LOCUS       sequence-193306-        6206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6206)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AASS (AASSA-c013)
FEATURES             Location/Qualifiers
     source          1..6206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    1812..1946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1849..1868)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1903..1922
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1975..2140
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2324..2778
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2411..2430)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2620..2641
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2804..2908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2909..2977,2978..3769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3127..3146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3940..4528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4429..4448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4682..4699
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4833..5057
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5124..5657)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5827..5832,5833..5849,5850..5855))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg ggccaccatc atcatcatca ttcttctggt
      181 gtagatctgg gtaccgagaa cctgtacttc caatccatgg cattacctga taaatataaa
      241 tatatccaga cactccggga gagcagggaa cgtgctcagt cactttcaat gggcaccagg
      301 agaaaggttt tggttcttgg atctggctac atatctgagc ctgtattaga atatttatca
      361 agagatggca atatagaaat aacagtagga tctgacatga agaatcaaat tgaacagtta
      421 ggcaagaaat ataatattaa tcctgttagc atggacattt gtaaacaaga agagaagctg
      481 ggcttcttgg tggcaaaaca ggatcttgtc atcagcttgt tgccttatgt attgcaccct
      541 cttgtggcca aggcctgcat cacaaacaaa gttaacatgg tcactgcaag ctacatcaca
      601 ccagcactaa aagaattgga aaagagtgtg gaagatgctg gcatcacaat cattggtgaa
      661 ttgggattgg accctggtct ggatcacatg ttagcaatgg aatcaataga taaagccaag
      721 gaagtgggag ccacgattga atcatatatt tcctactgtg gtgggcttcc agcccctgaa
      781 cattcaaaca atccattgag atataaattt agctggagtc cagtgggagt tttgatgaat
      841 gtaatgcagt ctgccaccta tctgctcgat ggaaaggttg tgaatgttgc aggaggcatc
      901 tcctttcttg atgccgttac gtccatggat ttttttccag gattaaattt ggaaggctat
      961 cctaacagag acagtacgaa atatgctgag atttatggca tttcttctgc tcacactttg
     1021 ttgcggggga cactgagata taagggatat atgaaagctt tgaatggatt tgtaaaatta
     1081 ggtcttataa acagagaagc gcttcctgcc tttagacctg aggccaaccc tctcacctgg
     1141 aaacaactcc tctgtgacct agttgggatt tcaccctcct ctgagcatga tgtgttgaag
     1201 gaagctgttc ttaagaaact aggaggagac aatacccagt tggaggctgc tgaatggttg
     1261 ggcttacttg gggatgaaca agttcctcag gcagagtcca ttctggatgc cctctccaag
     1321 catttggtca tgaagctttc ctatggtcct gaagaaaaag atatgattgt gatgagagac
     1381 agctttggaa tcagacatcc ttctggacat ttagaacata aaacgattga tcttgtggct
     1441 tatggggaca tcaatggctt ttcagccatg gctaaaaccg tggggttacc caccgccatg
     1501 gcagccaaaa tgttgcttga tggtgaaatt ggagccaaag gcctaatggg gcccttttca
     1561 aaggagatct atggaccaat attggagcga attaaagcag aaggcattat atatactaca
     1621 cagagtacaa ttaaaccatg acagtaaagg tggatacgga tccgaattcg agctccgtcg
     1681 acaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt
     1741 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     1801 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1861 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1921 tcaatgtatc ttatcatgtc tggatctgat cactgatatc gcctaggaga tccgaaccag
     1981 ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac
     2041 gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat
     2101 ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg
     2161 ttatgttttt aatcaaacat cctgccaact ccatgtgaca aaccgtcatc ttcggctact
     2221 ttttctctgt cacagaatga aaatttttct gtcatctctt cgttattaat gtttgtaatt
     2281 gactgaatat caacgcttat ttgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     2341 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2401 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2461 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     2521 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2581 gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag tggactcttg ttccaaactg
     2641 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     2701 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     2761 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2821 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2881 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2941 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3001 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3061 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3121 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3181 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3241 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3301 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3361 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3421 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3481 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3541 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3601 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3661 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3721 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3781 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3841 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3901 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3961 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4021 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4081 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4141 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4201 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4261 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4321 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4381 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4441 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4501 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4561 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4621 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4681 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     4741 tctgtgcggt atttcacacc gcatagacca gccgcgtaac ctggcaaaat cggttacggt
     4801 tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg
     4861 aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact
     4921 gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa
     4981 ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gccaagggca
     5041 tggtaaagac tatattcgcg gcgttgtgac aatttaccga acaactccgc ggccgggaag
     5101 ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc
     5161 acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct
     5221 gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag cagattgatg
     5281 agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag
     5341 atctcactac gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc
     5401 gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg
     5461 taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa
     5521 agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg
     5581 atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     5641 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     5701 cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     5761 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
     5821 cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt catccgtttc
     5881 cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg
     5941 ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc ggccttgctg
     6001 ttcttctacg gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct
     6061 cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt
     6121 tttctggaag gcgagcatcg tttgttcgcc caggactcta gctatagttc tagtggttgg
     6181 ctacgtatac tccggaatat taatag
//
