LOCUS       sequence-193362-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mouse CBP TAZ1 domain as coexpression with 
            Hif1a TAD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Sugase K, Landes MA, Wright PE, Martinez-Yamout M
  TITLE     Overexpression of post-translationally modified peptides in 
            Escherichia coli by co-expression with modifying enzymes.
  JOURNAL   Protein Expr Purif. 2008 Feb;57(2):108-15. Epub 2007 Nov 1.
  PUBMED    18054500
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-02"; volume: "57(2)"; pages: "108-15. Epub
            2007 Nov 1"
COMMENT     Sequence Label: HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..234
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             259..276
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     RBS             462..484
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             819..836
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(889..907)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acgatgggcc atcatcatca tcatcacagc
       61 agcggcatga cttacaaact gatccttaac ggtaaaactt tgaaaggcga aaccactacc
      121 gaagctgttg atgctgcgac tgcagaaaaa gttttcaaac agtacgctaa cgacaacggt
      181 gttgacggtg aatggactta cgacgatgcg actaagacct tcaccgttac tgaagaattc
      241 agcagcggca gcagcggcct ggttccgcgt ggttcccata tgtctgattt agcatgtaga
      301 ctgctggggc aatcaatgga tgaaagtgga ttaccacagc tgaccagtta tgattgtgaa
      361 gttaatgctc ctatacaagg cagcagaaac ctactgcagg gtgaagaatt actcagagct
      421 ttggatcaag ttaactgata gggatcccct ctagaaataa ttttgtttaa ctttaagaag
      481 gagatatacc atggcaacag gccccacagc agaccctgaa aaacgcaaac tgatacagca
      541 gcagctggtt ctactgcttc atgcccacaa atgtcagaga cgagagcaag caaatggaga
      601 ggttcgagcc tgttctctcc cacactgtcg aaccatgaaa aacgttttga atcacatgac
      661 acattgtcag gctgggaaag cctgccaagt tgcccattgt gcatcttcac gacaaatcat
      721 ctctcattgg aagaactgca cacgacatga ctgtcctgtt tgcctccctt tgaaaaatgc
      781 cagtgacaag cgatagtaag cttgcggccg cactcgagca ccaccaccac caccactgag
      841 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      901 aactagcata accccttggg g
//
