LOCUS       sequence-193569-       13388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  baculovirus-based delivery system that enables the efficient 
            incorporation of unnatural amino acids into proteins in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13388)
  AUTHORS   Chatterjee A, Xiao H, Bollong M, Ai HW, Schultz PG
  TITLE     Efficient viral delivery system for unnatural amino acid mutagenesis
            in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. doi: 
            10.1073/pnas.1309584110. Epub 2013 Jul 1.
  PUBMED    23818609
REFERENCE   2  (bases 1 to 13388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1309584110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-16- 16"; volume: "110"; issue: "29"; pages: "11803-8"
COMMENT     Sequence Label: pAcBac2.tR4-OMeYRS/GFP*
FEATURES             Location/Qualifiers
     source          1..13388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  83..248
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        complement(354..448)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    354..372
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    382..400
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(538..786)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1123..1142
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1141..1196
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1242..1261
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     misc_feature    1327..1915
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1433..1444
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1842..1862
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1941..1958)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1974..2003)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(2013..2723,2724..2726,2727..2729))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT*GKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2055..2076)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2404..2423
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2663..2684
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2726..2735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2772..2791)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          2799..3815
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(2853..2875)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(3816..4091)
                     /label=chicken beta-actin promoter
     enhancer        4093..4396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    4474..4608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4498..4517)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4552..4571
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(4735..6222,6223..6270))
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     promoter        complement(6329..6420)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(6387..6404)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     enhancer        6515..6894
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6895..7098
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7049..7069
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7143..7162
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7143..7161
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7181..7190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8535..8552)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8541..8765
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8802..9050
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        9140..9234
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    9188..9206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9216..9234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(9737..9742,9743..9759,9760..9765)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             9954..10487
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  10554..10778
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(10912..10929)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11083..11671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11163..11182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11842..12633,12634..12702))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12465..12484
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12703..12807)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(12833..13287)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12970..12991)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13181..13200
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aaagtagccg aagatgacgg tttgtcacat ggagttggca ggatgtttga ttaaaaacat
       61 aacaggaaga aaaatgcccc gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg
      121 aaatggagtt tttaaggatt atttagggaa gagtgacaaa atagatggga actgggtgta
      181 gcgtcgtaag ctaatacgaa aattaaaaat gacaaaatag tttggaacta gatttcactt
      241 atctggttcg gatctcctag caaaaaatgg agggggacgg attcgaaccg ccgaacccaa
      301 agggagcgga tttagagtcc gccgcgttta gccacttcgc tacccctccg gtgtctctat
      361 cactgatagg gaacttataa gtctctatca ctgataggga tttcacgttt atggtgattt
      421 cccagaacac atagcgacat gcaaatatta aaaaatggtg ggggaaggat tcgaaccttc
      481 gaagtctgtg acggcagatt tagagtctgc tccctttggc cgctcgggaa ccccaccggt
      541 gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt
      601 aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta
      661 ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattct
      721 aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag
      781 gccctcttcc tgcccgacct agggcaggtc gagggatctc cataagagaa gagggacagc
      841 tatgactggg agtagtcagg agaggaggaa aaatctggct agtaaaacat gtaaggaaaa
      901 ttttagggat gttaaagaaa aaaataacac aaaacaaaat ataaaaaaaa tctaacctca
      961 agtcaaggct tttctatgga ataaggaatg gacagcaggg ggctgtttca tatactgatg
     1021 acctctttat agccaacctt tgttcatggc agccagcata tgggcatatg ttgccaaact
     1081 ctaaaccaaa tactcattct gatgttttaa atgatttgcc ctcccatatg tccttccgag
     1141 tgagagacac aaaaaattcc aacacactat tgcaatgaaa ataaatttcc tttattagcc
     1201 agaagtcaga tgctcaaggg gcttcatgat gtccccataa tttttggcag agggaaaaag
     1261 atctcagtgg tatttgtgag ccagggcatt ggtcgatcga ccactgtgct ggcggccggc
     1321 caggccgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac
     1381 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
     1441 ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca
     1501 gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg cccaacagcc
     1561 gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga
     1621 gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg
     1681 gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact
     1741 cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg
     1801 aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa
     1861 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattgtcga
     1921 cttaacgcgt tgaattctca atggtgatgg tgatgatgac cggtatgcat attcagatcc
     1981 tcttctgaga tgagtttttg ttcgaagggc cccttgtaca gctcgtccat gccgagagtg
     2041 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     2101 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     2161 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
     2221 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
     2281 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     2341 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     2401 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
     2461 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
     2521 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
     2581 gtgcagatga acttcagggt cagcttgccc taggtggcat cgccctcgcc ctcgccggac
     2641 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
     2701 gtgaacagct cctcgccctt gctcaccatg gtggcctcct tggccaattc tttgccaaaa
     2761 tgatgagaca gcacaataac cagcacgttg cccaggagct gtaggaaaaa gaagaaggca
     2821 tgaacatggt tagcagaggc tctagagccg ccggtcacac gccagaagcc gaaccccgcc
     2881 ctgccccgtc ccccccgaag gcagccgtcc ccccgcggac agccccgagg ctggagaggg
     2941 agaaggggac ggcggcgcgg cgacgcacga aggccctccc cgcccatttc cttcctgccg
     3001 gcgccgcacc gcttcgcccc gcgcccgcta gagggggtgc ggcggcgcct cccagatttc
     3061 ggctccgcac agatttggga caaaggaagt ccctgcgccc tctcgcacga ttaccataaa
     3121 aggcaatggc tgcggctcgc cgcgcctcga cagccgccgg cgctccgggg gccgccgcgc
     3181 ccctcccccg agccctcccc ggcccgaggc ggccccgccc cgcccggcac ccccacctgc
     3241 cgccaccccc cgcccggcac ggcgagcccc gcgccacgcc ccgtacggag ccccgcaccc
     3301 gaagccgggc cgtgctcagc aactcgggga ggggggtgca gggggggttg cagcccgacc
     3361 gacgcgccca caccccctgc tcaccccccc acgcacacac cccgcacgca gcctttgttc
     3421 ccctcgcagc cccccccgca ccgcggggca ccgcccccgg ccgcgctccc ctcgcgcaca
     3481 ctgcggagcg cacaaagccc cgcgccgcgc ccgcagcgct cacagccgcc gggcagcgcg
     3541 gagccgcacg cggcgctccc cacgcacaca cacacgcacg caccccccga gccgctcccc
     3601 ccgcacaaag ggccctcccg gagcccctca aggctttcac gcagccacag aaaagaaaca
     3661 agccgtcatt aaaccaagcg ctaattacag cccggaggag aagggccgtc ccgcccgctc
     3721 acctgtggga gtaacgcggt cagtcagagc cggggcgggc ggcgcgaggc ggcggcggag
     3781 cggggcacgg ggcgaaggca gcgcgcagcg actcccgccc gccgcgcgct tcgcttttta
     3841 tagggccgcc gccgccgccg cctcgccata aaaggaaact ttcggagcgc gccgctctga
     3901 ttggctgccg ccgcacctct ccgcctcgcc ccgccccgcc cctcgccccg ccccgccccg
     3961 cctggcgcgc gccccccccc cccccgcccc catcgctgca caaaataatt aaaaaataaa
     4021 taaatacaaa attgggggtg gggagggggg ggagatgggg agagtgaagc agaacgtggg
     4081 gctcacctcg accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     4141 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     4201 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     4261 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
     4321 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
     4381 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
     4441 ttactattaa taactaggct caagcagtga tcagatccag acatgataag atacattgat
     4501 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     4561 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     4621 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     4681 aacctctaca aatgtggtat ggctgattat gatcctctag tacttctcga cttatcactt
     4741 tccaagtcgg ttcatctcta tgtctgtata aatctgtctt ttcttggtgt gctttaattt
     4801 aatgcaaaga tggataccaa ctcggagaac caagaatagt ccaatgatta accctatgat
     4861 aaagaaaaaa gaggcaatag agcttttcca actactgaac caaccttcta caagctcgat
     4921 tggatttttg gatagcccag tatcaccaaa aaataaactc tcatcatcag gaagttgcga
     4981 agcagcgtct tgaatgtgag gatgttcgaa cacctgagcc tttgagctaa gatgaagatc
     5041 ggagtccaac ataccatgtc caatcatgta taaaggaaac ttatatcctg aactggtcct
     5101 cagaactcca ttgggtccaa tttccacgtc ttcatatggt gcccagtcat cccacagttc
     5161 cctttctgtg gtagttccac tgatcattcc gaccattctt gagaggattg gagcagcaat
     5221 atcgactctg atgtatctgg tctcaaagta ttttagggta ccattgatta tggtgaaagc
     5281 aggaccggtt cctgggtttt taggagcaag atagctgaga tccactggag agattggaag
     5341 acccgctctg attttgctcc aggtttcttg gcagagggaa taatccaaga tcctctcaac
     5401 gtcctgaatt agacttacat ccactgaggt ctgagatgga gcagagatac ttgacccttc
     5461 tgggcattca gggaatctgg ctgcagcaaa gagatcctta tcagccatct cgaaccagac
     5521 acctgatggg agtctgactc cccaatgctt gcagtattgc attttgcagg ccttgcctcc
     5581 agtttcataa gcaaagtagt tacttctgaa ccctgtgccc tcctttccca gggatgatag
     5641 ctctccgtcc tctgagaaga aggtgatgtc catggaaatg aggttagaat cacatagccc
     5701 tttgacctta tagtcagaat gccaggttgt agagttatgg acagtggggc atatgtaatt
     5761 gctgcatttt ccgttgatga actgtgaatc aacccattct cctgtgtatt catcaaccag
     5821 cacatggtga ggagtcacct ggacaatcac tgcttcggca tccgtcacag ttgcatatcc
     5881 acaactttga ggagggaagc ctggattcag ccaagttcct tgtttcgttt gttcaatgct
     5941 ttccttgcat tgttctacag atggagtgaa ggatcggatg gaatgtgtta tatacttcgg
     6001 tccataccag cggaaatcac aagtagtgac ccatttggaa gcatgacaca tccaaccgtc
     6061 tgcttgaata gccttgtgac tcttgggcat tttgacttgt aaggctgtgc ctattaagtc
     6121 attatgccaa tttaaatctg agcttgacgg gcaataatgg taattagaag gaacattttt
     6181 ccagtttcct ttttggttgt gtggaaaaac tatggtgaac ttgcaattca ccccaatgaa
     6241 taaaaaggct aagtacaaaa ggcacttcat ggtggcggtt ttttaggagt tcgcgcccga
     6301 tggtgggacg gtatgaataa tccggaatat ttataggttt ttttattaca aaactgttac
     6361 gaaaacagta aaatacttat ttatttgcga gatggttatc attttaatta tctccatgat
     6421 ctattaatat tccggagtat acggaccgcg gccgcaaata cctgcaggat ccgttttgcg
     6481 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
     6541 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     6601 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     6661 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     6721 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     6781 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     6841 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     6901 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     6961 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     7021 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     7081 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
     7141 attaatacga ctcactatag ggagacccaa gctggctagc gccaccatgg caagcagtaa
     7201 cttgattaaa caattgcaag agcgggggct ggtagcccag gtgacggacg aggaagcgtt
     7261 agcagagcga ctggcgcaag gcccgatcgc actcgtgtgt ggcttcgatc ctaccgctga
     7321 cagcttgcat ttggggcatc ttgttccatt gttatgcctg aaacgcttcc agcaggcggg
     7381 ccacaagccg gttgcgctgg taggcggcgc gacgggtctg attggcgacc cgagcttcaa
     7441 agctgccgag cgtaagctga acaccgaaga aactgttcag gagtgggtgg acaaaatccg
     7501 taagcaggtt gccccgttcc tcgatttcga ctgtggagaa aactctgcta tcgcggccaa
     7561 taattatgac tggttcggca atatgaatgt gctgaccttc ctgcgcgata ttggcaaaca
     7621 cttctccgtt aaccagatga tcaacaaaga agcggttaag cagcgtctca accgtgaaga
     7681 tcaggggatt tcgttcactg agttttccta caacctgctg cagggttata gtatggcctg
     7741 tttgaacaaa cagtacggtg tggtgctgca aattggtggt tctgaccagt ggggtaacat
     7801 cacttctggt atcgacctga cccgtcgtct gcatcagaat caggtgtttg gcctgaccgt
     7861 tccgctgatc actaaagcag atggcaccaa atttggtaaa actgaaggcg gcgcagtctg
     7921 gttggatccg aagaaaacca gcccgtacaa attctaccag ttctggatca acactgcgcg
     7981 tgccgacgtt taccgcttcc tgaagttctt cacctttatg agcattgaag agatcaacgc
     8041 cctggaagaa gaagataaaa acagcggtaa agcaccgcgc gcccagtatg tactggcgga
     8101 gcaggtgact cgtctggttc acggtgaaga aggtttacag gcggcaaaac gtattaccga
     8161 atgcctgttc agcggttctt tgagtgcgct gagtgaagcg gacttcgaac agctggcgca
     8221 ggacggcgta ccgatggttg agatggaaaa gggcgcagac ctgatgcagg cactggtcga
     8281 ttctgaactg caaccttccc gtggtcaggc acgtaaaact atcgcctcca atgccatcac
     8341 cattaacggt gaaaaacagt ccgatcctga atacttcttt aaagaagaag atcgtctgtt
     8401 tggtcgtttt accttactgc gtcgcggtaa aaagaattac tgtctgattt gctggaaagg
     8461 gcccgtttaa gaattcaacg cgttaagtcg actttaactc gagtctagag ggcccgttta
     8521 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     8581 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     8641 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     8701 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     8761 tatggcttct gaggcggaaa gaaccagatc tcgggcagga agagggccta tttcccatga
     8821 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
     8881 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
     8941 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
     9001 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc ggtggggttc
     9061 ccgagcggcc aaagggagca gactctaaat ctgccgtcac agacttcgaa ggttcgaatc
     9121 cttcccccac cattttttaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa
     9181 cgtgaaatcc ctatcagtga tagagactta taagttccct atcagtgata gagacaccgg
     9241 aggggtagcg aagtggctaa acgcggcgga ctctaaatcc gctccctttg ggttcggcgg
     9301 ttcgaatccg tccccctcca ttttttcagc tggggctcta gggggtatcc ccacgcgccc
     9361 tgtagcggcg cattaagcgc aagccctgcc atagccacta cgggtacgta gccaaccact
     9421 agaactatag ctagagtcct gggcgaacaa acgatgctcg ccttccagaa aaccgaggat
     9481 gcgaaccact tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc gaggtctacc
     9541 gatctcctga agcaagggca gatccgtgca cagcaccttg ccgtagaaga acagcaaggc
     9601 cgccaatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag
     9661 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat
     9721 gaaggcacga acccagttga cataagcctg ttcggttcgt aaactgtaat gcaagtagcg
     9781 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     9841 ggcggttttc atggcttgtt atgactgttt ttttgtacag tctatgcctc gggcatccaa
     9901 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     9961 gcagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta ggtggctcaa
    10021 gtatgggcat cattcgcaca tgtaggctcg gccctgacca agtcaaatcc atgcgggctg
    10081 ctcttgatct tttcggtcgt gagttcggag acgtagccac ctactcccaa catcagccgg
    10141 actccgatta cctcgggaac ttgctccgta gtaagacatt catcgcgctt gctgccttcg
    10201 accaagaagc ggttgttggc gctctcgcgg cttacgttct gcccaagttt gagcagccgc
    10261 gtagtgagat ctatatctat gatctcgcag tctccggcga gcaccggagg cagggcattg
    10321 ccaccgcgct catcaatctc ctcaagcatg aggccaacgc gcttggtgct tatgtgatct
    10381 acgtgcaagc agattacggt gacgatcccg cagtggctct ctatacaaag ttgggcatac
    10441 gggaagaagt gatgcacttt gatatcgacc caagtaccgc cacctaacaa ttcgttcaag
    10501 ccgagatcgg cttcccggcc gcggagttgt tcggtaaatt gtcacaacgc cgcgaatata
    10561 gtctttacca tgcccttggc cacgcccctc tttaatacga cgggcaattt gcacttcaga
    10621 aaatgaagag tttgctttag ccataacaaa agtccagtat gctttttcac agcataactg
    10681 gactgatttc agtttacaac tattctgtct agtttaagac tttattgtca tagtttagat
    10741 ctattttgtt cagtttaaga ctttattgtc cgcccacacc cgcttacgca gggcatccat
    10801 ttattactca accgtaaccg attttgccag gttacgcggc tggtctatgc ggtgtgaaat
    10861 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
    10921 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10981 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    11041 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    11101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    11161 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    11221 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    11281 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    11341 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    11401 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    11461 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    11521 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    11581 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    11641 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    11701 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    11761 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    11821 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    11881 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    11941 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    12001 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    12061 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    12121 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    12181 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    12241 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    12301 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    12361 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    12421 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    12481 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    12541 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    12601 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    12661 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    12721 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    12781 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
    12841 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
    12901 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
    12961 agtgttgttc cagtttggaa caagagtcca ctattaagaa cgtggactcc aacgtcaaag
    13021 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
    13081 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
    13141 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
    13201 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
    13261 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcaa ataagcgttg
    13321 atattcagtc aattacaaac attaataacg aagagatgac agaaaaattt tcattctgtg
    13381 acagagaa
//
