LOCUS       sequence-193304-        5702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5702)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZAK (ZAKA-c057)
FEATURES             Location/Qualifiers
     source          1..5702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    1308..1442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1345..1364)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1399..1418
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1471..1636
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1820..2274
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1907..1926)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2116..2137
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2300..2404
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2405..2473,2474..3265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2623..2642)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3436..4024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3925..3944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4178..4195
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4329..4553
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4620..5153)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5323..5328,5329..5345,5346..5351))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg ggccaccatc atcatcatca ttcttctggt
      181 gtagatctgg gtaccgagaa cctgtacttc caatccatgg gtgcctcctt tgtgcaaatt
      241 aaatttgatg acttgcagtt ttttgaaaac tgcggtggag gaagttttgg gagtgtttat
      301 cgagccaaat ggatatcaca ggacaaggag gtggctgtaa agaagctcct caaaatagag
      361 aaagaggcag aaatactcag tgtcctcagt cacagaaaca tcatccagtt ttatggagta
      421 attcttgaac ctcccaacta tggcattgtc acagaatatg cttctctggg atcactctat
      481 gattacatta acagtaacag aagtgaggag atggatatgg atcacattat gacctgggcc
      541 actgatgtag ccaaaggaat gcattattta catatggagg ctcctgtcaa ggtgattcac
      601 agagacctca agtcaagaaa cgttgttata gctgctgatg gagtattgaa gatctgtgac
      661 tttggtgcct ctcggttcca taaccataca acacacatgt ccttggttgg aactttccca
      721 tggatggctc cagaagttat ccagagtctc cctgtgtcag aaacttgtga cacatattcc
      781 tatggtgtgg ttctctggga gatgctaaca agggaggtcc cctttaaagg tttggaagga
      841 ttacaagtag cttggcttgt agtggaaaaa aacgagagat taaccattcc aagcagttgc
      901 cccagaagtt ttgctgaact gttacatcag tgttgggaag ctgatgccaa gaaacggcca
      961 tcattcaagc aaatcatttc aatcctggag tccatgtcaa atgacacgag ccttcctgac
     1021 aagtgtaact cattcctaca caacaaggcg gagtggaggt gcgaaattga ggcaactctt
     1081 gagaggctaa agaaactaga gcgtgatctc agctttaagg agcaggagct taaatgacag
     1141 taaaggtgga tacggatccg aattcgagct ccgtcgacaa gcttgtcgag aagtactaga
     1201 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     1261 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     1321 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1381 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     1441 tctgatcact gatatcgcct aggagatccg aaccagataa gtgaaatcta gttccaaact
     1501 attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt
     1561 tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact
     1621 attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg
     1681 ccaactccat gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat
     1741 ttttctgtca tctcttcgtt attaatgttt gtaattgact gaatatcaac gcttatttgc
     1801 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     1861 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     1921 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     1981 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2041 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2101 tccacgttct taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     2161 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     2221 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     2281 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2341 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2401 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2461 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2521 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2581 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2641 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2701 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2761 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2821 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2881 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2941 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3001 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3061 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3121 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3181 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3241 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3301 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3361 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3421 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3481 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3541 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3601 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3661 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3721 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3781 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3841 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3901 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3961 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4021 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4081 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4141 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4201 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4261 agaccagccg cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg
     4321 taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac
     4381 aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga
     4441 aaaagcatac tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat
     4501 tgcccgtcgt attaaagagg ggcgtggcca agggcatggt aaagactata ttcgcggcgt
     4561 tgtgacaatt taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt
     4621 taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt
     4681 gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca
     4741 ccaagcgcgt tggcctcatg cttgagcaga ttgatgagcg cggtggcaat gccctgcctc
     4801 cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac
     4861 ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc
     4921 gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg
     4981 gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat
     5041 ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac
     5101 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5161 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     5221 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     5281 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     5341 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     5401 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg
     5461 gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc
     5521 acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg
     5581 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
     5641 ttcgcccagg actctagcta tagttctagt ggttggctac gtatactccg gaatattaat
     5701 ag
//
