LOCUS       sequence-193376-        9520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues 
            mutated to Arginine).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 9520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-FRT-TO-GFP-PALB2 KR
FEATURES             Location/Qualifiers
     source          1..9520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(990..992,993..995,996..1706)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(990..992,993..995,996..1706)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1035..1056)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1296..1315)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1643..1664
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1716..1740
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5306..5430)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5472..5489)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5478..5702
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5835..5854)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5986..6033
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6041..7063
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7193..7314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7230..7249)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7284..7303
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7363..7379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7363..7379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7376..7398)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7387..7403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7411..7417,7418..7435,7436..7441))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7456..7477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7594..7611)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7765..8353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7845..7864)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8524..9315,9316..9384))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9147..9166
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9385..9489)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggtgagcaa gggcgaggag ctgttcaccg
     1021 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1081 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1141 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1201 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1261 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1321 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1381 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1441 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1501 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1561 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1621 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1681 ctctcggcat ggacgagctg tacaagggcg cgcccacaag tttgtacaaa aaagcaggct
     1741 ccaccatgga cgagcctccc gggaagcccc tcagctgtga ggagagggaa aggttaaggg
     1801 agagattagc attcttgaga agggaataca gcaggacact agcccgcctt cagcgtgccc
     1861 aaagagctga aaggattagg cattctatta agaaaacagt agaagaacaa gattgtttgt
     1921 ctcagcagga tctctcaccg cagctaaaac actcagaacc taaaaataaa atatgtgttt
     1981 atgacaagtt acacatcaaa acccatcttg atgaagaaac tggagaaaag acatctatca
     2041 cacttgatgt tgggcctgag tcctttaacc ctggagatgg cccaggagga ttacctatac
     2101 aaagaacaga tgacacccaa gaacattttc cccacagggt cagtgaccct agtggtgagc
     2161 aaaagcagaa gctgccaagc agaagaaaga agcagcagaa gaggacattt atttcacagg
     2221 agagagactg tgtctttggc actgattcac tcagattgtc tgggaaaaga ctaaaggaac
     2281 aggaagaaat cagtagcaaa aatcctgcta gatcaccagt aactgaaata agaactcacc
     2341 ttttaagtct taaatctgaa cttccagatt ctccagaacc agttacagaa attaatgaag
     2401 acagtgtatt aattccacca actgcccaac cagaaaaagg tgttgataca ttcctaagaa
     2461 gacctaattt caccagggcg actacagttc ctttacagac tctatcagat agcggtagta
     2521 gtcagcacct tgaacacatt cctcctaaag gtagcagtga acttactact cacgacctaa
     2581 aaaacattag atttacttca cctgtaagtt tggaggcaca aggcaaaaaa atgactgtct
     2641 ctacagataa cctccttgta aataaagcta taagtaaaag tggccaactg cccacaagtt
     2701 ctaatttaga ggcaaatatt tcatgttctc taaatgaact cacctacaat aacttaccag
     2761 caaatgaaaa ccaaaactta aaagaacaaa atcaaacaga gaaatcttta aaatctccca
     2821 gtgacactct tgatggcagg aatgaaaatc ttcaggaaag tgagattcta agtcaaccta
     2881 agagtcttag cctggaagca acctctcctc tttctgcaga aaaacattct tgcacagtgc
     2941 ctgaaggcct tctgtttcct gcagaatatt atgttagaac aacacgaagc atgtccaatt
     3001 gccagaggaa agtagccgtg gaggctgtca ttcagagtca tttggatgtc aagaaaaaag
     3061 ggtttaaaaa taaaaataag gatgcaagta aaaatttaaa cctttccaat gaggaaactg
     3121 accaaagtga aattaggatg tctggcacat gcacaggaca accaagttca agaacctctc
     3181 agaaacttct ctcattaact aaagtcagct ctcccgctgg gcccactgaa gataatgact
     3241 tgtctaggaa ggcagttgcc caagcacctg gtagaagata cacaggaaaa agaaaatcag
     3301 cctgcacccc agcatcagat cattgtgaac cacttttgcc aacttctagc ctgtcgattg
     3361 ttaacaggtc caaggaagag gtcacatcac ataaatatca gcacgaaaaa ttatttattc
     3421 aagtgaaagg gaagaaaagt cgtcatcaaa aagaggattc cctttcttgg agtaatagtg
     3481 cttatttatc cttggatgat gatgctttca cggctccatt tcatagggat ggaatgctga
     3541 gtttaaagca actactgtct tttctcagta tcacagactt tcagttacct gatgaagact
     3601 ttggacctct taagcttgaa aaagtgaagt cctgctcaga aaaaccagtg gagccctttg
     3661 agtcaaaaat gtttggagag agacatctta aagagggaag ctgtattttt ccagaggaac
     3721 tgagtcctaa acgcatggat acagaaatgg aggacttaga agaggacctt attgttctac
     3781 caggaaaatc acatcccaaa aggccaaact cgcaaagcca gcatacaaag acgggccttt
     3841 cttcatccat attactttat actcctttaa atacggttgc gcctgatgat aatgacaggc
     3901 ctaccacaga catgtgttca cctgctttcc ccatcttagg tactactcca gcctttggcc
     3961 ctcaaggctc ctatgaaaaa gcatctacag aagttgctgg acgaacttgc tgcacacccc
     4021 aacttgctca tttgaaagac tcagtctgtc ttgccagtga tactaaacaa ttcgacagtt
     4081 caggcagccc agcaaaacca cataccaccc tgcaagtgtc aggcaggcaa ggacaaccta
     4141 cctgtgactg tgactctgtc ccgccaggaa cacctccacc cattgagtca ttcactttta
     4201 aagaaaatca gctctgtaga aacacatgcc aggagctgca taaacattcc gtcgaacaga
     4261 ctgaaacagc agagcttcct gcttctgata gcataaaccc aggcaaccta caattggttt
     4321 cagagttaaa gaatccttca ggttcctgtt ccgtagatgt gagtgccatg ttttgggaaa
     4381 gagccggttg taaagagcca tgtatcataa ctgcttgcga agatgtagtt tctctttgga
     4441 aagctctgga tgcttggcag tgggaaaaac tttatacctg gcacttcgca gaggttccag
     4501 tattacagat agttccagtg cctgatgtgt ataatctcgt gtgtgtagct ttgggaaatt
     4561 tggaaatcag agagatcagg gcattgtttt gttcctctga tgatgaaagt gaaaagcaag
     4621 tactactgaa gtctggaaat ataaaagctg tgcttggcct gacaaagagg aggctagtta
     4681 gtagcagtgg gaccctttct gatcaacaag tagaagtcat gacgtttgca gaagatggag
     4741 gaggcaaaga aaaccaattt ttgatgcccc ctgaggagac tatactaact tttgctgagg
     4801 tccaagggat gcaagaagct ctgcttggta ctactattat gaacaacatt gttatttgga
     4861 atttaaaaac tggtcaactc ctgaaaaaga tgcacattga tgattcttac caagcttcag
     4921 tctgtcacaa agcctattct gaaatggggc ttctctttat tgtcctgagt catccctgtg
     4981 ccaaagagag tgagtcgttg cgaagccctg tgtttcagct cattgtgatt aaccctaaga
     5041 cgactctcag cgtgggtgtg atgctgtact gtcttcctcc agggcaggct ggcaggttcc
     5101 tggaaggtga cgtgaaagat cactgtgcag cagcaatctt gacttctgga acaattgcca
     5161 tttgggactt acttctcggt cagtgtactg ccctcctccc acctgtctct gaccaacatt
     5221 ggtcttttgt gaaatggtcg ggtacagact ctcatttgct ggctggacaa aaagatggaa
     5281 atatatttgt ataccactat tcataaatag tgactggata tgttgtgttt tacagtatta
     5341 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     5401 ttctcgttca gctttcttgt acaaagtggt gtgactcgag tctagagggc ccgtttaaac
     5461 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5521 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5581 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5641 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5701 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     5761 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5821 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5881 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5941 cctcgacccc aaaaaacttg attagggtga tggttcacgt acctagaagt tcctattccg
     6001 aagttcctat tctctagaaa gtataggaac ttccttggcc aaaaagcctg aactcaccgc
     6061 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     6121 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     6181 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     6241 atcggccgcg ctcccgattc cggaagtgct tgacattggg gatttcagcg agagcctgac
     6301 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     6361 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     6421 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     6481 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     6541 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     6601 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     6661 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     6721 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     6781 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     6841 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     6901 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     6961 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     7021 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt
     7081 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     7141 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     7201 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7261 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7321 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     7381 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7441 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7501 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7561 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7621 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7681 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7741 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7801 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7861 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7921 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7981 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8041 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8101 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8161 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8221 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8281 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8341 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8401 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8461 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8521 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8581 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8641 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8701 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8761 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8821 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8881 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8941 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9001 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9061 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9121 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9181 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9241 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9301 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9361 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9421 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9481 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
