LOCUS       sequence-193327-       11432 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of human VE-cadherin, C-terminally tagged with
            mRuby3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, 
            Betzig E, Meyer T
  TITLE     Engulfed cadherin fingers are polarized junctional structures 
            between collectively migrating endothelial cells.
  JOURNAL   Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub
            2016 Nov 14.
  PUBMED    27842057
REFERENCE   2  (bases 1 to 11432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3438"; 
            journalName: "Nat Cell Biol"; date: "2016-12"; volume: "18"; issue: 
            "12"; pages: "1311-1323"
COMMENT     Sequence Label: pLV-CDH5-mRuby3-IRES-puro
FEATURES             Location/Qualifiers
     source          1..11432
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     292..314
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     376..395
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             complement(2469..2480)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2794..2818)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2826..3536
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    3640..4189
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3784..3801)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4011..4030
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4192..4791
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4192..4211)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4688..4708
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4815..5403
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4868..4888)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5286..5297)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5610..6243
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6371..6387)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6371..6387)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6384..6406)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6395..6411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6419..6425,6426..6443,6444..6449))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6464..6485
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6602..6619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6773..7361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6853..6872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7532..8323,8324..8392))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8155..8174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8393..8497)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8545..8679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8582..8601)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8636..8655
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8847..9480
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9527..9652
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10149..10382
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10567..10611
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10874..10989
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        11052..11431
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
       61 gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
      121 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
      181 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
      241 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
      301 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
      361 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
      421 tcaccatgca gaggctcatg atgctcctcg ccacatcggg cgcctgcctg ggcctgctgg
      481 cagtggcagc agtggcagca gcaggtgcta accctgccca acgggacacc cacagcctgc
      541 tgcccaccca ccggcgccaa aagagagatt ggatttggaa ccagatgcac attgatgaag
      601 agaaaaacac ctcacttccc catcatgtag gcaagatcaa gtcaagcgtg agtcgcaaga
      661 atgccaagta cctgctcaaa ggagaatatg tgggcaaggt cttccgggtc gatgcagaga
      721 caggagacgt gttcgccatt gagaggctgg accgggagaa tatctcagag taccacctca
      781 ctgctgtcat tgtggacaag gacactggcg aaaacctgga gactccttcc agcttcacca
      841 tcaaagttca tgacgtgaac gacaactggc ctgtgttcac gcatcggttg ttcaatgcgt
      901 ccgtgcctga gtcgtcggct gtggggacct cagtcatctc tgtgacagca gtggatgcag
      961 acgaccccac tgtgggagac cacgcctctg tcatgtacca aatcctgaag gggaaagagt
     1021 attttgccat cgataattct ggacgtatta tcacaataac gaaaagcttg gaccgagaga
     1081 agcaggccag gtatgagatc gtggtggaag cgcgagatgc ccagggcctc cggggggact
     1141 cgggcacggc caccgtgctg gtcactctgc aagacatcaa tgacaacttc cccttcttca
     1201 cccagaccaa gtacacattt gtcgtgcctg aagacacccg tgtgggcacc tctgtgggct
     1261 ctctgtttgt tgaggaccca gatgagcccc agaaccggat gaccaagtac agcatcttgc
     1321 ggggcgacta ccaggacgct ttcaccattg agacaaaccc cgcccacaac gagggcatca
     1381 tcaagcccat gaagcctctg gattatgaat acatccagca atacagcttc atcgtcgagg
     1441 ccacagaccc caccatcgac ctccgataca tgagccctcc cgcgggaaac agagcccagg
     1501 tcattatcaa catcacagat gtggacgagc cccccatttt ccagcagcct ttctaccact
     1561 tccagctgaa ggaaaaccag aagaagcctc tgattggcac agtgctggcc atggaccctg
     1621 atgcggctag gcatagcatt ggatactcca tccgcaggac cagtgacaag ggccagttct
     1681 tccgagtcac aaaaaagggg gacatttaca atgagaaaga actggacaga gaagtctacc
     1741 cctggtataa cctgactgtg gaggccaaag aactggattc cactggaacc cccacaggaa
     1801 aagaatccat tgtgcaagtc cacattgaag ttttggatga gaatgacaat gccccggagt
     1861 ttgccaagcc ctaccagccc aaagtgtgtg agaacgctgt ccatggccag ctggtcctgc
     1921 agatctccgc aatagacaag gacataacac cacgaaacgt gaagttcaaa ttcaccttga
     1981 atactgagaa caactttacc ctcacggata atcacgataa cacggccaac atcacagtca
     2041 agtatgggca gtttgaccgg gagcatacca aggtccactt cctacccgtg gtcatctcag
     2101 acaatgggat gccaagtcgc acgggcacca gcacgctgac cgtggccgtg tgcaagtgca
     2161 acgagcaggg cgagttcacc ttctgcgagg atatggccgc ccaggtgggc gtgagcatcc
     2221 aggcagtggt agccatctta ctctgcatcc tcaccatcac agtgatcacc ctgctcatct
     2281 tcctgcggcg gcggctccgg aagcaggccc gcgcgcacgg caagagcgtg ccggagatcc
     2341 acgagcagct ggtcacctac gacgaggagg gcggcggcga gatggacacc accagctacg
     2401 atgtgtcggt gctcaactcg gtgcgccgcg gcggggccaa gcccccgcgg cccgcgctgg
     2461 acgcccggcc ttccctctat gcgcaggtgc agaagccacc gaggcacgcg cctggggcac
     2521 acggagggcc cggggagatg gcagccatga tcgaggtgaa gaaggacgag gcggaccacg
     2581 acggcgacgg ccccccctac gacacgctgc acatctacgg ctacgagggc tccgagtcca
     2641 tagccgagtc cctcagctcc ctgggcaccg actcatccga ctctgacgtg gattacgact
     2701 tccttaacga ctggggaccc aggtttaaga tgctggctga gctgtacggc tcggaccccc
     2761 gggaggagct gctgtataag ggtgggcgcg ccgacccagc tttcttgtac aaagtggtac
     2821 cggtcatggt gtctaagggc gaagagctga tcaaggaaaa tatgcgtatg aaggtggtca
     2881 tggaaggttc ggtcaacggc caccaattca aatgcacagg tgaaggagaa ggcagaccgt
     2941 acgagggagt gcaaaccatg aggatcaaag tcatcgaggg aggacccctg ccatttgcct
     3001 ttgacattct tgccacgtcg ttcatgtatg gcagccgtac ctttatcaag tacccggccg
     3061 acatccctga tttctttaaa cagtcctttc ctgagggttt tacttgggaa agagttacga
     3121 gatacgaaga tggtggagtc gtcaccgtca cgcaggacac cagccttgag gatggcgagc
     3181 tcgtctacaa cgtcaaggtc agaggggtaa actttccctc caatggtccc gtgatgcaga
     3241 agaagaccaa gggttgggag cctaatacag agatgatgta tccagcagat ggtggtctga
     3301 gaggatacac tgacatcgca ctgaaagttg atggtggtgg ccatctgcac tgcaacttcg
     3361 tgacaactta caggtcaaaa aagaccgtcg ggaacatcaa gatgcccggt gtccatgccg
     3421 ttgatcaccg cctggaaagg atcgaggaga gtgacaatga aacctacgta gtgcaaagag
     3481 aagtggcagt tgccaaatac agcaaccttg gtggtggcat ggacgagctg tacaagtaag
     3541 cggccgctct agagtcgacg ggccgcggta acaattgtta actaacttaa gctagcaacg
     3601 gtttccctct agcgggatca attccgcccc ccccccctaa cgttactggc cgaagccgct
     3661 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     3721 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     3781 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     3841 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     3901 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     3961 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     4021 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     4081 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     4141 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac catgaccgag
     4201 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     4261 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     4321 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     4381 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     4441 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     4501 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     4561 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     4621 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     4681 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     4741 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acccgggcgg
     4801 ggcgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4861 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4921 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4981 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5041 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5101 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5161 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     5221 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5281 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5341 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5401 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     5461 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     5521 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     5581 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
     5641 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     5701 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     5761 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     5821 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     5881 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     5941 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     6001 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     6061 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6121 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6181 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6241 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6301 cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg
     6361 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6421 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6481 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6541 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6601 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6661 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6721 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6781 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6841 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6901 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6961 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7021 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7081 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7141 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7201 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7261 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7321 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7381 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7441 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7501 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7561 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7621 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7681 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7741 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7801 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7861 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7921 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7981 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8041 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8101 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8161 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8221 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8281 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8341 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8401 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8461 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8521 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
     8581 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8641 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     8701 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     8761 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     8821 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     8881 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     8941 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     9001 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     9061 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     9121 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     9181 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     9241 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     9301 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9361 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9421 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9481 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     9541 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     9601 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     9661 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     9721 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     9781 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     9841 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     9901 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     9961 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
    10021 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    10081 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    10141 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    10201 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    10261 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    10321 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    10381 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    10441 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    10501 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    10561 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    10621 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    10681 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    10741 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    10801 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
    10861 cgacggtatc gcctttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
    10921 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
    10981 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttatcgat gaggcccttt
    11041 cgtcttcact cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    11101 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    11161 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    11221 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    11281 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    11341 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    11401 catctacgta ttagtcatcg ctattaccat gg
//
