LOCUS       sequence-19333-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf262
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             474..515
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             525..542
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(565..582)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    571..795
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cgagctcgga tccgtaccct tcaccgaatt ccagctgacc accatggcat ctttattata
       61 tcttatttta tttttgttat tcgtatgtat ttcttattat tttacatatt atccgaccaa
      121 taaacttcag gcagctgtaa tggaaacaga tagagaaaat gctattatta gacagcgaaa
      181 tgatgaaata ccgactagaa cattagatac agctatattt accgatgcat caaccgtcgc
      241 gagtgcgcaa atacacctat attataattc caatattggt aaaattataa tgtcacttaa
      301 tggtaaaaaa cacaccttta atttatacga tgataacgac atacgaacat tacttcctat
      361 tttactcctt agtaaatgag caattcccgg ggatccgaat aaagggtcaa gacaattctg
      421 cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc
      481 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc
      541 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      601 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      661 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
      721 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
      781 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
      841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      901 ctacacttgc cagcgccct
//
