LOCUS       sequence-19336-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf265
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             566..607
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             617..634
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(657..674)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    663..774
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctcggat ccgtaccctt caccgaattc cagctgacca cctcgtataa aatggaaaat
       61 gtgtttttct tttttacttt tacaaatgta tgttctctaa ccttagatcg tattaaatgg
      121 tctacatctt ttactaatat cttttttcct gattttgtta taacgctagg aataaaaata
      181 ttatctccta tacgatctac cttgatggac tcaatataca tgaacgagaa tttgaaaaag
      241 tctacaactt gccgcctaag ttctcccaac ttaatatacg atatggatat ttttggaaat
      301 ttatcatcaa aagagcgttc tataatagag tataactcgt cgtcaaataa tgtgtcttcc
      361 tctatttcaa tttctaaata tttgggtatg tttaatccgt actcactagc tagtttaaat
      421 atattaactg gagactgata ttttgatata gcatcttcca ttatccatct agcaattccc
      481 ggggatccga ataaagggtc aagacaattc tgcagatatc cagcacagtg gcggccgctc
      541 gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
      601 ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
      661 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
      721 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
      781 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca aggggggagg
      841 attgggaaga caatagcagg catg
//
