LOCUS       sequence-19358-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Velkov T, Chuang S, Wielens J, Sakellaris H, Charman WN, Porter CJ, 
            Scanlon MJ
  TITLE     The interaction of lipophilic drugs with intestinal fatty 
            acid-binding protein.
  JOURNAL   J Biol Chem. 2005 May 6. 280(18):17769-76.
  PUBMED    15722357
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 May 6. 280(18):17769-76."
COMMENT     Sequence Label: pTrc99-Rat FABP2
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(477..499)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    488..504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(512..518,519..535,536..541))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gccaagcttg catgcctgca ggtcgactct agaggatcct gcattgcgcc aggctccaag
       61 aagctggcct attccttctt aaagatccgc ttggcctcca ctccttcata tgtgtaggtc
      121 tggattagtt cgttaccaga aatctctcgg acagcaatca gctcctttcc attgtctaca
      181 cgtttgaatt ttccaacaag tttatttccc tccatggtcc aggtcccagt gagttctgtt
      241 ccatctgcta gactataggc aaagtcgacg ccgagttcaa acacaacatc aatgtttcgg
      301 aagttgcttg attctttgac tgtgaattta tttccttcct gtgtgatcgt cagtttcagg
      361 ttgtcatgag ctccaagctt cctcttcacc acgttaatgc ccattttctc catgaacttt
      421 tcatagttct cattccggtc tactttccaa gtgccatcaa atgccatggt acctttcctg
      481 tgtgaaattg ttatccgctc acaattccac acattatacg agccggatga ttaattgtca
      541 acagctcatt tcagaatatt tgccagaacc gttatgatgt cggcgcaaaa aacattatcc
      601 agaacgggag tgcgccttga gcgacacgaa ttatgcagtg atttacgacc tgcacagcca
      661 taccacagct tccgatggct gcctgacgcc agaagcattg gtgcaccgtg cagtcgatga
      721 taagctgtca aaccagatca attcgcgcta acttacatta attgcgttgc gctcactgcc
      781 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      841 gagaggcggt ttgcgtattg g
//
