LOCUS       sequence-19343-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Kaper T, Looger LL, Takanaga H, Platten M, Steinman L, Frommer WB
  TITLE     Nanosensor detection of an immunoregulatory tryptophan 
            influx/kynurenine efflux cycle.
  JOURNAL   PLoS Biol. 2007 Oct;5(10):e257.
  PUBMED    17896864
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2007-10"; volume: "5(10)"; pages: "e257"
COMMENT     Sequence Label: pTK222
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(143..145,146..148,149..607)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     complement(188..209)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(449..468)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     gap             681..696
                     /estimated_length=16
ORIGIN
        1 cgtggngnnt tagcagagct ctctggctac tagagaaccc actgcttact ggcttatcga
       61 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt
      121 accgagctcg gatccgggcc gcatggtgag caagggcgag gagctgttca ccggggtggt
      181 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      241 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      301 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag
      361 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      421 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      481 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      541 ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat
      601 caccgccgac aagcagaaga acggcatcaa ggccaacttc aaaggannca cggaggtgtc
      661 gacnnaaaac nnaaanaaaa nnnnnnnnnn nnnnnngggn nngtntnttt tccaaaannt
      721 ttnaccttcc naaaaaacaa aaaaanaaaa ngttgttntg tcccgggtng accccgnntt
      781 anaaattgaa aaangtccca atcctttccn ngaancccng gtttttcctt aaaccaaatn
      841 ttttt
//
