LOCUS       sequence-193544-        3621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BKa (KCNMA1, mSlo alpha subunit) in mammalian cells. 
            (tagless, leaderless).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Pratt CP, He J, Wang Y, Barth AL, Bruchez MP
  TITLE     Fluorogenic Green-Inside Red-Outside (GIRO) Labeling Approach 
            Reveals Adenylyl Cyclase-Dependent Control of BKalpha Surface 
            Expression.
  JOURNAL   Bioconjug Chem. 2015 Sep 16;26(9):1963-71. doi: 
            10.1021/acs.bioconjchem.5b00409. Epub 2015 Sep 2.
  PUBMED    26301573
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.bioconjchem.5b00409"; journalName: "Bioconjug Chem"; 
            date: "2015-09-16- 16"; volume: "26"; issue: "9"; pages: "1963-71"
COMMENT     Sequence Label: pcDNA3.1-BKa
FEATURES             Location/Qualifiers
     source          1..3621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    3473..3607
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3497..3516)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3551..3570
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctagtatgga tgcgctcatc ataccggtga ccatggaggt gccgtgcgac agccggggcc
       61 aacgcatgtg gtgggctttc ttggcctcct ccatggtgac tttcttcggg ggcctcttca
      121 tcatcttgct ctggcggacg ctcaagtacc tgtggaccgt ttgctgccac tgcgggggca
      181 agacgaagga ggcccagaag ataaacaatg gctccagcca ggcagatggt actctcaagc
      241 cagtggacga aaaagaggag gtggtggcag ccgaggtcgg ctggatgaca tctgtgaagg
      301 actgggcagg ggtgatgata tccgcccaga cactgactgg cagagtcctg gttgtgttag
      361 tctttgctct cagcattggt gccctcgtaa tatacttcat agactcgtca aacccaatag
      421 aatcctgcca gaatttctac aaagatttca cattacagat cgacatggct ttcaacgtgt
      481 tcttcctcct ctactttggc ttgcggttta ttgcagccaa cgataagctg tggttctggc
      541 tggaagtgaa ttcagtagta gatttcttca cagtccctcc tgtgtttgtg tctgtgtact
      601 taaacagaag ttggcttggc ttgagatttt taagagctct cagactgata cagttttcag
      661 agattttgca gtttctgaat atccttaaaa caagtaactc catcaagctg gtgaatctgc
      721 tctccatatt tatcagcacg tggctgactg cagctggatt catccacttg gtggagaatt
      781 caggggaccc atgggaaaat ttccaaaaca accaggcact tacgtactgg gaatgtgtct
      841 acttactcat ggtcacaatg tctacagtgg gttatgggga cgtttatgca aaaaccacac
      901 ttggacgcct cttcatggtc ttcttcatcc tcgggggact ggccatgttt gccagctacg
      961 tccctgaaat catagagtta ataggaaacc gcaagaaata cgggggctcc tatagcgcgg
     1021 ttagtggaag aaagcacatt gtagtctgtg gacacattac tctggagagt gtctctaact
     1081 tcctgaagga ctttctgcac aaggaccggg atgatgtcaa cgtggagatt gtctttcttc
     1141 acaacatctc ccctaacctt gagcttgagg ctctgttcaa acggcatttc actcaggtgg
     1201 agttttatca gggctctgtc ctcaatccac atgatcttgc cagagtcaag atagagtcag
     1261 cagatgcatg cctgatcctt gccaataagt attgcgctga cccggatgca gaagatgcct
     1321 ccaacatcat gagagtgatc tccatcaaaa actaccaccc aaagatcagg atcatcactc
     1381 agatgctgca gtatcacaac aaggcccatc tgctcaacat ccccagctgg aactggaaag
     1441 agggtgatga cgcaatatgc cttgcagagc tcaagttggg tttcatagcc cagagctgtc
     1501 tggctcaagg cctctccaca atgcttgcca atctcttctc tatgaggtca ttcataaaga
     1561 ttgaggaaga cacatggcag aaatactact tggaaggagt ctccaatgaa atgtacacag
     1621 aatatctctc cagtgccttc gtgggtctgt ccttccctac tgtttgtgag ctgtgttttg
     1681 tgaagcttaa gctcctgatg atagccattg agtacaagtc tgccaacaga gagagccgaa
     1741 gccgaaagcg aatattaatt aaccctggga accaccttaa gatccaagaa ggtactttag
     1801 gatttttcat cgcaagtgat gccaaagaag ttaaaagggc atttttttac tgcaaggcct
     1861 gtcatgatga cgtcacagat cccaaaagaa ttaaaaaatg tggctgcagg cggcttgaag
     1921 atgagcagcc gccaaccctg tcaccaaaaa aaaaacaacg taatgggggc atgaggaact
     1981 cgcccaacac ctccccgaag ctgatgaggc atgacccctt gttaattcct ggcaatgatc
     2041 agattgacaa catggactcc aatgtgaaaa agtacgactc cactggaatg tttcactggt
     2101 gtgcacccaa ggagattgag aaagtcatct tgactcgaag tgaagctgcc atgactgtcc
     2161 tgagtggcca tgtcgtagtc tgcatctttg gggatgtcag ctcagccctg attggcctcc
     2221 ggaacctggt gatgccactt cgtgctagca actttcacta tcatgagctc aaacacattg
     2281 tgtttgtggg ctccattgag tacctcaaga gggagtggga aacactgcac aacttcccga
     2341 aagtgtccat attgcctggt acaccattaa gtcgggctga tttaagggct gtcaacatca
     2401 acctctgtga catgtgcgtt atcctgtcag ccaatcagaa taatattgat gatacttcgc
     2461 ttcaggacaa ggaatgcatc ttggcgtcac tcaacatcaa atctatgcag tttgatgaca
     2521 gcatcggggt cttgcaggct aattcccaag gattcacacc tcctggaatg gacagatcat
     2581 cacccgacaa cagcccagtg cacgggatgt tacgccagcc gtccatcaca actggggtca
     2641 acattcccat catcacggaa ctcgctaagc cgggcaagtt gcctttggta tcagtcaatc
     2701 aggaaaaaaa cagtgggacg cacattctaa tgataacgga gttggtgaat gataccaatg
     2761 ttcagttttt ggaccaagac gatgacgatg accctgacac agagctgtac ctcacacagc
     2821 cctttgcttg tgggacagca tttgccgtca gcgtcctgga ctcactcatg agcgcgacat
     2881 acttcaatga caatatcctc accctaatac ggaccctggt gacaggagga gccacaccag
     2941 agctcgaggc tctaatagct gaggagaatg cacttcgagg aggctacagc actccgcaga
     3001 cattggccaa cagggaccgt tgccgagtgg cccagttagc cctgttagat ggtccctttg
     3061 cagacttagg ggatggtggt tgttatggtg atctgttctg caaagctctg aaaacatata
     3121 atatgctttg ttttggaatt taccggctga gagatgccca cctcagcacc cccagccagt
     3181 gtacaaaaag gtacgtcatc accaatcctc cctacgagtt tgagctcgta ccaacagacc
     3241 tgatcttctg cctgatgcag tttgaccaca acgctggcca atcccgggcc agtctgtctc
     3301 attcctccca ctcctcacag tcgtccagta agaagagctc ctccgtccac tccatcccgt
     3361 ccacagcaaa tcggccgaac cggcccaagt ccagggagtc ccgcgacaaa cagaacagaa
     3421 aagaaatggt ttacagatag atcgatagaa ctatagtgag tcgtattacg tagatccaga
     3481 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     3541 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     3601 acaagttaac aacaacaatt c
//
