LOCUS       sequence-193294-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses lambda N protein fused to triple Gfp coupled to an NLS 
            sequence under the control of the constitutively active Potef 
            promotor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Pohlmann T, Baumann S, Haag C, Albrecht M, Feldbrugge M
  TITLE     A FYVE zinc finger domain protein specifically links mRNA transport 
            to endosome trafficking.
  JOURNAL   Elife. 2015 May 18;4. doi: 10.7554/eLife.06041.
  PUBMED    25985087
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-05-18"; volume: "4. doi"; pages: " 10.7554/eLife.06041"
COMMENT     Sequence Label: pPotef-LN22*-3xGNLS-cbx
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(46..64,65..87,88..106,107..129,130..148,149..171,
                     172..190,191..213,214..232,233..255,256..274,275..297,
                     298..316)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(353..371,372..394,395..413,414..436,437..455,456..478,
                     479..497,498..520,521..539,540..562,563..581,582..604,
                     605..623)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 tcgaaattcg agctcggtac ccgtaccgag ctcgactttc acttttctct atcactgata
       61 gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac
      121 tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca
      181 ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa
      241 ctcgactttc acttttctct atcactgata gggagtggta aactcgactt tcacttttct
      301 ctatcactga tagggagtgg taaactcgag taccgagctc gactttcact tttctctatc
      361 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
      421 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
      481 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
      541 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
      601 cttttctcta tcactgatag ggagtggtaa actcgagggg atcaattcga ccaatgaggc
      661 gcgagacgag gggacgctgg aagttgaggc gcaagaaaat ttttctctgg ttctgcgcgg
      721 c
//
