LOCUS       sequence-193377-        9454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expession of GFP-PALB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-FRT/T0-GFP-PALB2
FEATURES             Location/Qualifiers
     source          1..9454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(990..992,993..995,996..1706)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(990..992,993..995,996..1706)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1035..1056)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1296..1315)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1643..1664
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1716..1740
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5306..5330)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5330..5354)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5406..5423)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5412..5636
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5769..5788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5920..5967
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5975..6997
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7127..7248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7164..7183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7218..7237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7297..7313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7297..7313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7310..7332)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7321..7337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7345..7351,7352..7369,7370..7375))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7390..7411
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7528..7545)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7699..8287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7779..7798)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8458..9249,9250..9318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9081..9100
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9319..9423)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggtgagcaa gggcgaggag ctgttcaccg
     1021 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1081 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1141 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1201 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1261 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1321 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1381 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1441 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1501 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1561 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1621 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1681 ctctcggcat ggacgagctg tacaagggcg cgcccacaag tttgtacaaa aaagcaggct
     1741 ccaccatgga cgagcctccc gggaagcccc tcagctgtga ggagaaggaa aagttaaagg
     1801 agaaattagc attcttgaaa agggaataca gcaagacact agcccgcctt cagcgtgccc
     1861 aaagagctga aaagattaag cattctatta agaaaacagt agaagaacaa gattgtttgt
     1921 ctcagcagga tctctcaccg cagctaaaac actcagaacc taaaaataaa atatgtgttt
     1981 atgacaagtt acacatcaaa acccatcttg atgaagaaac tggagaaaag acatctatca
     2041 cacttgatgt tgggcctgag tcctttaacc ctggagatgg cccaggagga ttacctatac
     2101 aaagaacaga tgacacccaa gaacattttc cccacagggt cagtgaccct agtggtgagc
     2161 aaaagcagaa gctgccaagc agaagaaaga agcagcagaa gaggacattt atttcacagg
     2221 agagagactg tgtctttggc actgattcac tcagattgtc tgggaaaaga ctaaaggaac
     2281 aggaagaaat cagtagcaaa aatcctgcta gatcaccagt aactgaaata agaactcacc
     2341 ttttaagtct taaatctgaa cttccagatt ctccagaacc agttacagaa attaatgaag
     2401 acagtgtatt aattccacca actgcccaac cagaaaaagg tgttgataca ttcctaagaa
     2461 gacctaattt caccagggcg actacagttc ctttacagac tctatcagat agcggtagta
     2521 gtcagcacct tgaacacatt cctcctaaag gtagcagtga acttactact cacgacctaa
     2581 aaaacattag atttacttca cctgtaagtt tggaggcaca aggcaaaaaa atgactgtct
     2641 ctacagataa cctccttgta aataaagcta taagtaaaag tggccaactg cccacaagtt
     2701 ctaatttaga ggcaaatatt tcatgttctc taaatgaact cacctacaat aacttaccag
     2761 caaatgaaaa ccaaaactta aaagaacaaa atcaaacaga gaaatcttta aaatctccca
     2821 gtgacactct tgatggcagg aatgaaaatc ttcaggaaag tgagattcta agtcaaccta
     2881 agagtcttag cctggaagca acctctcctc tttctgcaga aaaacattct tgcacagtgc
     2941 ctgaaggcct tctgtttcct gcagaatatt atgttagaac aacacgaagc atgtccaatt
     3001 gccagaggaa agtagccgtg gaggctgtca ttcagagtca tttggatgtc aagaaaaaag
     3061 ggtttaaaaa taaaaataag gatgcaagta aaaatttaaa cctttccaat gaggaaactg
     3121 accaaagtga aattaggatg tctggcacat gcacaggaca accaagttca agaacctctc
     3181 agaaacttct ctcattaact aaagtcagct ctcccgctgg gcccactgaa gataatgact
     3241 tgtctaggaa ggcagttgcc caagcacctg gtagaagata cacaggaaaa agaaaatcag
     3301 cctgcacccc agcatcagat cattgtgaac cacttttgcc aacttctagc ctgtcgattg
     3361 ttaacaggtc caaggaagag gtcacatcac ataaatatca gcacgaaaaa ttatttattc
     3421 aagtgaaagg gaagaaaagt cgtcatcaaa aagaggattc cctttcttgg agtaatagtg
     3481 cttatttatc cttggatgat gatgctttca cggctccatt tcatagggat ggaatgctga
     3541 gtttaaagca actactgtct tttctcagta tcacagactt tcagttacct gatgaagact
     3601 ttggacctct taagcttgaa aaagtgaagt cctgctcaga aaaaccagtg gagccctttg
     3661 agtcaaaaat gtttggagag agacatctta aagagggaag ctgtattttt ccagaggaac
     3721 tgagtcctaa acgcatggat acagaaatgg aggacttaga agaggacctt attgttctac
     3781 caggaaaatc acatcccaaa aggccaaact cgcaaagcca gcatacaaag acgggccttt
     3841 cttcatccat attactttat actcctttaa atacggttgc gcctgatgat aatgacaggc
     3901 ctaccacaga catgtgttca cctgctttcc ccatcttagg tactactcca gcctttggcc
     3961 ctcaaggctc ctatgaaaaa gcatctacag aagttgctgg acgaacttgc tgcacacccc
     4021 aacttgctca tttgaaagac tcagtctgtc ttgccagtga tactaaacaa ttcgacagtt
     4081 caggcagccc agcaaaacca cataccaccc tgcaagtgtc aggcaggcaa ggacaaccta
     4141 cctgtgactg tgactctgtc ccgccaggaa cacctccacc cattgagtca ttcactttta
     4201 aagaaaatca gctctgtaga aacacatgcc aggagctgca taaacattcc gtcgaacaga
     4261 ctgaaacagc agagcttcct gcttctgata gcataaaccc aggcaaccta caattggttt
     4321 cagagttaaa gaatccttca ggttcctgtt ccgtagatgt gagtgccatg ttttgggaaa
     4381 gagccggttg taaagagcca tgtatcataa ctgcttgcga agatgtagtt tctctttgga
     4441 aagctctgga tgcttggcag tgggaaaaac tttatacctg gcacttcgca gaggttccag
     4501 tattacagat agttccagtg cctgatgtgt ataatctcgt gtgtgtagct ttgggaaatt
     4561 tggaaatcag agagatcagg gcattgtttt gttcctctga tgatgaaagt gaaaagcaag
     4621 tactactgaa gtctggaaat ataaaagctg tgcttggcct gacaaagagg aggctagtta
     4681 gtagcagtgg gaccctttct gatcaacaag tagaagtcat gacgtttgca gaagatggag
     4741 gaggcaaaga aaaccaattt ttgatgcccc ctgaggagac tatactaact tttgctgagg
     4801 tccaagggat gcaagaagct ctgcttggta ctactattat gaacaacatt gttatttgga
     4861 atttaaaaac tggtcaactc ctgaaaaaga tgcacattga tgattcttac caagcttcag
     4921 tctgtcacaa agcctattct gaaatggggc ttctctttat tgtcctgagt catccctgtg
     4981 ccaaagagag tgagtcgttg cgaagccctg tgtttcagct cattgtgatt aaccctaaga
     5041 cgactctcag cgtgggtgtg atgctgtact gtcttcctcc agggcaggct ggcaggttcc
     5101 tggaaggtga cgtgaaagat cactgtgcag cagcaatctt gacttctgga acaattgcca
     5161 tttgggactt acttctcggt cagtgtactg ccctcctccc acctgtctct gaccaacatt
     5221 ggtcttttgt gaaatggtcg ggtacagact ctcatttgct ggctggacaa aaagatggaa
     5281 atatatttgt ataccactat tcataaaccc gctttcttgt acaaagtggt ttcagctttc
     5341 ttgtacaaag tggtgtgact cgagctgact cgagtctaga gggcccgttt aaacccgctg
     5401 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5461 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5521 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5581 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5641 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     5701 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5761 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5821 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5881 ccccaaaaaa cttgattagg gtgatggttc acgtacctag aagttcctat tccgaagttc
     5941 ctattctcta gaaagtatag gaacttcctt ggccaaaaag cctgaactca ccgcgacgtc
     6001 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     6061 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     6121 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     6181 cgcgctcccg attccggaag tgcttgacat tggggatttc agcgagagcc tgacctattg
     6241 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     6301 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     6361 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     6421 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     6481 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     6541 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     6601 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     6661 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     6721 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     6781 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     6841 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     6901 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6961 ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtactacgag atttcgattc
     7021 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     7081 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     7141 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7201 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     7261 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7321 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7381 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7441 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7501 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7561 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7621 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7681 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7741 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7801 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7861 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7921 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7981 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8041 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8101 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8161 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8221 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8281 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8341 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8401 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8461 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8521 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8581 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8641 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8701 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8761 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8821 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8881 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8941 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9001 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9061 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9121 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9181 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9241 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9301 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9361 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9421 gcgcacattt ccccgaaaag tgccacctga cgtc
//
