LOCUS       sequence-193452-        4442 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-dL5(E52D)-BKa (KCNMA1, mSlo alpha subunit) in mammalian
            cells, with an Igk-leader. (MBIC5, dL5**, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Pratt CP, He J, Wang Y, Barth AL, Bruchez MP
  TITLE     Fluorogenic Green-Inside Red-Outside (GIRO) Labeling Approach 
            Reveals Adenylyl Cyclase-Dependent Control of BKalpha Surface 
            Expression.
  JOURNAL   Bioconjug Chem. 2015 Sep 16;26(9):1963-71. doi: 
            10.1021/acs.bioconjchem.5b00409. Epub 2015 Sep 2.
  PUBMED    26301573
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.bioconjchem.5b00409"; journalName: "Bioconjug Chem"; 
            date: "2015-09-16- 16"; volume: "26"; issue: "9"; pages: "1963-71"
COMMENT     Sequence Label: pcDNA3.1-kappa-HA-dL5-BKa
FEATURES             Location/Qualifiers
     source          1..4442
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     25..84
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             88..114
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             130..849
                     /codon_start=1
                     /product="fluorogen-activating protein composed of a dimer 
                     of the L5-MG single-chain antibody fragment (Szent-Gyorgyi 
                     et al., 2008)"
                     /label=FAP-alpha-2
                     /note="binds malachite green derivatives to produce red 
                     fluorescence"
                     /translation="QAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGHYANWFQQKPGQA
                     PRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSLSDVDGYLFGGGT
                     QLTVLSGGGGSGGGGSGGGGSGGGGSQAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGH
                     YANWFQQKPGQAPRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSL
                     SDVDGYLFGGGTQLTVLS"
     primer_bind     complement(4347..4366)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4401..4420
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggatccggct tggggatatc caccatggag acagacacac tcctgctatg ggtactgctg
       61 ctctgggttc caggttccac tggtgactat ccatatgatg ttccagatta tgctggggcc
      121 cagccggccc aggccgtcgt tacccaagaa cctagtgtta ccgttagccc aggtggtact
      181 gttatactta cttgtggaag tggtacgggt gccgtcacat ctggtcatta tgcaaattgg
      241 tttcaacaaa aaccaggaca agctccaaga gctttgattt ttgatactga taagaagtat
      301 tcttggaccc caggtagatt ttctggatct ttgctgggag caaaggcagc tttgacaata
      361 tcagatgctc agcctgagga cgaagccgag tattactgtt ctcttagcga cgtggatggc
      421 tacttgtttg gcggtggaac acaactgacg gttctgtccg gtggtggcgg ctctggtggc
      481 ggtggcagcg gcggtggtgg ttccggaggc ggcggttctc aggctgtggt gactcaggag
      541 ccgtcagtga ctgtgtcccc aggagggaca gtcattctca cttgtggctc cggcactgga
      601 gctgtcacca gtggtcatta tgccaactgg ttccagcaga agcctggcca agcccccagg
      661 gcacttatat ttgacaccga caagaagtat tcctggaccc ctggccgatt ctcaggctcc
      721 ctccttgggg ccaaggctgc cctgaccatc tcggatgcgc agcctgaaga tgaggctgag
      781 tattactgtt cgctctccga cgttgacggt tatctgttcg gaggaggcac ccagctgacc
      841 gtcctctcca ctagtatgga tgcgctcatc ataccggtga ccatggaggt gccgtgcgac
      901 agccggggcc aacgcatgtg gtgggctttc ttggcctcct ccatggtgac tttcttcggg
      961 ggcctcttca tcatcttgct ctggcggacg ctcaagtacc tgtggaccgt ttgctgccac
     1021 tgcgggggca agacgaagga ggcccagaag ataaacaatg gctccagcca ggcagatggt
     1081 actctcaagc cagtggacga aaaagaggag gtggtggcag ccgaggtcgg ctggatgaca
     1141 tctgtgaagg actgggcagg ggtgatgata tccgcccaga cactgactgg cagagtcctg
     1201 gttgtgttag tctttgctct cagcattggt gccctcgtaa tatacttcat agactcgtca
     1261 aacccaatag aatcctgcca gaatttctac aaagatttca cattacagat cgacatggct
     1321 ttcaacgtgt tcttcctcct ctactttggc ttgcggttta ttgcagccaa cgataagctg
     1381 tggttctggc tggaagtgaa ttcagtagta gatttcttca cagtccctcc tgtgtttgtg
     1441 tctgtgtact taaacagaag ttggcttggc ttgagatttt taagagctct cagactgata
     1501 cagttttcag agattttgca gtttctgaat atccttaaaa caagtaactc catcaagctg
     1561 gtgaatctgc tctccatatt tatcagcacg tggctgactg cagctggatt catccacttg
     1621 gtggagaatt caggggaccc atgggaaaat ttccaaaaca accaggcact tacgtactgg
     1681 gaatgtgtct acttactcat ggtcacaatg tctacagtgg gttatgggga cgtttatgca
     1741 aaaaccacac ttggacgcct cttcatggtc ttcttcatcc tcgggggact ggccatgttt
     1801 gccagctacg tccctgaaat catagagtta ataggaaacc gcaagaaata cgggggctcc
     1861 tatagcgcgg ttagtggaag aaagcacatt gtagtctgtg gacacattac tctggagagt
     1921 gtctctaact tcctgaagga ctttctgcac aaggaccggg atgatgtcaa cgtggagatt
     1981 gtctttcttc acaacatctc ccctaacctt gagcttgagg ctctgttcaa acggcatttc
     2041 actcaggtgg agttttatca gggctctgtc ctcaatccac atgatcttgc cagagtcaag
     2101 atagagtcag cagatgcatg cctgatcctt gccaataagt attgcgctga cccggatgca
     2161 gaagatgcct ccaacatcat gagagtgatc tccatcaaaa actaccaccc aaagatcagg
     2221 atcatcactc agatgctgca gtatcacaac aaggcccatc tgctcaacat ccccagctgg
     2281 aactggaaag agggtgatga cgcaatatgc cttgcagagc tcaagttggg tttcatagcc
     2341 cagagctgtc tggctcaagg cctctccaca atgcttgcca atctcttctc tatgaggtca
     2401 ttcataaaga ttgaggaaga cacatggcag aaatactact tggaaggagt ctccaatgaa
     2461 atgtacacag aatatctctc cagtgccttc gtgggtctgt ccttccctac tgtttgtgag
     2521 ctgtgttttg tgaagcttaa gctcctgatg atagccattg agtacaagtc tgccaacaga
     2581 gagagccgaa gccgaaagcg aatattaatt aaccctggga accaccttaa gatccaagaa
     2641 ggtactttag gatttttcat cgcaagtgat gccaaagaag ttaaaagggc atttttttac
     2701 tgcaaggcct gtcatgatga cgtcacagat cccaaaagaa ttaaaaaatg tggctgcagg
     2761 cggcttgaag atgagcagcc gccaaccctg tcaccaaaaa aaaaacaacg taatgggggc
     2821 atgaggaact cgcccaacac ctccccgaag ctgatgaggc atgacccctt gttaattcct
     2881 ggcaatgatc agattgacaa catggactcc aatgtgaaaa agtacgactc cactggaatg
     2941 tttcactggt gtgcacccaa ggagattgag aaagtcatct tgactcgaag tgaagctgcc
     3001 atgactgtcc tgagtggcca tgtcgtagtc tgcatctttg gggatgtcag ctcagccctg
     3061 attggcctcc ggaacctggt gatgccactc tgtgctagca actttcacta tcatgagctc
     3121 aaacacattg tgtttgtggg ctccattgag tacctcaaga gggagtggga aacactgcac
     3181 aacttcccga aagtgtccat attgcctggt acaccattaa gtcgggctga tttaagggct
     3241 gtcaacatca acctctgtga catgtgcgtt atcctgtcag ccaatcagaa taatattgat
     3301 gatacttcgc ttcaggacaa ggaatgcatc ttggcgtcac tcaacatcaa atctatgcag
     3361 tttgatgaca gcatcggggt cttgcaggct aattcccaag gattcacacc tcctggaatg
     3421 gacagatcat cacccgacaa cagcccagtg cacgggatgt tacgccagcc gtccatcaca
     3481 actggggtca acattcccat catcacggaa ctcgctaagc cgggcaagtt gcctttggta
     3541 tcagtcaatc aggaaaaaaa cagtgggacg cacattctaa tgataacgga gttggtgaat
     3601 gataccaatg ttcagttttt ggaccaagac gatgacgatg accctgacac agagctgtac
     3661 ctcacacagc cctttgcttg tgggacagca tttgccgtca gcgtcctgga ctcactcatg
     3721 agcgcgacat acttcaatga caatatcctc accctaatac ggaccctggt gacaggagga
     3781 gccacaccag agctcgaggc tctaatagct gaggagaatg cacttcgagg aggctacagc
     3841 actccgcaga cattggccaa cagggaccgt tgccgagtgg cccagttagc cctgttagat
     3901 ggtccctttg cagacttagg ggatggtggt tgttatggtg atctgttctg caaagctctg
     3961 aaaacatata atatgctttg ttttggaatt taccggctga gagatgccca cctcagcacc
     4021 cccagccagt gtacaaaaag gtacgtcatc accaatcctc cctacgagtt tgagctcgta
     4081 ccaacagacc tgatcttctg cctgatgcag tttgaccaca acgctggcca atcccgggcc
     4141 agtctgtctc attcctccca ctcctcacag tcgtccagta agaagagctc ctccgtccac
     4201 tccatcccgt ccacagcaaa tcggccgaac cggcccaagt ccagggagtc ccgcgacaaa
     4261 cagaacagaa aagaaatggt ttacagatag atcgatagaa ctatagtgag tcgtattacg
     4321 tagatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     4381 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     4441 gc
//
