LOCUS       sequence-193338-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the human La antigen for use in ALARM NMR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Dahlin JL, Nelson KM, Strasser JM, Barsyte-Lovejoy D, Szewczyk MM, 
            Organ S, Cuellar M, Singh G, Shrimp JH, Nguyen N, Meier JL, 
            Arrowsmith CH, Brown PJ, Baell JB, Walters MA
  TITLE     Assay interference and off-target liabilities of reported histone 
            acetyltransferase inhibitors.
  JOURNAL   Nat Commun. 2017 Nov 15;8(1):1527. doi: 10.1038/s41467-017-01657-3.
  PUBMED    29142305
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-15"; volume: "8(1):1527. doi"; pages: " 
            10.1038/s41467-017-01657-3"
COMMENT     Sequence Label: ALARM NMR
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             61..93
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             103..123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             823..840
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggctagca tgactggtgg acagcaaatg ggtcgggatc tggaaaacct gtattttcag
      121 ggcatggatc cggtgactga tgagtataaa aatgatgtaa aaaacagatc tgtttatatt
      181 aaaggcttcc caactgatgc aactcttgat gacataaaag aatggttaga agataaaggt
      241 caagtactaa atattcagat gagaagaaca ttgcataaag catttaaggg atcaattttt
      301 gttgtgtttg atagcattga atctgctaag aaatttgtag agacccctgg ccagaagtac
      361 aaagaaacag acctgctaat acttttcaag gacgattact ttgccaaaaa aaatgaagaa
      421 agaaaacaaa ataaagtgga agctaaatta agagctaaac aggagcaaga agcaaaacaa
      481 aagttagaag aagatgctga aatgaaatct ctagaagaaa agattggatg cttgctgaaa
      541 ttttcgggtg atttagatga tcagacctgt agagaagatt tacacatact tttttcaaat
      601 catggtgaaa taaaatggat agacttcgtc agaggagcaa aagaggggat aattctattt
      661 aaagaaaaag ccaaggaagc attgggtaaa gccaaagatg caaataatgg taacctacaa
      721 ttaaggaaca aagaagtgaa ttgggaagta ctagaaggag aggtggaaaa agaagcactg
      781 aagaaaataa tagaagacca acaagaagcg gccgcactcg agcaccacca ccaccaccac
//
