LOCUS       sequence-193537-        5526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase validation vector with GTSE1 enhancer inserted downstream
            of the reporter gene. The candidate's enhancer activity is reflected
            by luciferase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534."
COMMENT     Sequence Label: STARR-seq luciferase validation vector_SCP1_GTSE1
FEATURES             Location/Qualifiers
     source          1..5526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    206..297
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(238..257)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    311..359
                     /note="synthetic polyadenylation signal"
     CDS             join(464..532,533..1324)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1524..2112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2013..2032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3756..3877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3793..3812)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3847..3866
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(3921..5526,1..47))
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 gggtagaatg gcgctgggcc cttcttaatg tttttggcat cttccatggt ggctttacca
       61 acagtaccgg attgccaagc tttaggtcgc tagcccgggt ccgtaggcac gtctgctcgg
      121 ctcgagtgtt cgatcgcgac tgaggacgaa cgcgccccca cccccttata taagtcccta
      181 gggagatctc taagccagtt aggccagaga aatgttctgg cacctgcact tgcactgggg
      241 acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc gtatgttagt
      301 actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg
      361 cggcctgtcc aatacctccc gtaccttaat attacttact tatccttgag agacgtacta
      421 gtaaccctga taaatgcttc aataatattg aaaaaggacg agtatgagca tccaacattt
      481 tcgtgtcgca ctcattccct tctttgcggc attttgcttg cctgtttttg cacaccccga
      541 aacgctggtg aaagtaaaag atgctgaaga tcaactgggt gcaagagtgg gctatatcga
      601 actggatctc aatagcggca agatccttga gtctttccgc cccgaagaac gattcccgat
      661 gatgagcact ttcaaagtac tgctatgtgg cgcggtgttg tcccgtatag acgccgggca
      721 agagcagctt ggtcgccgta tacactactc acaaaacgac ttggttgagt actcgccggt
      781 cacggaaaag catcttacgg atggcatgac ggtaagagaa ttgtgtagtg ctgccattac
      841 catgagcgac aacaccgcgg ccaacttact tctgacaacg atcggaggcc ctaaggagct
      901 gactgcattt cttcataata tgggtgatca tgtgacccgg cttgaccgct gggaaccaga
      961 gttgaacgaa gccataccga acgacgagcg tgataccacg atgccagtag caatggccac
     1021 aactcttcgg aaactactca ctggcgaact tcttactcta gcatcacgac agcagctcat
     1081 agactggatg gaggcggaca aagtagcagg accacttctt cgctcggccc tccctgctgg
     1141 ctggttcatt gctgacaaat cgggggccgg tgaacgcggc tctcgcggca tcattgctgc
     1201 gctggggcct gatggtaagc cctcacgaat cgtagtgatc tacacgacgg ggagtcaggc
     1261 cactatggac gaacgaaata ggcagatcgc tgagatcggt gcctcactga tcaagcactg
     1321 gtaaccactg cagtggttta gcatttgcgg ccgctgtcag accaagttta ctcatatata
     1381 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1441 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1501 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1561 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1621 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1681 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1741 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1801 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1861 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1921 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1981 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2041 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2101 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2161 tttgctcaca tggagccagt gtctgggggc gtggtggcgg gactggacct ctggggcctg
     2221 tgttgggccc tagctcggta actggtgacc cctttgccct ctgagcctgc ttttcatcct
     2281 gttgtgaata tcagatgaga tcatacacat ggaaatgctt gtcaactaaa acagcacaaa
     2341 cacagtgtaa tgaattatta ttatcccagt aaagtcatgg ttcgggaaaa tgagggaatg
     2401 tgggctctgt tgcgcgtgcc tgggaagcta aatccctgcg gtgttgttcc tgtgggcaag
     2461 tgtctagcgc atgcaggtgc cgtctgacaa gtcgacttcg ctctgtgaag gtcgtgctcg
     2521 gccctgatgg gatatacagc cagggtgggg cggcgcagtg aagatggctc ttctctttct
     2581 cccccgtgtc catccatcag accacagcct ttgcctgggc aggggtcccc gtgtaggggt
     2641 gaatgcctct catgtggctg cctgcagcgg ggtggccctt tgtgtacttg tctgagccag
     2701 tgccacctac actcaagttg ggacagtgga gatactcgga gaattgccca ttcggtgacg
     2761 atcccccaac agtaggtctc cccctgatca ctttacctga ccgcacactt cctttcttgg
     2821 acttaggtga ctccccggac agctcaacac caaagctttc gcgggcacag cggccgcagt
     2881 cgtgcacgtc agttggcagg tgagtgacgt tggtccgctt cctctcttta ttgctgtaga
     2941 atgactcacg catctgctta cctcaaccac ttggtaaccc tgcagcctgg cctgcgcatc
     3001 tggcttccac gccagtctct tgggtgcagg ccgagctcct gatgtgtacc agcccctttt
     3061 agatctactg cagacttaag acatctgaga cttccgcgat gcagtacggc agctcccagc
     3121 cctggactgg tgtggttgcc atgccaggct gcacagcaga agtccctcca gcgctcttag
     3181 gactgcccgt gcccagccca ccccaggggt tctgatttgg ttcatctagg ctgaggccca
     3241 gcagctggat ttcctaaagc tcctgggtga ctgtgacttg taccgcagtt gaagagcact
     3301 ggcgtggcac tttgattctg acttggcttt ttttttcttt ttgagatgga gtcttgctct
     3361 gtcgcccagg ctggagtgca gtggcacgat ctcggctcac tgcaaactcc acctcccggg
     3421 ttcacgccat tctcctgcct cagcctccct agtagctggg actacaggca cccgccacca
     3481 cacccggcta attatttttg tatttttttt agtagagatg gggtttcacc gtgttagcca
     3541 ggatggtctc gatctcctgg cctcgtgatc cgcccgcctc tgcctcccaa agtgctggga
     3601 ttataggcgt gagccaccgt gtccggcctg acttggcttt gaatcccagc tcccttatcg
     3661 attttaccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     3721 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     3781 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3841 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgctcg aagcggccgg
     3901 ccgccccgac tctagaatta ttacacggcg atcttgccgc ccttcttggc cttaatgaga
     3961 atctcgcgga tcttgcgggc gtccaacttg ccggtcagtc ctttaggcac ctcgtccacg
     4021 aacacaacac caccgcgcag cttcttggcg gttgtaacct ggctggccac atagtccacg
     4081 atctccttct cggtcatggt tttaccgtgt tccagcacga cgactgcggc gggcagctcg
     4141 ccggcatcgt cgtcgggcag gccggcgacc ccggcgtcga agatgttggg gtgttgcagc
     4201 aggatgctct ccagttcggc tggggctacc tggtagccct tgtatttgat caggctcttc
     4261 agccggtcca cgatgaagaa gtgctcgtcc tcgtcccagt aggcgatgtc gccgctgtgc
     4321 agccagccgt ccttgtcgat gagagcgttt gtagcctcgg ggttgttaac gtagccgctc
     4381 atgatcatgg ggccacggac gcacagctcg ccgcgctggt tcacacccag tgtcttaccg
     4441 gtgtccaagt ccaccacctt agcctcgaag aagggcacca ccttgcctac tgcgccaggc
     4501 ttgtcgtccc cttcgggggt gatcagaatg gcgctggttg tttctgtcag gccgtagccc
     4561 tggcggatgc ctggtaggtg gaagcgtttg gccacggcct cacctacctc cttgctgagc
     4621 ggcgccccgc cgctggcgat ctcgtgcaag ttgcttaggt cgtacttgtc gatgagagtg
     4681 ctcttagcga agaagctaaa tagtgtgggc accagcaggg cagattgaat cttatagtct
     4741 tgcaagctgc gcaagaatag ctcctcctcg aagcggtaca tgagcacgac ccgaaagccg
     4801 cagatcaagt agcccagcgt ggtgaacatg ccgaagccgt ggtgaaatgg caccacgctg
     4861 aggatagcgg tgtcggggat gatctggttg ccgaagatgg ggtcgcgggc atgactgaat
     4921 cggacacaag cggtgcggtg cggtagggct acgcccttgg gcaatccggt actgccacta
     4981 ctgttcatga tcagggcgat ggttttgtcc cggtcgaagc tctcgggcac gaagtcgtac
     5041 tcgttgaagc cgggtggcaa atgggaagtc acgaaggtgt acatgctttg gaagccctgg
     5101 tagtcggtct tgctatccat gatgatgatc ttttgtatga tcggtagctt cttttgcacg
     5161 ttgaggatct tttgcagccc tttcttgctc acgaatacga cggtgggctg gctgatgccc
     5221 atgctgttca gcagctcgcg ctcgttgtag atgtcgttag ctggggccac agccacaccg
     5281 atgaacaggg cacccaacac gggcatgaag aactgcaagc tattctcgct gcacaccacg
     5341 atccgatggt ttgtattcag cccatagcgc ttcatagctt ctgccagccg aacgctcatc
     5401 tcgaagtact cggcgtaggt aatgtccacc tcgatatgtg cgtcggtaaa ggcgatggtg
     5461 ccgggcacca gggcgtagcg cttcatggct ttgtgcagct gctcgccggc ggtcccgtct
     5521 tcgagt
//
