LOCUS       sequence-193301-        5930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5930)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NUDT17 (NUDT17A-c014)
FEATURES             Location/Qualifiers
     source          1..5930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             181..351
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             358..378
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    1536..1670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1573..1592)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1627..1646
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1699..1864
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2048..2502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2135..2154)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2344..2365
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2528..2632
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2633..2701,2702..3493)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2851..2870)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3664..4252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4153..4172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4406..4423
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4557..4781
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4848..5381)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5551..5556,5557..5573,5574..5579))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg ggccaccatc atcatcatca ttcttctggt
      181 gtggataaca agttcaacaa ggagcgtcga agagctcgcc gtgaaattcg ccatctgccg
      241 aacctgaacc gcgaacagcg tcgcgcattt attcgcagcc tgcgcgatga tccgagccag
      301 agcgcgaacc tgctggcgga agcgaagaag ctgaacgatg cgcagccgaa gggtacagag
      361 aacctgtact tccaatccat ggctgaagtg cgtgttcaac tgctgctgtc ccgtcgcccg
      421 gaaagcgtgt cgtttgctcg tagcgtttgt ggtctgctgg gtgcgggccc gggtctgggc
      481 acctggccga ttcattgcag cctgaaacgt ggtcgcctgg ttctgagctc tcgtccgttt
      541 ccgggcgcat ctgctcgtct gccgctgcaa cgtccgccgt tttgtccgtt cgcagcactg
      601 gaagaacgtc cgcgtgtccc gggtgcagaa ctgccgaccg atcgtggtgt ggacctgggc
      661 gtggcagtta tcctgcagag ttccgataaa accgtgctgc tgacgcgtcg cgcacgtacg
      721 ctgtcagtct cgccgaacct gtgggtgccg ccgggcggtc atgttgaact ggaagaagaa
      781 ctgctggacg gcggtctgcg cgaactgtgg gaagaatcag gtctgcacct gccgcaaggc
      841 cagttctcgt gggttccgct gggtctgtgg gaaagtgcat atccgccgcg tctgtcctgg
      901 ggcctgccga aatatcatca cattgtgctg tacctgctgg ttatcagcca ggaatctcag
      961 caacagctgc aagcacgcat tcagccgaac ccgaatgaag ttagtgctct gatgtggctg
     1021 accccggatg ttgcagctgc agtcgcagca gctgaagacg gtaccgaaac gccgggtctg
     1081 ctgccgcagg atctgccgcc gagcgtcctg gcagtggaac tggaagaaga cggtcgtgcc
     1141 cgcccgctgg ttctgcatat gagtaccctg ctgcgtatga tcccgacgat ggcagaagat
     1201 aaagaacgcg tctccaccgg tacgaaattt gctctgaaac tgtggctgca acacctgggt
     1261 cgtaccccgc cgccgtgcaa atctgcagca tacctggatc cgggtccggc aaaagaagaa
     1321 tggaacatgg acccgctgcc gccgaatcag ggtagcggca aatgacagta aaggtggata
     1381 cggatccgaa ttcgagctcc gtcgacaagc ttgtcgagaa gtactagagg atcataatca
     1441 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     1501 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     1561 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     1621 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactga
     1681 tatcgcctag gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt
     1741 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc
     1801 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc
     1861 cacagcgggg catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt
     1921 gacaaaccgt catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc
     1981 tcttcgttat taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc
     2041 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     2101 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2161 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     2221 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     2281 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttctta
     2341 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     2401 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     2461 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     2521 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2581 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2641 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2701 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2761 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2821 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2881 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2941 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3001 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3061 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3121 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3181 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3241 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3301 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3361 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3421 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3481 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3541 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3601 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3661 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3721 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3781 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3841 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3901 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3961 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4021 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4081 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4141 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4201 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4261 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4321 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4381 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     4441 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag accagccgcg
     4501 taacctggca aaatcggtta cggttgagta ataaatggat gccctgcgta agcgggtgtg
     4561 ggcggacaat aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta
     4621 aactagacag aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg
     4681 gacttttgtt atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat
     4741 taaagagggg cgtggccaag ggcatggtaa agactatatt cgcggcgttg tgacaattta
     4801 ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta
     4861 cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc
     4921 actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg
     4981 gcctcatgct tgagcagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg
     5041 gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg
     5101 taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc
     5161 ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct
     5221 acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca
     5281 gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg
     5341 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     5401 taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa
     5461 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     5521 ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaac
     5581 tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc
     5641 gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat
     5701 cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc
     5761 tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg
     5821 gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccaggac
     5881 tctagctata gttctagtgg ttggctacgt atactccgga atattaatag
//
