LOCUS       sequence-193293-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses lambda N protein fused to triple Gfp coupled to an NLS 
            sequence under the control of the constitutively active Potef 
            promotor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Pohlmann T, Baumann S, Haag C, Albrecht M, Feldbrugge M
  TITLE     A FYVE zinc finger domain protein specifically links mRNA transport 
            to endosome trafficking.
  JOURNAL   Elife. 2015 May 18;4. doi: 10.7554/eLife.06041.
  PUBMED    25985087
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-05-18"; volume: "4. doi"; pages: " 10.7554/eLife.06041"
COMMENT     Sequence Label: pPotef-LN22*-3xGNLS-cbx
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..30)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(124..145)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     473..492
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     732..753
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cgctttttcg acctttctct tcttttttgg aggcgctttc atatgtatat cgccagctga
       61 aagtgaaaca aaattatttc tcttgtacag ctcgtccatg ccgagagtga tcccggcggc
      121 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
      181 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
      241 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
      301 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
      361 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
      421 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
      481 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
      541 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
      601 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
      661 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
      721 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accacccc
//
