LOCUS       sequence-193581-        9614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dominant negative glutamate receptor subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Robert A, Hyde R, Hughes TE, Howe JR
  TITLE     The expression of dominant-negative subunits selectively suppresses 
            neuronal AMPA and kainate receptors.
  JOURNAL   Neuroscience. 2002;115(4):1199-210.
  PUBMED    12453491
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience."; date: "2002"; volume: "115(4)"; pages: "1199-210"
COMMENT     Sequence Label: pRH8
FEATURES             Location/Qualifiers
     source          1..9614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     708..724
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     709..725
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5367..5384)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5373..5597
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(5729..6317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5809..5828)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6646..6693
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     6718..6737
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6925..7713)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7036..7055)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7640..7659
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7748..8105)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7762..7897
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        7914..8105
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8062..8082
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(8165..8956,8957..9025))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8788..8807
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9026..9130)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9157..9612
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9244..9263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9454..9475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagctgg ctagcgttta aacttaagct tggtaccggg ccccccctcg aggtcgacgg
      721 tatcgataag cttgatatcg aattcgggct cgcaagggct tcgcaggctg gacattgtgc
      781 ttgctggatt tttcccggat gctcccggac taacatggat gtcccaccat cccttgcagt
      841 ggaagcttgc tccttggcgc agtgagagtg aagaacatgc agcgactgct aatgggtttg
      901 ggaagcggag actccttcct ctttctgtga ccatgccgtg attgtgtctg cggccactac
      961 tccacgcatc ttccttctcg tccaagcccg gagcctaacg ctagatcggg gaagtgggtg
     1021 ccgcgcgcgc aggcacggaa acatcatgaa gattatttcc ccagttttaa gtaatctagt
     1081 cttcagtcgc tccattaaag tcctgctctg cttattgtgg atcggatatt cgcaaggaac
     1141 cacacatgtg ttaagattcg gtggtatatt tgaatatgtg gaatctggcc ccatgggagc
     1201 agaagaactt gcattcagat ttgctgtgaa taccatcaac agaaacagga ctttgctgcc
     1261 caacaccact ttaacttatg atactcagaa gatcaatctc tatgacagtt ttgaagcatc
     1321 taagaaagct tgtgatcagc tgtctcttgg ggtggctgct atcttcggtc cttcacacag
     1381 ttcatcagcc aatgctgtgc agtccatctg caatgctctg ggggttcccc acatacagac
     1441 ccgctggaag caccaggtgt cagacaacaa ggattccttc tacgtcagtc tctacccaga
     1501 cttctcttcc ctgagccgcg ccatcttgga tttggtgcag ttttttaagt ggaaaactgt
     1561 cacagttgtg tatgacgaca gcactggtct cattcgcttg caagagctca tcaaagctcc
     1621 atcgaggtac aatcttcgac ttaaaattcg tcagctgcca gctgatacca aagatgcaaa
     1681 acctttgctg aaggagatga aaagaggcaa ggagttccac gtgatcttcg actgcagcca
     1741 tgagatggca gcaggcattt taaaacaggc attagctatg ggaatgatga cagaatacta
     1801 tcactatata tttacaactc tggacctctt tgctcttgac gtggagccct acagatacag
     1861 tggcgtaaat atgacagggt tcaggatact aaatacagag aatacccaag tctcctccat
     1921 catcgaaaag tggtctatgg aacggttaca ggcgcctcca aaacctgact caggtttgct
     1981 ggatggattt atgacgactg atgctgctct gatgtatgat gcagtgcacg ttgtgtctgt
     2041 ggctgtccaa cagtttcccc agatgacagt cagctccttg caatgcaatc gacacaaacc
     2101 ctggcgcttt gggacccgct tcatgagtct aattaaagag gctcactggg aaggtctcac
     2161 aggcagaata acatttaaca aaaccaatgg attacggaca gattttgatt tggatgtgat
     2221 cagtctcaag gaagaaggtc tggagaagat tggaacttgg gatccagcca gtggcctgaa
     2281 tatgacagaa agtcagaaag gaaagccagc aaatatcaca gactcattgt ctaatcgttc
     2341 tttgattgtt accaccattt tggaagaacc gtatgttctg tttaagaagt ctgacaaacc
     2401 actctatggg aatgatcgat ttgaaggcta ctgtattgat ctcctacgag agttatctac
     2461 aatccttggc tttacatatg agattaggct tgtggaggat gggaaatatg gagcccagga
     2521 tgatgtgaac ggacaatgga atggaatggt tcgtgaacta atcgatcata aagctgacct
     2581 tgcagttgct ccactggcta taacctatgt tcgtgagaag gtcatcgact tttcaaagcc
     2641 gtttatgaca cttggaataa gtattttgta ccgcaagccc aatggtacaa acccaggcgt
     2701 cttctccttc ctgaatcctc tctcccctga tatctggatg tatgttctgc tggcttgctt
     2761 gggtgtcagt tgtgtgctct ttgtcatagc caggtttagt ccctatgagt ggtataaccc
     2821 acacccttgc aaccctgact cagacgtggt ggaaaacaat tttaccttgc taaatagttt
     2881 ctggtttgga gttggagctc tcaggcggca aggttctgag ctcatgccca aagcactctc
     2941 caccaggata gtgggaggca tttggtggtt tttcacactt atcatcattt cttcgtatac
     3001 cgctaaccta gccgcctttc tgactgtgga acgcatggag tcgcccattg actctgctga
     3061 cgatttagct aagcaaacca agatagagta tggagcagtg gaggacggcg caaccatgac
     3121 gttttttaag aaatcaaaaa tttcaacgta tgataaaatg tgggcgttta tgagcagcag
     3181 gagacagtct gtgcttgtca aaagcaatga ggaagggatc caacgagtcc tcacctcgga
     3241 ttatgctttc ttaatggagt caacaaccat cgagtttgtt acacagcgga actgtaacct
     3301 cacgcagatt ggcggcctta tagactccaa aggctatggc gttggcactc ctatgggctc
     3361 tccatatcga gacaaaatca ccatagcaat tcttcagctg caggaggaag gcaagctgca
     3421 catgatgaag gagaaatggt ggcggggcaa tggctgccca gaggaggaga gcaaagaggc
     3481 cagtgctctg ggggtgcaga atattggtgg tatcttcatt gtcctggcag ccggcttggt
     3541 gctctcagtt tttgtggcag tgggagagtt tttatacaaa tccaaaaaaa acgctcaatt
     3601 ggaaaagagg tccttctgta gcgctatggt ggaagagctg agaatgtccc tgaagtgcca
     3661 gcgtcggctc aaacataagc cacaggcccc agttattgtg aaaacagaag aagttatcaa
     3721 catgcacaca tttaacgaca gaaggttgcc aggtaaagaa accatggcat gaagctggga
     3781 ggccaatcac ccaagcacaa actgtcgtct tttttttttt tttttccaaa caatttagcg
     3841 agaatgtttc ctgtggaaat atgcaacctg tgcaaaataa aatgagttac ctcatgccgc
     3901 tgtgtctatg aactagagac tctgtgatct aagcagtttc agtgatcaga cttgatttac
     3961 aagcaccgtg gatcaaccaa gttacacggg gttacactgt ttatcatagg ttcctccctt
     4021 cctttgagtg aatgttacat gcaaatgttg tggctggttt caaatgcagt ccagggagaa
     4081 actgctggtt ccttctgaag ctcagctgtc gtcaggagat ggaatgccgg tgcccaaaag
     4141 ggtaaccaat aaaaatgcca taaaaatttt aaaaaaatgc gtgagatcgg caaaaattat
     4201 agtgttacaa gaaacagtac agtcccatgg tcaccaacac aatagaggtg ataatgttac
     4261 tagcccccaa tactcagtaa aatcgtcatc tgaatagata atatgtgttc atagaatgtg
     4321 aaaaaaaatg taatgcgaga cacaccagta tcaatagaag tggaactgaa ggcagaacat
     4381 catcagttac ttttcttttt caatagtctg tgtcatggat tgtgatatag atggcaatta
     4441 tcaagccaat aatttttttt ctgaaaatac ctatggcaaa tattttaata ggcaacttgc
     4501 tcccacaaat ccctactcta acctccccca gaaatataaa aggaaccatt ggtttagaga
     4561 ttggtatgta agagatgatg ttttgcaagc cttgtcgtgc attgtaaaag ggctcagtgt
     4621 tactggttac agggaagact gaagctttca ccctgacatt ctgaaatgtc aaccgaaact
     4681 ctccttcctc ctgtaaagga ccttgatggg gcagattcca ttgatcaaag aatggggact
     4741 tgtcacctat acaatggtac gtgacagaac tttgaggtgg actgcattta ataatagtca
     4801 caatgttaaa agaacaaaat tcttgagcag tttttttttt ttgttttgtt ttgttttcaa
     4861 aaaatgttca ggtttatttg tggaaatgca agatttctat aaaatagttt ttgtatggaa
     4921 atttttgtaa tactttttat caacaaaata agaacacatg tttctgtcag gggtgtgagg
     4981 tcaagcatga acggtagtgc gtgtgcacca ccaacgtttg gtgaaactat ttttatcaag
     5041 aaaaaggaat catagaagag aaatattttc aagttagata ctataaaagc taggtgcact
     5101 accaccacgg cttgtcgcgc cacacccctg agtccacaag gtggataaca tattgtaatg
     5161 aacagttgtg tgtaaaatgg caaaagacac agacctcttg acaacattgt gaaaacagtt
     5221 gagtgcacac agtttgctgt ttgaatccaa tgcacaaaaa ttttacaaaa atccattaaa
     5281 attatgtccg ttttaaaacc ctgcagccca tccagcacag tggcggccgc tcgagtctag
     5341 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5401 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5461 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5521 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5581 tgcggtgggc tctatggctt ctgaggcgga aagaaccagt ggcggtaata cggttatcca
     5641 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5701 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5761 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5821 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5881 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5941 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6001 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6061 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6121 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     6181 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6241 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6301 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6361 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6421 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg
     6481 ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg
     6541 gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg
     6601 gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa
     6661 caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg
     6721 tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat
     6781 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
     6841 catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg
     6901 tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
     6961 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     7021 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     7081 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     7141 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag
     7201 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tgatcatcct gatcgacaag
     7261 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     7321 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     7381 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     7441 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     7501 ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc
     7561 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     7621 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     7681 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     7741 atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     7801 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     7861 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     7921 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     7981 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     8041 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc
     8101 ctcaggactc ttcctttttc aataaatcaa tctaaagtat atatgagtaa acttggtctg
     8161 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8221 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8281 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8341 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8401 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8461 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8521 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8581 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8641 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8701 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8761 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8821 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8881 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8941 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9001 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9061 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9121 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     9181 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     9241 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     9301 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     9361 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     9421 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     9481 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     9541 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     9601 cgcttacaat ttac
//
