LOCUS       sequence-193347-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mouse CBP ZZ domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Legge GB, Martinez-Yamout MA, Hambly DM, Trinh T, Lee BM, Dyson HJ, 
            Wright PE
  TITLE     ZZ domain of CBP: an unusual zinc finger fold in a protein 
            interaction module.
  JOURNAL   J Mol Biol. 2004 Oct 29;343(4):1081-93.
  PUBMED    15476823
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2004-10-29"; volume: "343(4)"; pages: "1081-93"
COMMENT     Sequence Label: pET-ZZ(1700-1751)
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             208..225
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(278..296)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      292..339
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      376..831
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(463..482)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     673..694
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        857..961
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctttaagaag gagatataca tatgggccag gaccgctttg tttatacctg caatgagtgc
       61 aaacaccatg tggaaacacg ctggcactgc actgtgtgtg aggactatga cctttgtatc
      121 aattgctaca acacaaagag ccacacccat aagatggtga agtgggggct aggcctagat
      181 taatagaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct
      241 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      301 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      361 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      421 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      481 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      541 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      601 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      661 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      721 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      781 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
      841 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      901 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      961 tatgagtatt caacatttcc gtgtcgccct tattcc
//
