LOCUS       sequence-193579-        6103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HA tag fused to the N-terminus of RILP for the expression in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Wozniak AL, Long A, Jones-Jamtgaard KN, Weinman SA
  TITLE     Hepatitis C virus promotes virion secretion through cleavage of the 
            Rab7 adaptor protein RILP.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12484-12489. Epub 2016 
            Oct 17.
  PUBMED    27791088
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2016-11-1"; volume: "113(44)"; pages: 
            "12484-12489. Epub 2016 Oct 17"
COMMENT     Sequence Label: pTRE2-Bla(HA–RILP)
FEATURES             Location/Qualifiers
     source          1..6103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..335
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      186..321
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     248..267
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        383..430
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             449..847
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    1005..1086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1042..1061)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    join(1101..1119,1120..1142,1143..1161,1162..1184,1185..1203,
                     1204..1226,1227..1245,1246..1268,1269..1287,1288..1310,
                     1311..1329,1330..1352,1353..1371)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        1404..1442
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1439..1463
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1628..1654
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          2885..3457
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3589..3608)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3654..3709
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3708..3727)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4107..4124)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4278..4866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4358..4377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5037..5828,5829..5897))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5660..5679
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5898..6002)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6070..6088
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
       61 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
      121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
      181 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
      241 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
      301 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
      361 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
      421 ataatacgac aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac
      481 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
      541 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
      601 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
      661 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
      721 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
      781 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
      841 gggctaagca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
      901 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
      961 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     1021 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1081 cactgcctcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     1141 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     1201 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     1261 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     1321 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     1381 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     1441 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     1501 gaagacaccg ggaccgatcc agcctccgcg gccccgaatt cgagctcggt acccggggat
     1561 cctctagtca gctgacgcgt gctagcgcgg ccgcatcgat aagcttgtcg acgaattcat
     1621 gactagttac ccatacgatg ttccagatta cgctactagt gagcccagga gggcggcgcc
     1681 cggggtgcct ggctgggggt ctcgggaggc cgcggggtcg gcatcggccg cggagcttgt
     1741 gtaccatcta gccggggccc tgggcactga gctgcaggat ctggcgcgcc gtttcgggcc
     1801 ggaggcggcg gccgggctgg tgccgctagt ggtgcgggcg ctggagctct tggaacaggc
     1861 tgccgtgggg cccgccccgg actcgctgca ggtgtcggcg cagccggcgg agcaggagct
     1921 gcggcggctg cgggaggaga acgagcgcct ccgcagggag ctgcgcgcgg ggccacagga
     1981 ggagcgcgcg ctgctgcggc agctcaagga ggtcacggac cgacagcggg acgaactccg
     2041 ggcgcacaac cgcgacctgc ggcagcgcgg ccaggagacc gaggcgttgc aggagcagct
     2101 gcagcgcctc ctgctggtga acgctgagct gcggcacaag ctggcggcca tgcagaccca
     2161 gctgcgcgcc gcgcaggacc gcgagaggga gcgccagcag cctggcgaag ccgcgactcc
     2221 gcaggctaaa gagcgagcgc gggggcaggc cgggcggccc gggcaccagc acggacagga
     2281 gcccgaatgg gcgaccgccg gcgcaggcgc cccagggaac cctgaggacc cggcggaggc
     2341 cgcgcagcag ctcgggcgcc cctcggaggc agggcagtgc cgcttcagtc gggaggagtt
     2401 tgagcagatc cttcaggagc ggaatgaact caaagccaaa gtgttcctgc tcaaggagga
     2461 actggcctac ttccagcggg agctgctcac agaccaccgg gtccccggcc ttctgctcga
     2521 ggccatgaag gtggctgtcc ggaagcagcg gaagaagatc aaggccaaga tgttagggac
     2581 accagaggaa gcagagagca gtgaggatga ggctggccca tggatcctgc tctccgatga
     2641 caagggagac catcccccac ccccggagtc caaaatacag agtttctttg gcctatggta
     2701 tcggggtaaa gctgaatcct ctgaggatga gaccagcagc cctgcaccca gcaagctagg
     2761 gggagaagag gaggcccaac cacagtctcc agctcctgat ccgccctgtt ctgccctcca
     2821 cgaacacctt tgtctggggg cctcagccgc cccagaggcc tgatctagag ctgagaactt
     2881 cagggtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt
     2941 atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca
     3001 ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc
     3061 tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta
     3121 acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact
     3181 tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat
     3241 tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa
     3301 tattcttatt ggtagaaaca actacaccct ggtcatcatc ctgcctttct ctttatggtt
     3361 acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc
     3421 tgctaaccat gttcatgcct tcttctcttt cctacagctc ctgggcaacg tgctggttgt
     3481 tgtgctgtct catcattttg gcaaagaatt cactcctcag gtgcaggctg cctatcagaa
     3541 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
     3601 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     3661 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     3721 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     3781 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     3841 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     3901 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     3961 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     4021 tctcttatgg agatccctcg actgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4081 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4141 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4201 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4261 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4681 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4861 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4921 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4981 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5041 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5101 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5161 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5221 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5281 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5341 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5401 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5461 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5521 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5581 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5641 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5701 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5761 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5821 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5881 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5941 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6001 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     6061 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aca
//
