LOCUS       sequence-193360-       10776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expressing translational fusion between blue fluorescent 
            protein and Erk2 bearing the D319N mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10776)
  AUTHORS   Wilson MZ, Ravindran PT, Lim WA, Toettcher JE
  TITLE     Tracing Information Flow from Erk to Target Gene Induction Reveals 
            Mechanisms of Dynamic and Combinatorial Control.
  JOURNAL   Mol Cell. 2017 Sep 7;67(5):757-769.e5. doi: 
            10.1016/j.molcel.2017.07.016. Epub 2017 Aug 17.
  PUBMED    28826673
REFERENCE   2  (bases 1 to 10776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.07.016"; journalName: "Mol Cell"; date: 
            "2017-09-7- 7"; volume: "67"; issue: "5"; pages: "757-769.e5"
COMMENT     Sequence Label: pHR SFFVp BFP-Erk D319N
FEATURES             Location/Qualifiers
     source          1..10776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    243..360
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        514..922
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             997..1695
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             2749..2784
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
     misc_feature    2885..3473
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2938..2958)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3356..3367)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3536..3769
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3794..3812)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3795..3812)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4102..4121)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4455..4477
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4515..4533)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4601..4705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4706..4774,4775..5566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4924..4943)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5737..6325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6226..6245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6479..6496
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6566..6586)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6571..6900
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6751..6886
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8034..8099
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8229..8249
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8674..8808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8711..8730)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8765..8784
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8977..9610
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9657..9782
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10274..10507
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10692..10736
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
       61 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
      121 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
      181 acagatccat tcgattagtg aacggatctc gacggtcgcc aaatggcagt attcatccac
      241 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
      301 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
      361 cgggtttatt acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc
      421 ctgcagcccc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
      481 accccacctg taggttatgg caagctagct gcagtaacgc cattattgca aggcatggaa
      541 aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa aatagctaac
      601 gtagggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg gccaagaaca
      661 gatggtcacc gcagtttcgg ccccggcccg aggccaagag cagatggtcc ccagatatgg
      721 cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc caaggacctg
      781 aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc
      841 gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg cgccagtcct
      901 ccgacagact gagtcgcccg ggggggatct ggagctctcg agaattctca cgcgtcaagt
      961 ggagcaaggc aggtggacag tggatccgga gctaccatga gcgagctgat taaggagaac
     1021 atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc
     1081 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     1141 ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc
     1201 ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc
     1261 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     1321 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc
     1381 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac
     1441 cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc
     1501 catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa gaacctcaag
     1561 atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag
     1621 acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg
     1681 gggcacaagc ttaatgctca agcttcgaat tctgcagtcg acggtaccgc gggcccggag
     1741 atggtccgcg ggcaggtgtt cgacgtaggg ccgcgctaca ccaacctctc gtacatcgga
     1801 gaaggcgcct acggcatggt ttgctctgct tatgataatc tcaacaaagt tcgagttgct
     1861 atcaagaaaa tcagtccttt tgagcaccag acctactgtc aaagaaccct aagagagata
     1921 aaaatcttac tgcgcttcag acatgagaac atcattggca tcaatgacat catccgggca
     1981 ccaaccattg agcaaatgaa agatgtatat atagtacagg acctcatgga gacggacctt
     2041 tacaagctct tgaagacaca gcacctcagc aatgaccaca tctgctattt tctttatcag
     2101 atcctgagag ggctaaagta tatccattca gctaacgttc tgcaccgtga cctcaagcct
     2161 tccaacctcc tgctgaacac cacttgtgat ctcaagatct gtgactttgg ccttgcccgt
     2221 gttgcagatc cagatcatga tcacacaggg ttcttgacag agtacgtagc cacacgttgg
     2281 tacagagctc cagaaattat gttgaattcc aagggttata ccaagtccat tgatatttgg
     2341 tctgtgggct gcatcctggc agagatgcta tccaacaggc ctatcttccc aggaaagcat
     2401 taccttgacc agctgaatca catcctgggt attcttggat ctccatcaca ggaagatctg
     2461 aattgtataa taaatttaaa agctagaaac tatttgcttt ctctcccgca caaaaataag
     2521 gtgccatgga acaggttgtt cccaaatgct gactccaaag ctctggattt actggataaa
     2581 atgttgacat ttaaccctca caagaggatt gaagttgaac aggctctggc ccacccatac
     2641 ctggagcagt attatgaccc aagtaatgag cccattgctg aagcgccatt caagtttgac
     2701 atggagttgg acgacttacc taaggagaag ctcaaagaac tcatttttga agagactgct
     2761 agattccagc caggatacag atcttaagcg gccgcaactc ccacctgcaa catgcgtgac
     2821 tgactgaggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat
     2881 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2941 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3001 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3061 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3121 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3181 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3241 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3301 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3361 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3421 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     3481 ccgtcgacca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     3541 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     3601 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     3661 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     3721 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat
     3781 taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt
     3841 attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct
     3901 tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac
     3961 gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca
     4021 gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg
     4081 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     4141 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     4201 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
     4261 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa
     4321 tggtgcactc tcagtacaat ctagctctga tgccgcatag ttaagccagc cccgacaccc
     4381 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4441 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4501 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4561 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4621 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4681 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4741 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4801 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4861 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4921 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4981 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5041 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5101 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5161 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5221 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5281 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5341 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5401 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5461 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5521 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5581 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5641 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5701 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5761 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5821 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5881 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5941 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6001 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6061 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6121 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6181 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6241 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6301 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6361 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6421 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6481 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6541 ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6601 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6661 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6721 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6781 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6841 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6901 aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc
     6961 agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc
     7021 agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa
     7081 acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat
     7141 ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc
     7201 cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat
     7261 gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg
     7321 aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt
     7381 ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca
     7441 aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg
     7501 attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct
     7561 tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt
     7621 tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt
     7681 caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc
     7741 acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat
     7801 taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc
     7861 acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg
     7921 ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact
     7981 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
     8041 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
     8101 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
     8161 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
     8221 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
     8281 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
     8341 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct
     8401 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc
     8461 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt
     8521 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc
     8581 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     8641 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     8701 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8761 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     8821 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     8881 ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     8941 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     9001 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     9061 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     9121 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     9181 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     9241 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     9301 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     9361 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     9421 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9481 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9541 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9601 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     9661 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     9721 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     9781 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     9841 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     9901 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     9961 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
    10021 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
    10081 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggtgat cttcagacct
    10141 ggacgatata tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    10201 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    10261 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    10321 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    10381 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    10441 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    10501 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    10561 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    10621 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    10681 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    10741 attggtttaa cataacaaat tggctgtggt atataa
//
