LOCUS       sequence-193408-       11358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for N-terminal HA-tagged human CCR5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E
  TITLE     Mapping Interaction Sites on Human Chemokine Receptors by Deep 
            Mutational Scanning.
  JOURNAL   J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 
            10.4049/jimmunol.1800343.
  PUBMED    29678950
REFERENCE   2  (bases 1 to 11358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343."
COMMENT     Sequence Label: pCEP4-HA-CCR5
FEATURES             Location/Qualifiers
     source          1..11358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      475..2264
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2570..4495)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(4930..4948)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5016..5120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5121..5189,5190..5981)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5339..5358)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6152..6740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6641..6660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7171..7316
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7359..8396
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(8394..8413)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8438..8486
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        9140..9519
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9520..9723
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9674..9694
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9720..9744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             9826..9852
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             9826..9852
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    10018..10029
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    10949..11083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10973..10992)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11027..11046
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(11225..11247)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gccccgccgc cggacgaact aaacctgact acggcatctc tgccccttct tcgctggtac
       61 gaggagcgct tttgttttgt attcggggca gtgcatgtaa tcccttcagt tggttggtac
      121 aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac acaaaggggt
      181 tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac actgagtggc
      241 tttcatcctg gagcagactt tgcatgctgt ggactgcaac acaacattgc ctttatgtgt
      301 aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg
      361 aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg
      421 gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg
      481 tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat
      541 atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa
      601 cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca
      661 cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt
      721 gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact
      781 gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc
      841 ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata
      901 accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct
      961 ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac
     1021 gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga
     1081 tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag
     1141 gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa
     1201 aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt
     1261 tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac
     1321 tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac
     1381 cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg
     1441 gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga
     1501 gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct
     1561 aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     1621 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     1681 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     1741 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     1801 tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt
     1861 tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     1921 tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     1981 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     2041 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     2101 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgcata
     2161 tacagtcagc atatgatacc cagtagtaga gtgggagtgc tatcctttgc atatgccgcc
     2221 acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt cctgcatgct ggttgctccc
     2281 attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat tgctcaccag
     2341 gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct gagtgacgtg
     2401 acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga caagacagat
     2461 ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct ttagtgcggg
     2521 ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat cactcctgcc
     2581 cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca tcaccctccg
     2641 cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca tcgtcaaagc
     2701 tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca aggtccttaa
     2761 tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg aaataacaga
     2821 caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt ccaaagggga
     2881 gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct cgccttaggt
     2941 tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa gttccttcgt
     3001 cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct gcaatgttct
     3061 caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct ccttttttgc
     3121 gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct gggtcatctg
     3181 cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg ccaccttctt
     3241 ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt ctacctggag
     3301 ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc ctgggcctct
     3361 tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc ctggctcttt
     3421 cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca ctacctcctc
     3481 gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct gcccctcctg
     3541 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc
     3601 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctcctgctc
     3661 ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     3721 ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gctcctgccc
     3781 ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc
     3841 ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgctcct gcccctcctc
     3901 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     3961 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg
     4021 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     4081 cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     4141 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgctc
     4201 ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg cagccaatgc
     4261 aacttggacg tttttggggt ctccggacac catctctatg tcttggccct gatcctgagc
     4321 cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat
     4381 ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt ccagatgtgt
     4441 ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca gacatgattc
     4501 acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata cagggagtgc
     4561 agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac
     4621 agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact
     4681 cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag
     4741 gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc
     4801 tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg
     4861 ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa
     4921 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4981 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5041 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5101 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5161 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5221 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5281 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5341 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     5401 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5461 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5521 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5581 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5641 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     5701 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5761 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5821 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5881 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5941 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6001 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6061 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6781 gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     6841 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     6901 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt
     6961 gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt
     7021 cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag
     7081 gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa
     7141 gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca
     7201 ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat
     7261 attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc
     7321 gtcaacagcg tgccgcagat cccggggggc aatgagatat gaaaaagcct gaactcaccg
     7381 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     7441 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     7501 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     7561 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     7621 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     7681 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     7741 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     7801 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     7861 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     7921 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     7981 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     8041 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     8101 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
     8161 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     8221 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     8281 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     8341 gtggaaaccg acgccccagc actcgtccgg atcgggagat gggggaggct aactgaaaca
     8401 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     8461 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     8521 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     8581 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     8641 ccctgccata gccactggcc ccgtgggtta gggacggggt cccccatggg gaatggttta
     8701 tggttcgtgg gggttattat tttgggcgtt gcgtggggtc aggtccacga ctggactgag
     8761 cagacagacc catggttttt ggatggcctg ggcatggacc gcatgtactg gcgcgacacg
     8821 aacaccgggc gtctgtggct gccaaacacc cccgaccccc aaaaaccacc gcgcggattt
     8881 ctggcgtgcc aagctagtcg accaattctc atgtttgaca gcttatcatc gcagatccgg
     8941 gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg
     9001 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
     9061 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
     9121 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
     9181 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     9241 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     9301 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     9361 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     9421 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     9481 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     9541 accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     9601 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac
     9661 cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     9721 gctcgtttag tgaaccgtca gatctctaga agctgggtac cagctgctag cgccaccatg
     9781 aagaccatca tcgccctgag ctacatcttc tgcctggtgt tcgcctaccc ctacgacgtg
     9841 cccgactacg ccggctcccc gggaggcgcg gattatcaag tgtcaagtcc aatctatgac
     9901 atcaattatt atacatcgga gccctgccaa aaaatcaatg tgaagcaaat cgcagcccgc
     9961 ctcctgcctc cgctctactc actggtgttc atctttggtt ttgtgggcaa catgctggtc
    10021 atcctcatcc tgataaactg caaaaggctg aagagcatga ctgacatcta cctgctcaac
    10081 ctggccatct ctgacctgtt tttccttctt actgtcccct tctgggctca ctatgctgcc
    10141 gcccagtggg actttggaaa tacaatgtgt caactcttga cagggctcta ttttataggc
    10201 ttcttctctg gaatcttctt catcatcctc ctgacaatcg ataggtacct ggctgtcgtc
    10261 catgctgtgt ttgctttaaa agccaggacg gtcacctttg gggtggtgac aagtgtgatc
    10321 acttgggtgg tggctgtgtt tgcgtctctc ccaggaatca tctttaccag atctcaaaaa
    10381 gaaggtcttc attacacctg cagctctcat tttccataca gtcagtatca attctggaag
    10441 aatttccaga cattaaagat agtcatcttg gggctggtcc tgccgctgct tgtcatggtc
    10501 atctgctact cgggaatcct aaaaactctg cttcggtgtc gaaatgagaa gaagaggcac
    10561 agggctgtga ggcttatctt caccatcatg attgtttatt ttctcttctg ggctccctac
    10621 aacattgtcc ttctcctgaa caccttccag gaattctttg gcctgaataa ttgcagtagc
    10681 tctaacaggt tggaccaagc tatgcaggtg acagagactc ttgggatgac gcactgctgc
    10741 atcaacccca tcatctatgc ctttgtcggg gagaagttca gaaactacct cttagtcttc
    10801 ttccaaaagc acattgccaa acgcttctgc aaatgctgtt ctattttcca gcaagaggct
    10861 cccgagcgag caagctcagt ttacacccga tccactgggg agcaggaaat atctgtgggc
    10921 ttgtgataac tcgaggccgg caaggccgga tccagacatg ataagataca ttgatgagtt
    10981 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
    11041 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
    11101 tcattttatg tttcaggttc agggggaggt ggggaggttt tttaaagcaa gtaaaacctc
    11161 tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg
    11221 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    11281 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
    11341 tgaccggtcg actctaga
//
