LOCUS       sequence-19339-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf268
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             268..309
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             319..336
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(359..376)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    365..589
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 accgagctcg gatccagtac ccttcaccat gataagtaat tacgagccgt tgctgctgtt
       61 agttataaca tgctgtgtac tactatttaa ttttaccata tcttcgaaaa caaaaataga
      121 tattattttt gcagtacaaa ctattgtttt tatatggttt atattccact ttgttcattc
      181 ggcgatttaa ggatcaaggg tcaagacaat tctgcagata tccagcacag tggcggccgc
      241 tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
      301 gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
      361 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
      421 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
      481 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
      541 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
      601 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
      661 gcggcgggtg tggtggttac gcgcagcgtg accgctacac tttgcagcgc cctagcgccc
      721 gctccttt
//
