LOCUS       sequence-193339-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the human La antigen for use in ALARM NMR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Dahlin JL, Nelson KM, Strasser JM, Barsyte-Lovejoy D, Szewczyk MM, 
            Organ S, Cuellar M, Singh G, Shrimp JH, Nguyen N, Meier JL, 
            Arrowsmith CH, Brown PJ, Baell JB, Walters MA
  TITLE     Assay interference and off-target liabilities of reported histone 
            acetyltransferase inhibitors.
  JOURNAL   Nat Commun. 2017 Nov 15;8(1):1527. doi: 10.1038/s41467-017-01657-3.
  PUBMED    29142305
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-11-15"; volume: "8(1):1527. doi"; pages: " 
            10.1038/s41467-017-01657-3"
COMMENT     Sequence Label: ALARM NMR
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(718..738)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(748..780)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(784..801)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(811..828)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtggtggtgg tggtggtgct cgagtgcggc cgcttcttgt tggtcttcta ttattttctt
       61 cagtgcttct ttttccacct ctccttctag tacttcccaa ttcacttctt tgttccttaa
      121 ttgtaggtta ccattatttg catctttggc tttacccaat gcttccttgg ctttttcttt
      181 aaatagaatt atcccctctt ttgctcctct gacgaagtct atccatttta tttcaccatg
      241 atttgaaaaa agtatgtgta aatcttctct acaggtctga tcatctaaat cacccgaaaa
      301 tttcagcaag catccaatct tttcttctag agatttcatt tcagcatctt cttctaactt
      361 ttgttttgct tcttgctcct gtttagctct taatttagct tccactttat tttgttttct
      421 ttcttcattt tttttggcaa agtaatcgtc cttgaaaagt attagcaggt ctgtttcttt
      481 gtacttctgg ccaggggtct ctacaaattt cttagcagat tcaatgctat caaacacaac
      541 aaaaattgat cccttaaatg ctttatgcaa tgttcttctc atctgaatat ttagtacttg
      601 acctttatct tctaaccatt cttttatgtc atcaagagtt gcatcagttg ggaagccttt
      661 aatataaaca gatctgtttt ttacatcatt tttatactca tcagtcaccg gatccatgcc
      721 ctgaaaatac aggttttcca gatcccgacc catttgctgt ccaccagtca tgctagccat
      781 atggctgccg cgcggcacca ggccgctgct gtgatgatga tgatgatggc tgctgcccat
//
