LOCUS       sequence-193490-       10477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses
            in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. 
            unc-54 3'UTR in sequence as well..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Lee JI, O'Halloran DM, Eastham-Anderson J, Juang BT, Kaye JA, Scott 
            Hamilton O, Lesch B, Goga A, L'Etoile ND
  TITLE     Nuclear entry of a cGMP-dependent kinase converts transient into 
            long-lasting olfactory adaptation.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):6016-21. Epub 2010 Mar
            10.
  PUBMED    20220099
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2010-03-30"; volume: "107(13)"; pages: 
            "6016-21. Epub 2010 Mar 10"
COMMENT     Sequence Label: (p)odr-3::GFP::EGL-4
FEATURES             Location/Qualifiers
     source          1..10477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    91..112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(127..132,133..150,151..157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    165..181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     170..192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     189..205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     189..205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3330..3346)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3331..3347)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3400..3428)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4176..4196
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     4276..4292
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4276..4292
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8247..8269)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8369..8388
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8470..8488)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8556..8660
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8661..8729,8730..9521)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8879..8898)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9692..10280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10181..10200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10434..10451
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
       61 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      121 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      181 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga
      241 ctctagagga tccccatctc aacatagtag atttttaaaa atagtgtttc ctttgactgt
      301 attcccattg accgttggaa cttatttcaa taagtttttc atttttcccg ccgattcttt
      361 tcataaaaat tgtgttaatc tactaacttg ttcactgatc tttgaataat tagcatgtac
      421 cccagtgttt tccgtgtctg cttcagcccc agaaggcttt tattgtaaag tattcgaaac
      481 aaattatgct ttccgagcga caaaacacat ttttttaaga atgaaaactt ggcgtcttca
      541 tcttacttta tcctactttc ttactttcag agaaacttcc aatttttttt tcacattttg
      601 tagccatgtg ggttttcgag ctccttgata gaattattca attacacgtc atttttgtgt
      661 ttcattattc gggttaaacg tctaaagaga tcagtgttct agttgtagac atgagttcag
      721 aacgtacgta ataaacacta ttcccaagtt ggatgtctcc aatattcttt tgttagactt
      781 ccagctgata attttatgtc tagaaatgag ttactcgtaa ttaaaatgtg ttgattgccc
      841 aatagattgt atgatttttg tgaagtaata ggtaattgcc acctataatt ttcaaaaccg
      901 agacatactt atttggtaat tcagtttggt aattcagcaa gttttaaaat ataattaaat
      961 taatatttct tattccaaaa tatatttcga attccaaatt tctaatttta taaaactaga
     1021 ttattttact caatcgccca cgttgtgttg actttcatat ctcaatatca tattctcaat
     1081 tccttaatct tattctcaca accaacgtcc agtgttacct gtccacttgc cttttccatc
     1141 tcaattcctt cactcattcg gtgactctca cgtctctaag gagaaaatac gaaagcaaat
     1201 atactcaagt cttttttccg ctttcttggt tgaatgactg aaatttttta gttattatct
     1261 gtattttccg gatcacccag ctgaaggaac tgatcgtagt attttgtaat ttttaaacag
     1321 aaaggtgtag gaggcgtctt ctacctaatt ctgattcata aatgttttta atgaaatccg
     1381 aacgacaatt catgttggtt tcaactacaa tcattcctct agaacaaatc cttttgtact
     1441 tatgttcttg ttttttgata ctccattttt accctctcac cacgcccatt ttaaaatata
     1501 attttcttcc tgttaactaa cataattaaa aaattaaatt atcatgtgat atgtttcaca
     1561 accttttttt caataactat tttattgaat ttttagtcat tcatgtgacg ttgtctagga
     1621 gacacctcgt ggtttttgtc tctcacgccc cttttcttcg attttttttg taggtacaaa
     1681 atacaaacag taaataaacc attgaaaaac catcgaaaaa aacagttgta tatattaagt
     1741 ttaaatatat cgagaaaaag tgttcctgaa aatttcacct gttgttgaac attcaataaa
     1801 gaaaagtgtg cgatagatat atttaaattt aagagatcga tagtgtaaat aattgttgga
     1861 tttattcccg ttttgtggct tatcagctct ttgttcggtc tgaaaaaatg ctataaaagt
     1921 tttagaaacg ctttggtatt caagaaaaaa gtgttatcaa aaaatcactc aagccttgct
     1981 tttgtcacca aatatgaaaa actcgtaaat gattttgtac gggtttatag taattaagta
     2041 gtaaatacat tttttaaaac gatttatttc caactctgaa aatttaaaaa actagtatga
     2101 atattcacgt ccttgttttg aatatgttta catggtgatc gatttttttt ggcgtaaagt
     2161 tggatgggac actttttaat tctaggaatt ttattcttga ttgttgttct ttagcaatct
     2221 acatcgagtt ttttttttaa ttttctcggt tttcacattt tggtgtatat tgaacaattc
     2281 aaactaaaaa tcttcccaaa tctcagtcca tcttcaagaa atattaatcg aaattatcag
     2341 tctttacgat ctatcaacat tgttatattt tgctctccat tctgatcaca aagaaagcca
     2401 aatgttttct ctgttcattt cctagatttc ctgagtttgt agtaggtgca cttgccttct
     2461 ccccagcttc ttcttcatct tctcgactgc aacgatacaa atcgtttggc gccgacagaa
     2521 ggatcgtggt gtcttgtgac gcacttactc taacacaatc cattctccac ccacctttta
     2581 cctccatcca tcttcttctt cttctctttt tctattgata tttgctctta ttcgagtgta
     2641 ggttatcaac gctaatcccc atcgaacaat acaggcattt tgtcgtcgtc gacgccgttt
     2701 tcttcttctt cgtcgtcgtc tttgactttg gatttatgaa ggcgacggag ccggcgatgt
     2761 aagctaagtg cagttgatat acgagcagat aagattatct cttttcttca acttttactc
     2821 catttacctc caatctttat acttcaaatg catttgtgta ttactctttt tttattgttc
     2881 tgttattccg atatttcaaa tcaattactc atagatcatt gtttttttag atgggattgg
     2941 gcagaaaatt cctttttttt ctgaaaattt gagcaaaaat tgccgaattt tcttcaaaaa
     3001 tgatgttttt ttcgcaaaaa ctcaaatttc gtgtgaacat tttcggaaat ttatcgaaaa
     3061 aactgcattt tttcgctgaa aatttgaatt tcctctcgat ttctctctca cttttccgca
     3121 tttttttccg ttttttcctc cccgctccgc tcaaaaaaat caataaataa atacttttac
     3181 agatcaaaag gtgatacctc cgacgcccgc catccaccac atccactcac aatccgaaaa
     3241 ttaatcgata aaacaataaa gcgaccagcg gaaaatcaac tggaaatgag cggatccccc
     3301 gggctgcagg aattcgatat caagcttatc gataccgtcg acctcgaggg ggcggtaccg
     3361 gtagaaaaaa tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     3421 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     3481 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
     3541 gtaagtttaa acatatatat actaactaac cctgattatt taaattttca gccaacactt
     3601 gtcactactt tctgttatgg tgttcaatgc ttctcgagat acccagatca tatgaaacgg
     3661 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     3721 aaagatgacg ggaactacaa gacacgtaag tttaaacagt tcggtactaa ctaaccatac
     3781 atatttaaat tttcaggtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     3841 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
     3901 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
     3961 gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca gaacttcaaa
     4021 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     4081 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     4141 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     4201 gctgggatta cacatggcat ggatgaacta tacaaatgcc cgggggatcg gtggagctcc
     4261 accggtggcg gccgctctag aactagtgga tccccgggat tggccaaagg acccaaaggt
     4321 atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt gagggtaccg
     4381 atgagctctg ggagccgtcc gtcgagcggc ggtggtggtg gtggcggagg agccagtggt
     4441 ggagccggtg gtggtgctcc aggtggtggt ggaggtggaa tccgtggatt cttctccaaa
     4501 cttcgtaagc cgtctgatca gccgaatggg aatcaggtac aagtcggcac acgaacgttc
     4561 gaggctcacg agcttcagaa gctgattcca cagctcgaag aggcgatatc tcggaaggat
     4621 gcacaactca gacagcaaca gacaattgtt gaaggacata ttaagaggat ttccgagttg
     4681 gagggagagg ttaccacgct tcagagagaa tgcgacaaat tgcgttccgt actggaacaa
     4741 aaagctcaat cagctgccag ccccggcgga caaccaccat caccatctcc acgtaccgat
     4801 caacttggca atgatctaca gcaaaaggcc gtgctaccgg ccgacggagt tcaacgagcc
     4861 aaaaagattg ccgtttcagc agagccgacg aattttgaga ataagccagc cacacttcaa
     4921 cactataata agactgtcgg agccaaacaa atgatacgtg acgccgtgca gaagaacgat
     4981 ttcctgaagc aattggcaaa ggagcaaata attgagctcg tcaactgtat gtacgagatg
     5041 agagcacgcg ccggacaatg ggttatacaa gaaggagaac ctggagatcg acttttcgtc
     5101 gttgccgaag gagaattgca agtttcacgt gagggagctc ttcttggaaa aatgcgcgcc
     5161 gggaccgtta tgggagagtt ggctattctc tacaactgca caagaaccgc atcagttcaa
     5221 gcattaaccg acgtacaatt atgggtgctg gatcgatccg tattccaaat gattacacag
     5281 agattgggaa tggaacgtca ctcgcagttg atgaatttct tgacaaaggt atcaatcttc
     5341 caaaatttgt cggaagatcg catctcaaag atggccgacg tcatggatca ggactattat
     5401 gatggtggac attatatcat tcgacagggt gaaaagggtg acgcattctt cgtaatcaac
     5461 tcaggacaag tgaaagtcac ccaacaaatc gaaggagaaa ccgagccccg agaaatccgt
     5521 gtgctcaatc aaggagactt ctttggagaa cgtgcactgc tcggcgagga ggtccgtaca
     5581 gccaatatta ttgcacaagc tccaggagtt gaagtactta ctttggatcg agaatcattc
     5641 ggaaagctta tcggagatct tgaaagcctt aaaaaagact atggagataa ggaacgtttg
     5701 gctcaagttg tccgagagcc gccgagccct gtgaagattg tcgacgactt ccgagaggag
     5761 tttgcacagg ttacactgaa aaatgtgaag aggctagcga cacttggagt tggaggattt
     5821 ggaagagttg aacttgtctg tgtaaatgga gataaggcaa agacgtttgc gctgaaggct
     5881 ctgaagaaga agcatattgt tgatacccgg caacaggagc acatctttgc cgagcggaat
     5941 attatgatgg aaacttcaac tgattggatt gtcaagcttt ataagacttt tagagatcaa
     6001 aagtttgtgt acatgctgct cgaggtatgc cttggcggtg aactgtggac gacactccgc
     6061 gatcgaggac actttgacga ttacaccgcc cgtttctacg tggcctgcgt gctcgagggt
     6121 ctcgaatatc tgcatcgcaa gaatattgtc taccgagatt tgaagcccga aaactgtctg
     6181 ctcgccaaca ctggatatct gaaacttgtg gatttcggat tcgcaaagaa actcgcgtct
     6241 ggaaggaaga cgtggacatt ctgtggaaca ccggaatacg tgtcacctga aattattctc
     6301 aataagggac acgatcaagc cgccgactat tgggctcttg gaatatatat ctgtgaactg
     6361 atgctcggac ggccaccgtt ccaggctagt gatcctatga agacgtatac attgatccta
     6421 aagggagtag acgcactgga gattccgaat cgtcggattg gaaagacggc gacggcgttg
     6481 gtgaagaagc tctgcaggga taacccggga gaacggctcg gcagtggaag tggtggggtg
     6541 aatgatattc ggaagcatcg gtggtttatg ggattcgatt gggaaggatt gagaagtagg
     6601 acgttgaaac ctccaattct gccgaaggtg tccaacccag cagacgtgac gaatttcgac
     6661 aattatccac cagataatga tgttccacca gacgagtttt ccggatggga tgagggattc
     6721 taggcgtccg atcttgctct tctgctgctc cgtcgttttg tgctcattct caccccacca
     6781 aaaccgccca aattttcata attttcagtc gattttcagt gttcactctg tattagtttt
     6841 ttttttctgt gtatatatat atatcacctc tcctcattat gttcggttag tgtaccgccg
     6901 ccgcccgtct cgcccgccaa ataaatttca aattttccag agattttccc cccgttttct
     6961 tgttcttttt ccaccatgtt cactgagatt ctatgtgatc ttttccggtt tctctatgat
     7021 tttttgaaac tcntaccgcn cactatttta gtattctcga attttcatat attcaccccc
     7081 tcctcctctt ttttctcagt tcctcatttt cagtctaaca ccatttttta gtttcccaac
     7141 aaaaaagtcc gagagaaaac tcggcaaact acaccaccac caggtaatat tattattttc
     7201 taatcccccc ctaatactga tatatctcta ttttcatttc tatctaaaat ccaatcattc
     7261 gaaattgaac tatacatact tttttttctt tgctctctct cttatgtctc gattctatat
     7321 ttttgattct tttctatgtg ttttttttcg tggaatgaat tattgatacg aacaaaaaaa
     7381 aaaaaaaaaa aactcgaggg ggggcccggt accatggtat tgatatctga gctccgcatc
     7441 ggccgctgtc atcagatcgc catctcgcgc ccgtgcctct gacttctaag tccaattact
     7501 cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc
     7561 aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa
     7621 attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc
     7681 ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt
     7741 atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata
     7801 ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg
     7861 cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt
     7921 tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct
     7981 attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag
     8041 cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga
     8101 agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac
     8161 attcccaata taccaaacat aactgtttct actagtcggc cgtacgggcc ctttcgtctc
     8221 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     8281 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     8341 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     8401 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct
     8461 taattaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     8521 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8581 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8641 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8701 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8761 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8821 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8881 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8941 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     9001 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     9061 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     9121 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9181 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9241 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9301 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9361 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9421 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9481 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     9541 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9601 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9661 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9721 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9781 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9841 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9901 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9961 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    10021 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    10081 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    10141 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10201 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10261 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    10321 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    10381 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    10441 cagtgagcga ggaagcggaa gagcgcccaa tacgcaa
//
