     source          1..7900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     458..478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     504..528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             666..692
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             672..698
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             2921..2938
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          3598..4170
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(4302..4321)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4367..4422
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4421..4440)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      4776..4911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5017..5034)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5188..5776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5268..5287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5947..6738,6739..6807))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6570..6589
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6808..6912)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6980..6998
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7036..7058)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7158..7177
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7244..7699)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7381..7402)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7593..7612
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7825..7847
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7839..7856
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7840..7856
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7863..7882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7863..7881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(7900,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt
      661 ctagctatcc ttatgacgtg cctgactatg ccagcctggg aggaccttct agatcacgtc
      721 gtgcatctgg tacgtacgga cgcgtcgctt cggccgtgtc gcccgccaac ttgccagcgg
      781 tgcttctgca gccccgctgg aagcgagtgg tgggctggtc gggtccggtg ccacggcccc
      841 gccacggcca ccgcgccgtg gccatcaagg agctcatcgt ggtgtttggc ggcggcaacg
      901 agggaatagt ggacgaactg cacgtgtaca acacggcaac caaccagtgg ttcatcccag
      961 ccgtgagggg ggacattccc cctgggtgtg cagcctatgg cttcgtgtgt gacgggactc
     1021 gcctcctggt gtttggtggg atggtggagt atgggaaata cagcaatgac ctctacgaac
     1081 tccaggcgag ccggtgggag tggaagagac tcaaagcaaa gacgcccaaa aacgggcccc
     1141 ctccgtgtcc tcgactcggg cacagcttct cccttgtggg caacaaatgc tacctgtttg
     1201 ggggtctggc caatgatagc gaggacccaa agaacaacat tccaaggtac ctgaatgact
     1261 tatatatcct ggaattacgg ccaggctctg gagtggtagc ctgggacatt cccatcactt
     1321 acggggtcct accaccaccc cgggagtcac atactgccgt ggtctacacc gaaaaagaca
     1381 ataagaagtc caagctggtg atctacggcg ggatgagtgg ctgcaggctg ggggacctgt
     1441 ggaccctaga tattgacacc ctgacgtgga ataagcccag tctcagcggg gtggcgcctc
     1501 ttcctcgcag tctccactcg gcaaccacca tcggaaataa aatgtacgtg tttggtggct
     1561 gggtgcctct cgtcatggat gacgtcaaag tggccacaca cgagaaggag tggaagtgta
     1621 ccaacacgct ggcttgtctc aacctggata ccatggcctg ggagaccatc ctgatggata
     1681 cactggagga caacatcccc cgtgctcggg ctggccactg cgcagtcgcc atcaacaccc
     1741 gcctgtacat ttggagtggg cgtgacggct accgcaaggc ctggaacaac caggtctgct
     1801 gcaaggacct ctggtaccta gagacagaaa agccaccacc cccagcccga gtacaactgg
     1861 tacgcgccaa caccaactcc ctggaggctg cccccgcacc cccgaccacc accaccatcc
     1921 aggtcttgcc aacggtgcct ggcagctcca tttctgtgcc caccgcagcc aggactcaag
     1981 gtgtccctgc tgttctcaaa gtgaccggtc ctcaggctac aacaggaact ccattggtca
     2041 ccatgcgacc tgccagccag gctgggaaag cccctgtcac cgtgacctcc cttcccgccg
     2101 gagtgcggat ggttgtgcca acacagagtg cccagggaac ggtgattggc agtagcccac
     2161 agatgagtgg gatggccgca ctggccgctg cggccgctgc cacccagaag atcccccctt
     2221 cctcggcacc cacggtgctg agtgtcccag cgggtaccac catcgtgaag accatggctg
     2281 tgacacctgg cactaccacc ctcccagcca ctgtgaaggt ggcctcctcg ccagtcatgg
     2341 tgagcaaccc tgccactcgc atgctgaaga ctgcagccgc ccaggtgggg acatcggttt
     2401 cctccgccac caacacgtct acccgcccta tcatcacagt gcacaagtca ggcactgtga
     2461 cagtggccca gcaagcccag gtggtgacca cagttgtggg cggggtcacc aagaccatca
     2521 ccctggtgaa gagccccatc tctgtcccag gaggcagtgc tctgatttcc aatctgggca
     2581 aagtgatgtc ggtggtccag accaaaccag ttcagacttc agcagtcaca ggccaggcgt
     2641 ccacgggtcc tgtgactcag atcatccaga ccaaagggcc cctgccagcg ggaacaatcc
     2701 tgaagctggt gacctcagca gatggcaagc ccaccaccat catcactacc acgcaggcca
     2761 gtggggcggg gaccaagccc accatcctgg gcatcagcag cgtctccccc agtaccacca
     2821 agcccggcac gaccaccatc atcaaaacca tccccatgtc ggccatcatc acccaggcgg
     2881 gcgccacggg tgtgaccagc agtcctggca tcaagtcccc catcaccatc atcaccacca
     2941 aggtgatgac ttcaggaact ggagcacctg cgaaaatcat cactgctgtc cccaaaattg
     3001 ccactggcca cgggcagcag ggagtgaccc aggtggtgct taagggggcc ccgggacagc
     3061 caggcaccat cctccgcact gtgcccatgg ggggtgttcg cctggtcaca cccgtcaccg
     3121 tctccgccgt caagccagcc gtcaccacgt tggttgtgaa aggcaccaca ggtgtcacga
     3181 ccctaggcac agtgacaggc accgtctcca ccagccttgc cggggcgggg ggccacagca
     3241 ctagtgcttc cctggccacg cccatcacca ccttgggcac cattgccacc ctctcaagcc
     3301 aggtgatcaa ccccactgcc atcactgtgt cggccgcaca gaccacgctg acagcggcag
     3361 gcgggctcac aaccccaacc atcaccatgc agcccgtgtc ccagcccacc caggtaactc
     3421 tgatcacggc acctagtggg gtggaggccc agcctgtgca tgacctccct gtgtccattc
     3481 tggcctcccc gactacagaa cagcccaccg ccacagttac catcgccgac tcaggccagg
     3541 gtgatgtgca gcctggcact gtcaccttga gatcttgagg atcctgagaa cttcagggtg
     3601 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga
     3661 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga
     3721 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt
     3781 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt
     3841 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt
     3901 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
     3961 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
     4021 attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga
     4081 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
     4141 catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg
     4201 tctcatcatt ttggcaaaga attcactcct caggtgcagg ctgcctatca gaaggtggtg
     4261 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     4321 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     4381 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     4441 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     4501 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     4561 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     4621 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     4681 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     4741 tggagatccc tcgaggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcttcacta
     4801 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     4861 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     4921 taggggcggg actatggttg ctgactaatt gctgcattaa tgaatcggcc aacgcgcggg
     4981 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5041 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5101 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5161 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5221 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5281 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5341 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5401 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5461 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5521 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5581 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5641 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5701 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5761 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5821 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5881 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5941 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6001 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6061 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6121 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6181 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6241 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6301 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6361 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6421 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6481 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6541 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6601 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6661 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6721 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6781 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6841 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6901 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     6961 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     7021 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     7081 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     7141 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     7201 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat
     7261 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     7321 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     7381 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     7441 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     7501 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     7561 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     7621 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     7681 gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     7741 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     7801 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     7861 tgtaatacga ctcactatag ggcgaattcg agctcgcccc
//
