LOCUS       sequence-193566-        7029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  It contains ~2.7 Kb of the miR-183-96-182 cluster promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Segura MF, Jubierre L, Li S, Soriano A, Koetz L, Gaziel-Sovran A, 
            Masanas M, Kleffman K, Dankert JF, Walsh MJ, Hernando E
  TITLE     Kruppel-like factor 4 (KLF4) regulates the miR-183~96~182 cluster 
            under physiologic and pathologic conditions.
  JOURNAL   Oncotarget. 2017 Apr 18;8(16):26298-26311. doi: 
            10.18632/oncotarget.15459.
  PUBMED    28412746
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget"; journalName: "Oncotarget"; date: "2017-04-18-
            18"; volume: "8"; issue: "16"; pages: "26298-26311"
COMMENT     Sequence Label: pGL4-Cluster Promoter Delta 3
FEATURES             Location/Qualifiers
     source          1..7029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..143
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     92..111
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2966..2997
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3024..3033
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3030..4682
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4726..4847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4737..4756)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4791..4810
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5095..5112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5266..5854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5346..5365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6054..6845,6846..6914))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    join(7019..7029,1..38)
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
       61 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
      121 tgcaggtgcc agaacatttc tctggcctaa ctggccggta cctgagctcg ctagcctcga
      181 ggataacggt cttcaagtgc aagggttagg tttggtggga gcataggcta agcaccaccc
      241 tcattttaca ggggcttcta gggtgccaga tctgcggggg tcgattggga agaactagaa
      301 gctaaaaagg agaagggcgg attaacgttt tattttaatc tggttttgag ctgccgttgc
      361 cgtctggggc ggccgggcgg ggcggagctg ggactgccaa gggcagccaa agcagcaggg
      421 agggaggggg cgctccggtg ctcaggatgc tggggaagaa ggaagaggcc accccaacgc
      481 tgacagcttg gaggacctag cccctgcccc ccggtccctt ctgaatgctc cgggtggtgt
      541 cagaccaaag gacggttgag aaggatctaa tccgcgtccg cgccccagcc ccggctggcc
      601 ccgccccctc ccgagggagc cactgtcgca gtggggggag ggggagatgg gaacgggtct
      661 gggggcggct cccctccccc tccctctctt gggacagaca gcctttgttc ccggcggcgg
      721 cggcagcggt ggccagacac ctgtgaggcc gcagaactgg ccgagggtgg gcgtgtgagg
      781 acatggaggc cccttgtccc cattccagcc ctgcgcccct gggtccgggt gcgccccctg
      841 agacaggtca gccttgagca cctttctgcg gggagactgc agagatcgtc cagtggccct
      901 acggtgcgcc ccagctccca acgcacttcc cctcccccct cccagggtcg gatttccaag
      961 cacgccacgc cacacacacc tcggctagct ccgggctcag agggcgctgg ggtggcaggg
     1021 cgtccgggcg cacctcggct agcggcaccc tagcccgagg gcaggaggcg gggatagaag
     1081 gcgggcgacg ggaccccttg gggccagacc cggccgggtt gggcaagcag gcggggctcc
     1141 cggcgtctcc gcagtcccgg acgggcgtgc cctgcgccgc gcaccaaccg gagccacatt
     1201 caaggtgaat catccccggc ccgccccctg cccctccccg cctcgcggca cctgagccaa
     1261 tggcgctgcg cgaggggcgg gcggggcccg gggcgggggg cggtaaaacc ctcctggagt
     1321 cattctctgt gcactccgtc gagcccccgc gcagccttgc cggagccgca gggcctccgt
     1381 ccagccgcgc ggtccgggca gcccgcgccc cctgttcttc cggtcctcgg gcccaggtac
     1441 ccctctggac ctcccccgcc tgcagcgcgg aggcggctcc tcttcaggtg gccgggccgg
     1501 ggccttccct ggcagacctg tcacccgaca cccaggaagc gcgcggccgg gtcccggctc
     1561 ccctttgttc ccccgctggg ctcggcgctc cggcgccgcc ccctctcgcg gggcagcgga
     1621 ggtttgggcc cgggccgggc gcgcttcaag ctccgtccat gcggctcttt ccaaaccggg
     1681 gccgtttttg cctggcttct ctggtgtgga ctccgcgcgg agctgagagg cggctgtgcg
     1741 cccagcctgg agccggcgca ggtgagtgtc tggggccggg agcggggggg cgggccgggc
     1801 ggagccagag cggggctcag tagaggaggc ggaggcgggc gcccgggacc ggccgcacta
     1861 gaacccgggt gcagggcaga agtcccggcg cagctggctt tcggggatga cgcggcagtg
     1921 cctactcgct cttccgcgcc ttcaggcgga ggcgcccacg gggcgcctgg gtcccgggtc
     1981 cgccagtggc gtctgcccag cgcccagcgc tgttcccggg cgggggcggg ggcgggagcg
     2041 gctgcggtcc gagtgcggcc ggtgcttgcg aggtgcctac ccgcgaggag tttggctggg
     2101 gtcgggctgc gtttcgccgc cctggtgggc tcaggcggaa gcgccaggct gggagggggc
     2161 tgcggagagc gccggggccc cggagctggc ccctccagcg tcttgtcagg gctgcgcggc
     2221 tggttcgagg ctgcttccgt ggcccggagc cgccccgccc agtctctggg ggaaagcggc
     2281 tctcagacct cccgtgtgcc tccacttcgc ggccactcat cccgggaacc ccggcgcacg
     2341 cgaatgagac accgttcccc gccctcaact gccccattcc tgttaatagc gctagtggca
     2401 cccaggttcc cagcccttcc ccaagatcgg cggctggacg gcttgcctct ggcgcctcac
     2461 cggtcagcgc atcccaggac tatgggcagt gagggtgtca cccggggtca ggggtcgccc
     2521 gattccacgg tgtcctccag gctgagccat tcgtttgctc ttccaacccg ggctcccgga
     2581 caccgtgtac agtaataccc gccgggggca ggggcactga ggccggctct gagcgattag
     2641 ggccgagggc gggcggtgcc gtaatcgacc tccccacccc cacaggcaag gtgcggcggc
     2701 gcctcccagg tttgcgcccg tcttcgcaaa gctcctgagg gcgaaaatcc tgcgagccag
     2761 gtggcggatt ctcgggccgg gtcactcagc tctacgggtg cgcatcaccc tctacctgtc
     2821 acccagtgag cccgaccgat gggcaccgag tcggcggatc tctgatccga ggccgagcct
     2881 tcggagaaat ctgtcctagt ggcctcatcg taagggcgaa ttctgcagat atcaagatct
     2941 ggcctcggcg gccaagctta gacactagag ggtatataat ggaagctcga cttccagctt
     3001 ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt aagaagggcc
     3061 cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga
     3121 agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca
     3181 ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg
     3241 ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc
     3301 ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg
     3361 agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga
     3421 aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca
     3481 tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc
     3541 atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa
     3601 ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac
     3661 cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga
     3721 tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca
     3781 ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg
     3841 agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac
     3901 tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg
     3961 agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct
     4021 tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga
     4081 tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg
     4141 ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt
     4201 gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc
     4261 tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc
     4321 acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc
     4381 cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg
     4441 gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta
     4501 aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga
     4561 agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg
     4621 acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt
     4681 aataattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg
     4741 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     4801 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     4861 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     4921 acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt gagagccttc
     4981 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     5041 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     5101 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5161 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5221 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5281 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5341 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5401 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5461 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5521 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5581 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5641 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5701 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5761 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5821 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5881 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5941 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6001 atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag
     6061 tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc
     6121 tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca
     6181 gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca
     6241 gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg
     6301 agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg
     6361 gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct
     6421 ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc
     6481 tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta
     6541 atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc
     6601 ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc
     6661 ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc
     6721 gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg
     6781 atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg
     6841 gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa
     6901 tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag
     6961 tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa
     7021 taaaatatc
//
