LOCUS       sequence-193400-        5663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5663)
  TITLE     SGC Oxford 2017
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: JAK3 (JAK3A-c023)
FEATURES             Location/Qualifiers
     source          1..5663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     polyA_signal    1269..1403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1306..1325)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1360..1379
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1432..1597
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1781..2235
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1868..1887)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2077..2098
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2261..2365
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2366..2434,2435..3226)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2584..2603)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3397..3985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3886..3905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4139..4156
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4290..4514
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4581..5114)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5284..5289,5290..5306,5307..5312))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg ggccaccatc atcatcatca ttcttctggt
      181 gtagatctgg gtaccgagaa cctgtacttc caatccatgc aagaccccac gatcttcgag
      241 gagagacacc tcaagtacat ctcacagctg ggcaagggca actttggcag cgtggagctg
      301 tgccgctatg acccgctagg cgacaataca ggtgccctgg tggccgtgaa acagctgcag
      361 cacagcgggc cagaccagca gagggacttt cagcgggaga ttcagatcct caaagcactg
      421 cacagtgatt tcattgtcaa gtatcgtggt gtcagctatg gcccgggccg ccagagcctg
      481 cggctggtca tggagtacct gcccagcggc tgcttgcgcg acttcctgca gcggcaccgc
      541 gcgcgcctcg atgccagccg cctccttctc tattcctcgc agatctgcaa gggcatggag
      601 tacctgggct cccgccgctg cgtgcaccgc gctctggccg cccgaaacat cctcgtggag
      661 agcgaggcac acgtcaagat cgctgacttc ggcctagcta agctgctgcc gcttgacaaa
      721 gactactacg tggtccgcga gccaggccag agccccattt tctggtatgc ccccgaatcc
      781 ctctcggaca acatcttctc tcgccagtca gacgtctgga gcttcggggt cgtcctgtac
      841 gagctcttca cctactgcga caaaagctgc agcccctcgg ccgagttcct gcggatgatg
      901 ggatcagagc gggatgtccc cgccctcagc cgcctcttgg aactgctgga ggagggccag
      961 aggctgccgg cgcctcctgc ctgccctgct gaggttcacg agctcatgaa gctgtgctgg
     1021 gcccctagcc cacaggaccg gccatcattc agcgccctgg gcccccagct ggacatgctg
     1081 tggagcggaa gccggtgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca
     1141 agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac
     1201 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     1261 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1321 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1381 atgtatctta tcatgtctgg atctgatcac tgatatcgcc taggagatcc gaaccagata
     1441 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     1501 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
     1561 cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt cttcctgtta
     1621 tgtttttaat caaacatcct gccaactcca tgtgacaaac cgtcatcttc ggctactttt
     1681 tctctgtcac agaatgaaaa tttttctgtc atctcttcgt tattaatgtt tgtaattgac
     1741 tgaatatcaa cgcttatttg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     1801 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     1861 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     1921 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     1981 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2041 tttcgccctt tgacgttgga gtccacgttc ttaatagtgg actcttgttc caaactggaa
     2101 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2161 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     2221 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2281 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2341 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2401 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2461 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2521 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2581 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2641 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2701 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2761 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2821 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2881 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2941 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3001 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3061 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3121 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3181 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3241 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3301 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3361 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3421 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3481 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3541 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3601 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3661 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3721 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3781 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3841 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3901 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3961 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4021 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4081 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4141 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     4201 gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg gcaaaatcgg ttacggttga
     4261 gtaataaatg gatgccctgc gtaagcgggt gtgggcggac aataaagtct taaactgaac
     4321 aaaatagatc taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa
     4381 atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc
     4441 ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg
     4501 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     4561 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     4621 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     4681 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgagcag attgatgagc
     4741 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     4801 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     4861 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     4921 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     4981 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     5041 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     5101 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     5161 ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     5221 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     5281 tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac
     5341 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
     5401 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
     5461 ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg
     5521 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
     5581 ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta
     5641 cgtatactcc ggaatattaa tag
//
