LOCUS       sequence-193355-        5139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  It contains ~0.8 Kb of the miR-183-96-182 cluster promoter. Is the 
            minimal region that contains the maximal promoter activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Segura MF, Jubierre L, Li S, Soriano A, Koetz L, Gaziel-Sovran A, 
            Masanas M, Kleffman K, Dankert JF, Walsh MJ, Hernando E
  TITLE     Kruppel-like factor 4 (KLF4) regulates the miR-183~96~182 cluster 
            under physiologic and pathologic conditions.
  JOURNAL   Oncotarget. 2017 Apr 18;8(16):26298-26311. doi: 
            10.18632/oncotarget.15459.
  PUBMED    28412746
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget"; journalName: "Oncotarget"; date: "2017-04-18-
            18"; volume: "8"; issue: "16"; pages: "26298-26311"
COMMENT     Sequence Label: pGL4-Cluster Promoter Delta 1.1
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..34
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        999..1030
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1057..1066
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1063..2715
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2759..2880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2770..2789)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2824..2843
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3128..3145)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3299..3887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3379..3398)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4087..4878,4879..4947))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5052..5100
                     /note="synthetic polyadenylation signal"
     misc_feature    join(5114..5139,1..66)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
       61 ttctctggcc taactggccg gtaccgagct cggatccact agtaacggcc gccagtgtgc
      121 tggaattgca gtggggggag ggggagatgg gaacgggtct gggggcggct cccctccccc
      181 tccctctctt gggacagaca gcctttgttc ccggcggcgg cggcagcggt ggccagacac
      241 ctgtgaggcc gcagaactgg ccgagggtgg gcgtgtgagg acatggaggc cccttgtccc
      301 cattccagcc ctgcgcccct gggtccgggt gcgccccctg agacaggtca gccttgagca
      361 cctttctgcg gggagactgc agagatcgtc cagtggccct acggtgcgcc ccagctccca
      421 acgcacttcc cctcccccct cccagggtcg gatttccaag cacgccacgc cacacacacc
      481 tcggctagct ccgggctcag agggcgctgg ggtggcaggg cgtccgggcg cacctcggct
      541 agcggcaccc tagcccgagg gcaggaggcg gggatagaag gcgggcgacg ggaccccttg
      601 gggccagacc cggccgggtt gggcaagcag gcggggctcc cggcgtctcc gcagtcccgg
      661 acgggcgtgc cctgcgccgc gcaccaaccg gagccacatt caaggtgaat catccccggc
      721 ccgccccctg cccctccccg cctcgcggca cctgagccaa tggcgctgcg cgaggggcgg
      781 gcggggcccg gggcgggggg cggtaaaacc ctcctggagt cattctctgt gcactccgtc
      841 gagcccccgc gcagccttgc cggagccgca gggcctccgt ccagccgcgc ggtccgggca
      901 gcccgcgccc cctgttcttc cggtcctcgg gcccaggtac ctgagctcgc tagcctcgag
      961 gatatcaaga tctggcctcg gcggccaagc ttagacacta gagggtatat aatggaagct
     1021 cgacttccag cttggcaatc cggtactgtt ggtaaagcca ccatggaaga tgccaaaaac
     1081 attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg
     1141 cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat
     1201 atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct
     1261 atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg
     1321 cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac
     1381 gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta
     1441 ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata
     1501 caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc
     1561 ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc
     1621 gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag
     1681 ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc
     1741 ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt tcaccacggc
     1801 ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt gctcatgtac
     1861 cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca atctgccctg
     1921 ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa gtacgaccta
     1981 agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc
     2041 gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac agaaacaacc
     2101 agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg caaggtggtg
     2161 cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg tgtgaaccag
     2221 cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa caaccccgag
     2281 gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg
     2341 gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc
     2401 taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac
     2461 gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg
     2521 ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt
     2581 acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg
     2641 accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc
     2701 aagatcgccg tgtaataatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     2761 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2821 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2881 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2941 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc
     3001 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     3061 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     3121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3721 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3961 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4021 caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg
     4081 cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc
     4141 gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc
     4201 aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc
     4261 aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc
     4321 catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt
     4381 ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc
     4441 ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag
     4501 aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt
     4561 gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg
     4621 cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc
     4681 aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa
     4741 agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt
     4801 gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt
     4861 caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag
     4921 tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta
     4981 tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt
     5041 ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     5101 aatcgatagt actaacatac gctctccatc aaaacaaaa
//
