LOCUS       sequence-193350-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial coexpression of CBP NCBD and NCoA3 binding domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Demarest SJ, Martinez-Yamout M, Chung J, Chen H, Xu W, Dyson HJ, 
            Evans RM, Wright PE
  TITLE     Mutual synergistic folding in recruitment of CBP/p300 by p160 
            nuclear receptor coactivators.
  JOURNAL   Nature. 2002 Jan 31;415(6871):549-53.
  PUBMED    11823864
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2002-01-31"; volume: "415(6871)"; pages: "549-53"
COMMENT     Sequence Label: pET-NCBD + ACTR
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             241..263
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             511..528
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(581..599)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      595..642
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(766..785)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgcca aacaggagca tctcgccaag
       61 tgccctgcaa gacctgctac ggaccctaaa gtcacccagc tctcctcagc agcagcagca
      121 ggtgctgaac atccttaaat caaacccaca gctaatggca gctttcatca aacagcgcac
      181 agccaagtat gtggccaatc agcctggcat gcagtgataa ggatcccctc tagaaataat
      241 tttgtttaac tttaagaagg agatatacca tgggcactca aaataggcct cttcttagga
      301 attccctgga tgatcttgtt gggccacctt ccaacctgga aggccagagt gacgaaagag
      361 cattattgga ccagctgcac actcttctca gcaacacaga tgccacaggc ctggaagaaa
      421 ttgacagagc tttgggcatt cctgaacttg tcaatcaggg acaggcatta gagcccaaac
      481 aggattgata agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      541 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      601 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      661 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      721 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      781 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      841 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
      901 atggttcacg tagtgggcca tcgccctgat agacggtttt
//
