LOCUS       sequence-193457-        8633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a chimeric OCT4-activator fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.07"; journalName: "Cell Rep"; date: 
            "2017-08-15- 15"; volume: "20"; issue: "7"; pages: "1585-1596"
COMMENT     Sequence Label: pSN16
FEATURES             Location/Qualifiers
     source          1..8633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(526..528,529..531,532..1242)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLFIQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(571..592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(832..851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1179..1200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1252..1308
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2395..2505
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     misc_feature    2521..3109
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2574..2594)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2992..3003)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3262..3442
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3514..3635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3551..3570)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3605..3624
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3654..3789
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3809..3828)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3810..3828)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3838..3855)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3838..3854)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3847..3869)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3996..4451
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4083..4102)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4293..4314
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4477..4581
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4582..4650,4651..5442)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4800..4819)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5613..6201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6102..6121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6355..6372
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6489..6510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6525..6530,6531..6548,6549..6555)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6563..6579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6568..6590
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6587..6603
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6587..6603
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6622..6642
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6624..6642
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6670..6896
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6897..7077
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7124..7249
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7742..7975
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8160..8204
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8471..8588
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      601 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      661 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      721 agctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcagcttcaa ggacgacggc
      841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      901 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      961 tacaacagcc acaacgtcta tatcacggcc gacaagcaga agaacggcat caaggcgaac
     1021 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gttcatccag
     1141 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1201 accgccgccg ggatcactca cggcatggac gagctgtaca agggatctgg agcaacaaac
     1261 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccggccctat ggcgggacac
     1321 ctggcttcgg atttcgcctt ctcgccccct ccaggtggtg gaggtgatgg gccagggggg
     1381 ccggagccgg gctgggttga tcctcggacc tggctaagct tccaaggccc tcctggaggg
     1441 ccaggaatcg ggccgggggt tgggccaggc tctgaggtgt gggggattcc cccatgcccc
     1501 ccgccgtatg agttctgtgg ggggatggcg tactgtgggc cccaggttgg agtggggcta
     1561 gtgccccaag gcggcttgga gacctctcag cctgagggtg aagcaggagt cggggtggag
     1621 agcaactccg atggggcctc cccggagccc tgcaccgtca cccctggtgc cgtgaagctg
     1681 gagaaggaga agctggagca aaacccggag gagtcccagg acatcaaagc tctgcagaaa
     1741 gaactcgagc aatttgccaa gctcctgaag cagaagagga tcaccctggg atatacacag
     1801 gccgatgtgg ggctcaccct gggggttcta tttgggaagg tattcagcca aacgaccatc
     1861 tgccgctttg aggctctgca gcttagcttc aagaacatgt gtaagctgcg gcccttgctg
     1921 cagaagtggg tggaggaagc tgacaacaat gaaaatcttc aggagatatg caaagcagaa
     1981 accctcgtgc aggcccgaaa gagaaagcga accagtatcg agaaccgagt gagaggcaac
     2041 ctggagaatt tgttcctgca gtgcccgaaa cccacactgc agcagatcag ccacatcgcc
     2101 cagcagcttg ggctcgagaa ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag
     2161 ggcaagcgat caagcagcga ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct
     2221 ttctcagggg gaccagtgtc ctttcctctg gccccagggc cccattttgg taccccaggc
     2281 tatgggagcc ctcacttcac tgcactgtac tcctcggtcc ctttccctga gggggaagcc
     2341 tttccccctg tctctgtcac cactctgggc tctcccatgc attcaaaccc ggccgacgcc
     2401 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
     2461 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaagtcgac
     2521 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2581 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2641 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2701 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2761 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2821 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2881 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     2941 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3001 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3061 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
     3121 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3181 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atcctgctag
     3241 ctccggatcc ggacctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     3301 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3361 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3421 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     3481 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     3541 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3601 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     3661 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3721 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3781 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     3841 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3901 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3961 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     4021 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4081 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4141 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4201 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4261 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4321 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4381 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4441 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4501 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4561 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4621 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4681 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4741 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4801 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4861 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4921 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4981 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5041 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5101 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5161 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5221 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5281 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5341 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5401 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5461 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5521 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6001 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6241 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6301 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6361 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6421 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6481 aacgcaatta atgtgagtta gctcactcat taggcacccc aggttttaca ctttatgctt
     6541 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     6601 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     6661 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     6721 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     6781 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     6841 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     6901 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6961 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7021 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     7081 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     7141 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     7201 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     7261 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     7321 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     7381 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     7441 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     7501 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     7561 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     7621 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     7681 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     7741 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     7801 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     7861 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     7921 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     7981 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     8041 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     8101 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     8161 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     8221 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     8281 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     8341 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     8401 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     8461 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     8521 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     8581 aaaattttat cgatcacgag actagcctcg agaagcttga tatcgaattc cac
//
