LOCUS       sequence-193305-        6404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6404)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PKD2 (PKD2A-c034)
FEATURES             Location/Qualifiers
     source          1..6404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1778..1798
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1799..1828
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             1829..1852
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2010..2144
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2047..2066)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2101..2120
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2173..2338
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2522..2976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2609..2628)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2818..2839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3002..3106
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3107..3175,3176..3967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3325..3344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4138..4726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4627..4646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4880..4897
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5031..5255
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5322..5855)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6025..6030,6031..6047,6048..6053))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctacttaa gaaggagata tactatgccc cgagtggcct gggcggagag
      181 gctggttcgc gggctgcgag gtctctgggg aacaagactc atggaggaaa gcagcactaa
      241 ccgagagaaa taccttaaaa gtgttttacg ggaactggtc acatacctcc tttttctcat
      301 agtcttgtgc atcttgacct acggcatgat gagctccaat gtgtactact acacccggat
      361 gatgtcacag ctcttcctag acacccccgt gtccaaaacg gagaaaacta actttaaaac
      421 tctgtcttcc atggaagact tctggaagtt cacagaaggc tccttattgg atgggctgta
      481 ctggaagatg cagcccagca accagactga agctgacaac cgaagtttca tcttctatga
      541 gaacctgctg ttaggggttc cacgaatacg gcaactccga gtcagaaatg gatcctgctc
      601 tatcccccag gacttgagag atgaaattaa agagtgctat gatgtctact ctgtcagtag
      661 tgaagatagg gctccctttg ggccccgaaa tggaaccgct tggatctaca caagtgaaaa
      721 agacttgaat ggtagtagcc actggggaat cattgcaact tatagtggag ctggctatta
      781 tctggatttg tcaagaacaa gagaggaaac agctgcacaa gttgctagcc tcaagaaaaa
      841 tgtctggctg gaccgaggaa ccagggcaac ttttattgac ttctcagtgt acaacgccaa
      901 cattaacctg ttctgtgtgg tcaggttatt ggttgaattc ccagcaacag gtggtgtgat
      961 tccatcttgg caatttcagc ctttaaagct gatccgatat gtcacaactt ttgatttctt
     1021 cctggcagcc tgtgagatta tcttttgttt ctttatcttt tactatgtgg tggaagagat
     1081 attggaaatt cgcattcaca aactacacta tttcaggagt ttctggaatt gtctggatgt
     1141 tgtgatcgtt gtgctgtcag tggtagctat aggaattaac atatacagaa catcaaatgt
     1201 ggaggtgcta ctacagtttc tggaagatca aaatactttc cccaactttg agcatctggc
     1261 atattggcag atacagttca acaatatagc tgctgtcaca gtattttttg tctggattaa
     1321 gctcttcaaa ttcatcaatt ttaacaggac catgagccag ctctcgacaa ccatgtctcg
     1381 atgtgccaaa gacctgtttg gctttgctat tatgttcttc attattttcc tagcgtatgc
     1441 tcagttggca taccttgtct ttggcactca ggtcgatgac ttcagtactt tccaagagtg
     1501 tatcttcact caattccgta tcattttggg cgatatcaac tttgcagaga ttgaggaagc
     1561 taatcgagtt ttgggaccaa tttatttcac tacatttgtg ttctttatgt tcttcattct
     1621 tttgaatatg tttttggcta tcatcaatga tacttactct gaagtgaaat ctgacttggc
     1681 acagcagaaa gctgaaatgg aactctcaga tcttatcaga aagggctacc ataaagcttt
     1741 ggtcaaacta aaactgaaaa aaaataccgt ggatgcagag aacctctact tccaatcgca
     1801 ccatcatcac catcaccatc accaccatga ttacaaggat gacgacgata agtgaggatc
     1861 cgaattcgag ctccgtcgac aagcttgtcg agaagtacta gaggatcata atcagccata
     1921 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     1981 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     2041 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2101 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc
     2161 ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt
     2221 ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat
     2281 aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc
     2341 ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa
     2401 ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt catctcttcg
     2461 ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa tggcgaatgg
     2521 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2581 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2641 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     2701 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2761 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg
     2821 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     2881 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     2941 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     3001 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3061 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4261 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4741 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4801 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4861 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     4921 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct
     4981 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     5041 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     5101 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     5161 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     5221 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     5281 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     5341 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     5401 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     5461 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     5521 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     5581 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     5641 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     5701 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     5761 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     5821 ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
     5881 gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg
     5941 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     6001 gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt
     6061 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc
     6121 gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag
     6181 gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt
     6241 caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa
     6301 gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca ggactctagc
     6361 tatagttcta gtggttggct acgtatactc cggaatatta atag
//
