LOCUS       sequence-193358-       11940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human KLF4 lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11940)
  AUTHORS   Segura MF, Jubierre L, Li S, Soriano A, Koetz L, Gaziel-Sovran A, 
            Masanas M, Kleffman K, Dankert JF, Walsh MJ, Hernando E
  TITLE     Kruppel-like factor 4 (KLF4) regulates the miR-183~96~182 cluster 
            under physiologic and pathologic conditions.
  JOURNAL   Oncotarget. 2017 Apr 18;8(16):26298-26311. doi: 
            10.18632/oncotarget.15459.
  PUBMED    28412746
REFERENCE   2  (bases 1 to 11940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget"; journalName: "Oncotarget"; date: "2017-04-18-
            18"; volume: "8"; issue: "16"; pages: "26298-26311"
COMMENT     Sequence Label: pEIGW-human KLF4
FEATURES             Location/Qualifiers
     source          1..11940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2032..2149
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2206..2417
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    join(4375..4949,4950..4952,4953..4961)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4542..4559)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4769..4788
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4962..4964,4965..4967,4968..5681)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4962..4964,4965..4967,4968..5678)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5007..5028)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5268..5287)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5615..5636
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5740..5756)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5741..5757)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5770..6358
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5823..5843)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6241..6252)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6502..6535)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6555..6735
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6760..6778)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6761..6778)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7068..7087)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7301..7320)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7420..7442
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7480..7498)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7566..7670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7671..7739,7740..8531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7889..7908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8702..9290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9191..9210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9444..9461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9531..9551)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9536..9865
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9716..9851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10999..11064
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11194..11214
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11639..11773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11676..11695)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11730..11749
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2041 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2101 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcacga
     2161 gactagcctc gagaagcttg atcgatggct ccggtgcccg tcagtgggca gagcgcacat
     2221 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     2281 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2341 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2401 ttgccgccag aacacaggtg tcgtgacgcg gatcgggatc ggcctttacg gccggttacc
     2461 catcgtcgac ccacgcgtcc gctcggccaa tttggggttt tgggttttgg cttcgtttct
     2521 tctcttcgtt gactttgggg ttcaggtgcc ccagctgctt cgggctgccg aggaccttct
     2581 gggcccccac attaatgagg cagccacctg gcgagtctga catggctgtc agcgacgcgc
     2641 tgctcccatc tttctccacg ttcgcgtctg gcccggcggg aagggagaag acactgcgtc
     2701 aagcaggtgc cccgaataac cgctggcggg aggagctctc ccacatgaag cgacttcccc
     2761 cagtgcttcc cggccgcccc tatgacctgg cggcggcgac cgtggccaca gacctggaga
     2821 gcggcggagc cggtgcggct tgcggcggta gcaacctggc gcccctacct cggagagaga
     2881 ccgaggagtt caacgatctc ctggacctgg actttattct ctccaattcg ctgacccatc
     2941 ctccggagtc agtggccgcc accgtgtcct cgtcagcgtc agcctcctct tcgtcgtcgc
     3001 cgtcgagcag cggccctgcc agcgcgccct ccacctgcag cttcacctat ccgatccggg
     3061 ccgggaacga cccgggcgtg gcgccgggcg gcacgggcgg aggcctcctc tatggcaggg
     3121 agtccgctcc ccctccgacg gctcccttca acctggcgga catcaacgac gtgagcccct
     3181 cgggcggctt cgtggccgag ctcctgcggc cagaattgga cccggtgtac attccgccgc
     3241 agcagccgca gccgccaggt ggcgggctga tgggcaagtt cgtgctgaag gcgtcgctga
     3301 gcgcccctgg cagcgagtac ggcagcccgt cggtcatcag cgtcagcaaa ggcagccctg
     3361 acggcagcca cccggtggtg gtggcgccct acaacggcgg gccgccgcgc acgtgcccca
     3421 agatcaagca ggaggcggtc tcttcgtgca cccacttggg cgctggaccc cctctcagca
     3481 atggccaccg gccggctgca cacgacttcc ccctggggcg gcagctcccc agcaggacta
     3541 ccccgaccct gggtcttgag gaagtgctga gcagcaggga ctgtcaccct gccctgccgc
     3601 ttcctcccgg cttccatccc cacccggggc ccaattaccc atccttcctg cccgatcaga
     3661 tgcagccgca agtcccgccg ctccattacc aagagctcat gccacccggt tcctgcatgc
     3721 cagaggagcc caagccaaag aggggaagac gatcgtggcc ccggaaaagg accgccaccc
     3781 acacttgtga ttacgcgggc tgcggcaaaa cctacacaaa gagttcccat ctcaaggcac
     3841 acctgcgaac ccacacaggt gagaaacctt accactgtga ctgggacggc tgtggatgga
     3901 aattcgcccg ctcagatgaa ctgaccaggc actaccgtaa acacacgggg caccgcccgt
     3961 tccagtgcca aaaatgcgac cgagcatttt ccaggtcgga ccacctcgcc ttacacatga
     4021 agaggcattt ttaaatccca gacagtggat atgacccaca ctgccagaag agaattcagt
     4081 attttttact tttcacactg tcttcccgat gagggaagga gcccagccag aaagcactac
     4141 aatcatggtc aagttcccaa ctgagtcatc ttgtgagtgg ataatcagga aaaatgagga
     4201 atccaaaaga caaaaatcaa agaacagatg ggatctgtga ctggatcttc tatcattcca
     4261 attctaaatc cgacttgaat attcctggac ttacaaaatg ccaagggggt gactggaagt
     4321 tgtggatggg ctgcagtgat cacatatggt gacccggccc agttggccga tccgcccctc
     4381 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     4441 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     4501 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     4561 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     4621 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     4681 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     4741 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     4801 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg
     4861 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     4921 ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg
     4981 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     5041 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     5101 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     5161 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     5221 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     5281 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     5341 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     5401 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     5461 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     5521 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     5581 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     5641 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagct
     5701 agtcgagctc aagcttcgaa ttcgatatca agcttatcgc gataccgtcg acctcgaggg
     5761 aattccgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5821 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5881 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5941 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6001 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6061 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6121 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     6181 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6241 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6301 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     6361 cgggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6421 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6481 tgggatcaat tcaccatggg aataacttcg tatagcatac attatacgaa gttatgctgc
     6541 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6601 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6661 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6721 ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg
     6781 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta
     6841 caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac
     6901 tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca
     6961 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
     7021 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     7081 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     7141 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     7201 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     7261 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
     7321 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7381 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     7441 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     7501 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     7561 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7621 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7681 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7741 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7801 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7861 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7921 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7981 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8041 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8101 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8161 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8221 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8281 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8341 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8401 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8461 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8521 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8581 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8641 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8701 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8761 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8821 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8881 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8941 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9001 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9061 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9121 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9301 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9361 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9421 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9481 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
     9541 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9601 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9661 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     9721 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9781 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     9841 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
     9901 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
     9961 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
    10021 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
    10081 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
    10141 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
    10201 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
    10261 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
    10321 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
    10381 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
    10441 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
    10501 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
    10561 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
    10621 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
    10681 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
    10741 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
    10801 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
    10861 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
    10921 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
    10981 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
    11041 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
    11101 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
    11161 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
    11221 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
    11281 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
    11341 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
    11401 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
    11461 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
    11521 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
    11581 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    11641 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    11701 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    11761 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    11821 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
    11881 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
