LOCUS       sequence-193511-        7181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Tyrosine Ammonia Lyase from constitutive XylA 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Jones JA, Vernacchio VR, Collins SM, Shirke AN, Xiu Y, Englaender 
            JA, Cress BF, McCutcheon CC, Linhardt RJ, Gross RA, Koffas MAG
  TITLE     Complete Biosynthesis of Anthocyanins Using E. coli Polycultures.
  JOURNAL   MBio. 2017 Jun 6;8(3). pii: e00621-17. doi: 10.1128/mBio.00621-17.
  PUBMED    28588129
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2017-06-6"; volume: "8(3). pii: e00621-17. doi"; pages: " 
            10.1128/mBio.00621-17"
COMMENT     Sequence Label: pXylA-RgTALsyn
FEATURES             Location/Qualifiers
     source          1..7181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     572..591
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(611..688)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     894..913
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3222..3240)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3236..3283
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3327..3782
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3624..3645
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(3897..4688,4689..4757))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4520..4539
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4758..4850)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4931..5519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5420..5439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5673..5690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5705..5844
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5770..5789)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5930..5952
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5946..6137)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(6709..7181,1..610))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
       61 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
      121 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
      181 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
      241 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
      301 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
      361 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
      421 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
      481 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
      541 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
      601 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
      661 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
      721 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
      781 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
      841 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
      901 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
      961 ccggcgtagc ctaggttgaa ataaacattt attgtatatg atgagataaa gttagtttat
     1021 tggataaaca aactaactca attaagatag ttgatggata aactttctag agttcactta
     1081 aatcaaaggg ggaaatgtac acatatggcg cctcgcccga cttcgcaaag ccaggcccgc
     1141 acttgcccga cgacgcaggt tacccaagtt gatatcgttg agaaaatgtt ggcggctcct
     1201 actgatagca cgctggagct ggacggttat agcctgaatc tgggtgatgt cgtgagcgct
     1261 gcgcgtaagg gtcgtcctgt ccgtgtcaaa gatagcgatg aaatccgcag caaaatcgac
     1321 aagagcgttg aattcctgcg cagccaactg agcatgtcgg tttacggtgt gacgaccggc
     1381 tttggcggct ccgcggacac gcgcacggag gacgcaatta gcctgcaaaa ggcgttgctg
     1441 gaacaccagc tgtgtggtgt gttgccgagc agcttcgaca gctttcgctt gggtcgtggt
     1501 ctggagaata gcctgccgtt ggaagtcgtt cgcggtgcaa tgaccattcg tgtgaattcg
     1561 ctgacccgtg gccatagcgc tgttcgtctg gttgttctgg aagcactgac gaactttctg
     1621 aaccacggta ttaccccgat tgttccgctg cgcggtacga tctccgcgag cggcgatctg
     1681 tctccactgt cgtacattgc agcggcgatt agcggtcacc cggatagcaa agttcacgtg
     1741 gtccatgaag gcaaagagaa gatcctgtac gcgcgcgaag cgatggcgct gtttaacctg
     1801 gagccggtgg ttttgggtcc gaaggagggc ctgggtctgg tgaatggtac ggcagtctcc
     1861 gcgagcatgg caacgctggc actgcacgac gcgcacatgt tgagcctgtt gagccaatcg
     1921 ctgaccgcga tgaccgtgga ggcgatggtc ggtcacgcgg gcagcttcca tccattcctg
     1981 cacgatgtta cgcgtccgca cccgacgcaa atcgaggtcg cgggtaacat tcgcaaactg
     2041 ctggagggct cgcgcttcgc ggtccaccac gaggaagagg ttaaggtcaa ggatgatgaa
     2101 ggcattttgc gtcaggatcg ttatccgttg cgcacgagcc cgcaatggtt gggtccgctg
     2161 gtgtccgacc tgattcacgc tcatgccgtc ttgacgatcg aagcgggtca aagcaccacc
     2221 gataacccac tgatcgatgt tgagaataag accagccatc acggtggcaa ctttcaagcg
     2281 gcagcggttg ccaacacgat ggaaaagacc cgtctgggct tggcccaaat cggtaaactg
     2341 aatttcaccc agctgacgga gatgctgaac gcgggcatga atcgtggctt gccgagctgc
     2401 ctggcggctg aagacccatc cctgagctat cattgcaaag gtctggacat tgcggcggct
     2461 gcatatacga gcgaactggg ccacctggct aacccggtca ccacccacgt ccaaccggct
     2521 gaaatggcaa accaggcggt gaatagcttg gcgttgatta gcgcacgtcg taccacggaa
     2581 tctaacgacg ttctgtccct gctgctggca acgcacctgt actgcgtgct gcaggcgatc
     2641 gacctgcgtg cgattgagtt cgagttcaag aaacagtttg gtcctgccat tgttagcctg
     2701 atcgaccaac actttggtag cgcgatgacg ggtagcaatc tgcgtgatga gctggttgaa
     2761 aaggtcaata agactctggc caagcgtttg gagcaaacca atagctacga tctggttccg
     2821 cgctggcacg acgcttttag cttcgctgca ggcactgttg tcgaggttct gtccagcacg
     2881 agcctgagct tggcggccgt gaacgcatgg aaggttgcgg cagccgagag cgcgatctcc
     2941 ttgacgcgcc aggtccgtga aacgttttgg tccgctgcaa gcacctccag cccggcgttg
     3001 tcttacttga gcccgcgcac gcagatcctg tacgcatttg tgcgtgagga actgggtgtc
     3061 aaagcccgcc gtggtgacgt cttcttgggt aaacaagaag ttaccatcgg cagcaacgtt
     3121 agcaagattt acgaagccat caagagcggc cgtatcaaca atgttctgct gaagatgctg
     3181 gcataaacta gtcgcagctt aattaaccta aactgctgcc accgctgagc aataactagc
     3241 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctagcga aaggaggagt
     3301 cgactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     3361 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3421 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3481 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3541 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3601 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3661 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3721 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     3781 ttctggcggc acgatggcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3841 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3901 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3961 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4021 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4081 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4141 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4201 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4261 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4321 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4381 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4441 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4501 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4561 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4621 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4681 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4741 aaatgttgaa tactcatact cttccttttt caatcatgat tgaagcattt atcagggtta
     4801 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggtcatga
     4861 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4921 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4981 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5041 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     5101 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5161 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5221 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5281 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5341 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5401 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5461 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5521 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5581 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5641 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5701 agcgcctgat gcggtatttt ctccttacac atctgtgcgg tatttcacac cgcaatggtg
     5761 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     5821 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     5881 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     5941 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
     6001 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
     6061 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     6121 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
     6181 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
     6241 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
     6301 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
     6361 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
     6421 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
     6481 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
     6541 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgctagtc
     6601 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga
     6661 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
     6721 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6781 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
     6841 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
     6901 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
     6961 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
     7021 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
     7081 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
     7141 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga t
//
