LOCUS       sequence-193441-       11106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one CRISPR plasmid for integrating, deleting, or replacing a 
            gene in S. cerevisiae. High-copy, URA3 plasmid contains human 
            codon-optimized Cas9 and a guide RNA targeting ARS1206a sequence 
            CGAACATTTTTCCATGCGCT in yeast chromosome 12..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Reider Apel A, d'Espaux L, Wehrs M, Sachs D, Li RA, Tong GJ, Garber 
            M, Nnadi O, Zhuang W, Hillson NJ, Keasling JD, Mukhopadhyay A
  TITLE     A Cas9-based toolkit to program gene expression in Saccharomyces 
            cerevisiae.
  JOURNAL   Nucleic Acids Res. 2017 Jan 9;45(1):496-508. doi: 
            10.1093/nar/gkw1023. Epub 2016 Nov 28.
  PUBMED    27899650
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw1023"; journalName: "Nucleic Acids Res"; date: 
            "2017-01-9- 9"; volume: "45"; issue: "1"; pages: "496-508"
COMMENT     Sequence Label: p426_Cas9_gRNA-ARS1206a
FEATURES             Location/Qualifiers
     source          1..11106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(111..326)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(350..369)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     469..491
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(529..547)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      588..1930
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    920..967
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1957..2061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2062..2130,2131..2922)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2280..2299)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3093..3681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3582..3601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3835..3852
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3969..3990
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4005..4010,4011..4028,4029..4035)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4043..4059
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4048..4070
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4067..4083
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4067..4083
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4102..4122
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4104..4122
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_RNA        4349..4424
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        4510..5214
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5224..9327
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKNERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIYLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9340..9360
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      9376..9623
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(9641..9660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9642..9660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9670..9687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9670..9686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9679..9701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9827..10282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9914..9933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10124..10145
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(10413..11106,1..110))
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca
       61 acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt
      121 cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt
      181 tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct
      241 tccttctgtt cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa
      301 gaataaaaaa aaaatgatga attgaaaagg tggtatggtg cactctcagt acaatctgct
      361 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
      421 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
      481 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
      541 gcctattttt ataggttaat gtcatgataa taatggtttc ttagtatgat ccaatatcaa
      601 aggaaatgat agcattgaag gatgagacta atccaattga ggagtggcag catatagaac
      661 agctaaaggg tagtgctgaa ggaagcatac gataccccgc atggaatggg ataatatcac
      721 aggaggtact agactacctt tcatcctaca taaatagacg catataagta cgcatttaag
      781 cataaacacg cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata
      841 taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc
      901 tcgttttcgg aaacgctttg aagttcctat tccgaagttc ctattctcta gaaagtatag
      961 gaacttcaga gcgcttttga aaaccaaaag cgctctgaag acgcactttc aaaaaaccaa
     1021 aaacgcaccg gactgtaacg agctactaaa atattgcgaa taccgcttcc acaaacattg
     1081 ctcaaaagta tctctttgct atatatctct gtgctatatc cctatataac ctacccatcc
     1141 acctttcgct ccttgaactt gcatctaaac tcgacctcta cattttttat gtttatctct
     1201 agtattactc tttagacaaa aaaattgtag taagaactat tcatagagtg aatcgaaaac
     1261 aatacgaaaa tgtaaacatt tcctatacgt agtatataga gacaaaatag aagaaaccgt
     1321 tcataatttt ctgaccaatg aagaatcatc aacgctatca ctttctgttc acaaagtatg
     1381 cgcaatccac atcggtatag aatataatcg gggatgcctt tatcttgaaa aaatgcaccc
     1441 gcagcttcgc tagtaatcag taaacgcggg aagtggagtc aggctttttt tatggaagag
     1501 aaaatagaca ccaaagtagc cttcttctaa ccttaacgga cctacagtgc aaaaagttat
     1561 caagagactg cattatagag cgcacaaagg agaaaaaaag taatctaaga tgctttgtta
     1621 gaaaaatagc gctctcggga tgcatttttg tagaacaaaa aagaagtata gattctttgt
     1681 tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt
     1741 gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta caaaaatgaa gcacagattc
     1801 ttcgttggta aaatagcgct ttcgcgttgc atttctgttc tgtaaaaatg cagctcagat
     1861 tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag
     1921 atgcttcgtt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1981 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2041 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2101 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2161 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2221 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2281 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2341 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2401 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2461 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2521 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2581 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2641 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2701 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2761 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2821 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2881 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2941 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3001 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3061 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3121 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3181 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3241 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3301 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3361 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3421 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3481 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3541 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3601 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3661 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3721 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3781 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3841 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3901 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3961 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     4021 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     4081 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagcta
     4141 gtatactctt tcttcaacaa ttaaatactc tcggtagcca agttggttta aggcgcaaga
     4201 ctgtaattta tcactacgaa atcttgagat cgggcgttcg actcgccccc gggagagatg
     4261 gccggcatgg tcccagcctc ctcgctggcg ccggctgggc aacaccttcg ggtggcgaat
     4321 gggactttcg aacatttttc catgcgctgt tttagagcta gaaatagcaa gttaaaataa
     4381 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tattttttgt
     4441 cactattgtt atgtaaaatg ccacctctga cagtatggaa cgcaaacttc tgtctagtgg
     4501 atagtcgaca tccttttgtt gtttccgggt gtacaatatg gacttcctct tttctggcaa
     4561 ccaaacccat acatcgggat tcctataata ccttcgttgg tctccctaac atgtaggtgg
     4621 cggaggggag atatacaata gaacagatac cagacaagac ataatgggct aaacaagact
     4681 acaccaatta cactgcctca ttgatggtgg tacataacga actaatactg tagccctaga
     4741 cttgatagcc atcatcatat cgaagtttca ctaccctttt tccatttgcc atctattgaa
     4801 gtaataatag gcgcatgcaa cttcttttct ttttttttct tttctctctc ccccgttgtt
     4861 gtctcaccat atccgcaatg acaaaaaaat gatggaagac actaaaggaa aaaattaacg
     4921 acaaagacag caccaacaga tgtcgttgtt ccagagctga tgaggggtat ctcgaagcac
     4981 acgaaacttt ttccttcctt cattcacgca cactactctc taatgagcaa cggtatacgg
     5041 ccttccttcc agttacttga atttgaaata aaaaaaagtt tgctgtcttg ctatcaagta
     5101 taaatagacc tgcaattatt aatcttttgt ttcctcgtca ttgttctcgt tccctttctt
     5161 ccttgtttct ttttctgcac aatatttcaa gctataccaa gcatacaatc aacttggaaa
     5221 aaaatggaca agaagtactc cattgggctc gatatcggca caaacagcgt cggttgggcc
     5281 gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat
     5341 cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc
     5401 gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc
     5461 tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat
     5521 aggctggagg agtccttttt ggtggaggag gataaaaaga acgagcgcca cccaatcttt
     5581 ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg
     5641 aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg
     5701 catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc
     5761 gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac
     5821 ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc
     5881 cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt
     5941 aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc
     6001 gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg
     6061 gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc
     6121 attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct
     6181 agtatgatca agcgctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc
     6241 agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac
     6301 gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc
     6361 ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga agatctgttg
     6421 cgcaaacagc gcactttcga caatggaagc atcccccacc agattcacct gggcgaactg
     6481 cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag
     6541 attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc ccggggaaat
     6601 tccagattcg cgtggatgac tcgcaaatca gaagagacca tcactccctg gaacttcgag
     6661 gaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat
     6721 aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca
     6781 gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc
     6841 ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt
     6901 accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga ctctgttgaa
     6961 atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa
     7021 atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt
     7081 gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac
     7141 gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata tacaggatgg
     7201 gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg aaagacaatc
     7261 ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat
     7321 gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt
     7381 cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag
     7441 accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc
     7501 gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa
     7561 aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac
     7621 ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc
     7681 agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggat
     7741 catatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt gttgacaaga
     7801 tccgataaaa atagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg
     7861 aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat
     7921 ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg
     7981 cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg
     8041 aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag
     8101 tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac
     8161 aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa
     8221 aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg
     8281 aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac
     8341 agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag
     8401 cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat
     8461 ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa
     8521 gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg
     8581 atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc
     8641 gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc
     8701 gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc
     8761 gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc
     8821 aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag
     8881 ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc
     8941 agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca gctgttcgtg
     9001 gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga
     9061 gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat
     9121 aagcccatca gggagcaggc agaaaacatt atctacttgt ttactctgac caacttgggc
     9181 gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta cacctctaca
     9241 aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga
     9301 atcgacctct ctcagctcgg tggagacagc agggctgacc ccaagaagaa gaggaaggtg
     9361 tgatctcttc tcgagtcatg taattagtta tgtcacgctt acgttcacgc cctcccccca
     9421 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt
     9481 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc
     9541 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg
     9601 gacgctcgaa ggctttaatt tgcggccggt acccaattcg ccctatagtg agtcgtatta
     9661 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     9721 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     9781 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc
     9841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     9901 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     9961 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    10021 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    10081 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10141 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    10201 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    10261 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
    10321 tttcacaccg catagggtaa taactgatat aattaaattg aagctctaat ttgtgagttt
    10381 agtatacatg catttactta taatacagtt ttttagtttt gctggccgca tcttctcaaa
    10441 tatgcttccc agcctgcttt tctgtaacgt tcaccctcta ccttagcatc ccttcccttt
    10501 gcaaatagtc ctcttccaac aataataatg tcagatcctg tagagaccac atcatccacg
    10561 gttctatact gttgacccaa tgcgtctccc ttgtcatcta aacccacacc gggtgtcata
    10621 atcaaccaat cgtaaccttc atctcttcca cccatgtctc tttgagcaat aaagccgata
    10681 acaaaatctt tgtcgctctt cgcaatgtca acagtaccct tagtatattc tccagtagat
    10741 agggagccct tgcatgacaa ttctgctaac atcaaaaggc ctctaggttc ctttgttact
    10801 tcttctgccg cctgcttcaa accgctaaca atacctgggc ccaccacacc gtgtgcattc
    10861 gtaatgtctg cccattctgc tattctgtat acacccgcag agtactgcaa tttgactgta
    10921 ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa aattgtactt ggcggataat
    10981 gcctttagcg gcttaactgt gccctccatg gaaaaatcag tcaagatatc cacatgtgtt
    11041 tttagtaaac aaattttggg acctaatgct tcaactaact ccagtaattc cttggtggta
    11101 cgaaca
//
