LOCUS       sequence-193397-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 
            chimera that enables optical control of calcium signals and calcium 
            dependent signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   He L, Zhang Y, Ma G, Tan P, Li Z, Zang S, Wu X, Jing J, Fang S, Zhou
            L, Wang Y, Huang Y, Hogan PG, Han G, Zhou Y
  TITLE     Near-infrared photoactivatable control of Ca(2+) signaling and 
            optogenetic immunomodulation.
  JOURNAL   Elife. 2015 Dec 8;4. pii: e10024. doi: 10.7554/eLife.10024.
  PUBMED    26646180
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-8"; volume: "4. pii: e10024. doi"; pages: " 
            10.7554/eLife.10024"
COMMENT     Sequence Label: Opto-CRAC version 1
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..429
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 ttggctacta cacttgaacg tattgagaag aactttgtca ttactgaccc aagattgcca
       61 gataatccca ttatattcgc gtccgatagt ttcttgcagt tgacagaata tagccgtgaa
      121 gaaattttgg gaagaaactg caggtttcta caaggtcctg aaactgatcg cgcgacagtg
      181 agaaaaatta gagatgccat agataaccaa acagaggtca ctgttcagct gattaattat
      241 acaaagagtg gtaaaaagtt ctggaacctc tttcacttgc agcctatgcg agatcagaag
      301 ggagatgtcc agtactttat tggggttcag ttggatggaa ctgagcatgt ccgagatgct
      361 gccgagagag agggagtcat gctgattaag aaaactgcag aaaatattga tgaggcggca
      421 aaagaactta agcttgaatc acacagctca tggtatgctc ctgaggccct gcagaagtgg
      481 ctgcagctga cccatgaggt ggaggtgcag tactacaaca tcaagaagca aaatgcagag
      541 aggcagctgc tggtggccaa ggagggggct gagaaaataa aaaagaagag aaacacgctt
      601 tttggtacct tccatgtggc ccacagctct tccctggatg atgtggatca taaaatccta
      661 actgctaagc aagctctgag tgaggtgaca gcggcactga gggagcgcct gcaccggtgg
      721 cagcagatcg agatcctctg cggtttccag attgtcaata accccggcat ccactccttg
      781 gtggctgctc tcaacatcga ccccagctgg atgggcagca cccgccctaa ccccgcccac
      841 ttcatcatga ctgacgatgt ggatgacatg gatgaggaga ttgtgtcgta a
//
