LOCUS       sequence-193326-       11470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of human VE-cadherin, C-terminally tagged with
            mCitrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, 
            Betzig E, Meyer T
  TITLE     Engulfed cadherin fingers are polarized junctional structures 
            between collectively migrating endothelial cells.
  JOURNAL   Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub
            2016 Nov 14.
  PUBMED    27842057
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3438"; 
            journalName: "Nat Cell Biol"; date: "2016-12"; volume: "18"; issue: 
            "12"; pages: "1311-1323"
COMMENT     Sequence Label: pLV-CDH5-mCitrine-IRES-puro
FEATURES             Location/Qualifiers
     source          1..11470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     142..164
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     226..245
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             complement(2319..2330)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2644..2668)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2676..2685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2682..2684,2685..2687,2688..3401)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2727..2748)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2988..3007)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3335..3356
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3416..3440)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3558..4107
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3702..3719)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3929..3948
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4110..4709
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4110..4129)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4606..4626
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4733..5321
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4786..4806)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5204..5215)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5528..6161
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6289..6305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6289..6305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6302..6324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6313..6329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6337..6343,6344..6361,6362..6367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6382..6403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6520..6537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6691..7279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6771..6790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7450..8241,8242..8310))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8073..8092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8311..8415)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8463..8597
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8500..8519)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8554..8573
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8765..9398
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9445..9570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10067..10300
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10485..10529
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10792..10907
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        10970..11349
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
       61 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
      121 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
      181 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
      241 ttgtgctgtc tcatcatttt ggcaaagaat tcaccatgca gaggctcatg atgctcctcg
      301 ccacatcggg cgcctgcctg ggcctgctgg cagtggcagc agtggcagca gcaggtgcta
      361 accctgccca acgggacacc cacagcctgc tgcccaccca ccggcgccaa aagagagatt
      421 ggatttggaa ccagatgcac attgatgaag agaaaaacac ctcacttccc catcatgtag
      481 gcaagatcaa gtcaagcgtg agtcgcaaga atgccaagta cctgctcaaa ggagaatatg
      541 tgggcaaggt cttccgggtc gatgcagaga caggagacgt gttcgccatt gagaggctgg
      601 accgggagaa tatctcagag taccacctca ctgctgtcat tgtggacaag gacactggcg
      661 aaaacctgga gactccttcc agcttcacca tcaaagttca tgacgtgaac gacaactggc
      721 ctgtgttcac gcatcggttg ttcaatgcgt ccgtgcctga gtcgtcggct gtggggacct
      781 cagtcatctc tgtgacagca gtggatgcag acgaccccac tgtgggagac cacgcctctg
      841 tcatgtacca aatcctgaag gggaaagagt attttgccat cgataattct ggacgtatta
      901 tcacaataac gaaaagcttg gaccgagaga agcaggccag gtatgagatc gtggtggaag
      961 cgcgagatgc ccagggcctc cggggggact cgggcacggc caccgtgctg gtcactctgc
     1021 aagacatcaa tgacaacttc cccttcttca cccagaccaa gtacacattt gtcgtgcctg
     1081 aagacacccg tgtgggcacc tctgtgggct ctctgtttgt tgaggaccca gatgagcccc
     1141 agaaccggat gaccaagtac agcatcttgc ggggcgacta ccaggacgct ttcaccattg
     1201 agacaaaccc cgcccacaac gagggcatca tcaagcccat gaagcctctg gattatgaat
     1261 acatccagca atacagcttc atcgtcgagg ccacagaccc caccatcgac ctccgataca
     1321 tgagccctcc cgcgggaaac agagcccagg tcattatcaa catcacagat gtggacgagc
     1381 cccccatttt ccagcagcct ttctaccact tccagctgaa ggaaaaccag aagaagcctc
     1441 tgattggcac agtgctggcc atggaccctg atgcggctag gcatagcatt ggatactcca
     1501 tccgcaggac cagtgacaag ggccagttct tccgagtcac aaaaaagggg gacatttaca
     1561 atgagaaaga actggacaga gaagtctacc cctggtataa cctgactgtg gaggccaaag
     1621 aactggattc cactggaacc cccacaggaa aagaatccat tgtgcaagtc cacattgaag
     1681 ttttggatga gaatgacaat gccccggagt ttgccaagcc ctaccagccc aaagtgtgtg
     1741 agaacgctgt ccatggccag ctggtcctgc agatctccgc aatagacaag gacataacac
     1801 cacgaaacgt gaagttcaaa ttcaccttga atactgagaa caactttacc ctcacggata
     1861 atcacgataa cacggccaac atcacagtca agtatgggca gtttgaccgg gagcatacca
     1921 aggtccactt cctacccgtg gtcatctcag acaatgggat gccaagtcgc acgggcacca
     1981 gcacgctgac cgtggccgtg tgcaagtgca acgagcaggg cgagttcacc ttctgcgagg
     2041 atatggccgc ccaggtgggc gtgagcatcc aggcagtggt agccatctta ctctgcatcc
     2101 tcaccatcac agtgatcacc ctgctcatct tcctgcggcg gcggctccgg aagcaggccc
     2161 gcgcgcacgg caagagcgtg ccggagatcc acgagcagct ggtcacctac gacgaggagg
     2221 gcggcggcga gatggacacc accagctacg atgtgtcggt gctcaactcg gtgcgccgcg
     2281 gcggggccaa gcccccgcgg cccgcgctgg acgcccggcc ttccctctat gcgcaggtgc
     2341 agaagccacc gaggcacgcg cctggggcac acggagggcc cggggagatg gcagccatga
     2401 tcgaggtgaa gaaggacgag gcggaccacg acggcgacgg ccccccctac gacacgctgc
     2461 acatctacgg ctacgagggc tccgagtcca tagccgagtc cctcagctcc ctgggcaccg
     2521 actcatccga ctctgacgtg gattacgact tccttaacga ctggggaccc aggtttaaga
     2581 tgctggctga gctgtacggc tcggaccccc gggaggagct gctgtataag ggtgggcgcg
     2641 ccgacccagc tttcttgtac aaagtggtac cggtcgccac catggtgagc aagggcgagg
     2701 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2761 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     2821 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
     2881 acggcctgat gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2941 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3001 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3061 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3121 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     3181 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     3241 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca
     3301 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3361 ccgccgggat cactctcggc atggacgagc tgtacaagta actcgagata tctagaccca
     3421 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctctag agtcgacggg
     3481 ccgcggtaac aattgttaac taacttaagc tagcaacggt ttccctctag cgggatcaat
     3541 tccgcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     3601 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     3661 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3721 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     3781 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     3841 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     3901 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     3961 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     4021 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     4081 ggttttcctt tgaaaaacac gataatacca tgaccgagta caagcccacg gtgcgcctcg
     4141 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     4201 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     4261 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     4321 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     4381 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     4441 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     4501 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     4561 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     4621 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     4681 ggtgcatgac ccgcaagccc ggtgcctgac ccgggcgggg cgcgtctgga acaatcaacc
     4741 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4801 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4861 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4921 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4981 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5041 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5101 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     5161 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5221 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5281 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
     5341 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     5401 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     5461 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5521 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     5581 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     5641 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     5701 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     5761 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     5821 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     5881 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     5941 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     6001 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6061 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6121 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     6181 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca
     6241 ttgctagcgt ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     6301 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6361 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6421 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6481 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6541 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6601 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6661 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6721 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6781 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6841 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6901 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6961 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7021 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7081 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7141 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7201 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7261 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7321 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7381 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7441 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7501 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7561 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7621 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7681 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7741 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7801 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7861 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7921 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7981 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8041 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8101 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8161 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8221 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8281 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8341 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8401 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     8461 tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8521 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8581 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
     8641 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
     8701 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     8761 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     8821 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     8881 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     8941 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     9001 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     9061 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     9121 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     9181 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     9241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     9421 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     9481 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     9541 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     9601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9901 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     9961 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
    10021 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
    10081 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
    10141 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
    10201 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
    10261 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
    10321 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
    10381 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
    10441 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
    10501 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
    10561 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
    10621 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
    10681 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
    10741 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc ctttaaaaga
    10801 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
    10861 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
    10921 agggacagca gagatccagt ttatcgatga ggccctttcg tcttcactcg acattgatta
    10981 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
    11041 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
    11101 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
    11161 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
    11221 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
    11281 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    11341 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
    11401 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
    11461 gggggggggg
//
