LOCUS       sequence-193605-       10967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of human SPDEF truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Lo YH, Noah TK, Chen MS, Zou W, Borras E, Vilar E, Shroyer NF
  TITLE     SPDEF Induces Quiescence of Colorectal Cancer Cells by Changing the 
            Transcriptional Targets of beta-catenin.
  JOURNAL   Gastroenterology. 2017 Jul;153(1):205-218.e8. doi: 
            10.1053/j.gastro.2017.03.048. Epub 2017 Apr 5.
  PUBMED    28390865
REFERENCE   2  (bases 1 to 10967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2017.03.048"; journalName: "Gastroenterology"; 
            date: "2017-07"; volume: "153"; issue: "1"; pages: "205-218.e8"
COMMENT     Sequence Label: pINDUCER20-Flag-NLS-hSPDEF delta1
FEATURES             Location/Qualifiers
     source          1..10967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    51..69
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    93..111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    135..153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    177..195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    219..237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        270..308
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     305..329
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    438..462
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             468..533
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             555..575
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1324..1348)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1401..1427
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1513..2724
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2561..2580
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(2783..3400,3401..3406,3407..3442,3443..3445,3446..3481,
                     3482..3484,3485..3520,3521..3529)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3687..4149
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3743..3760)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3970..3989
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4165..4959
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4165..4959
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4219..4238)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4829..4848
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5136..5724
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5189..5209)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5607..5618)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5799..6032
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6151..6169)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6237..6341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6342..6410,6411..7202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6560..6579)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7373..7961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7862..7881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8115..8132
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8372..8393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8408..8413,8414..8431,8432..8438)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8446..8462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8451..8473
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8470..8486
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8470..8486
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8536..8555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8747..9380
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9427..9552
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10049..10282
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10467..10511
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10804..10921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
       61 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      121 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
      181 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      241 gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag gcctatataa
      301 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      361 tccatagaag acaccgggac cgatccagcc tccgcggccc cgaactagtc cagtgtggtg
      421 gaattctgca gatatcaaca agtttgtaca aaaaagcagg cttcatggac tacaaagacc
      481 atgacggtga ttataaagat catgacatcg attacaagga tgacgatgac aagcttgcgg
      541 ccgcgaattc cgcccccaag aaaaagagaa aggtgatggg cagcgccagc ccgggtctga
      601 gcagcgtatc ccccagccac ctcctgctgc cccccgacac ggtgtcgcgg acaggcttgg
      661 agaaggcggc agcgggggca gtgggtctcg agagacggga ctggagtccc agtccacccg
      721 ccacgcccga gcagggcctg tccgccttct acctctccta ctttgacatg ctgtaccctg
      781 aggacagcag ctgggcagcc aaggcccctg gggccagcag tcgggaggag ccacctgagg
      841 agcctgagca gtgcccggtc attgacagcc aagccccagc gggcagcctg gacttggtgc
      901 ccggcgggct gaccttggag gagcactcgc tggagcaggt gcagtccatg gtggtgggcg
      961 aagtgctcaa ggacatcgag acggcctgca agctgctcaa catcaccgca gatcccatgg
     1021 actggagccc cagcaatgtg cagaagtggc tcctgtggac agagcaccaa taccggctgc
     1081 cccccatggg caaggccttc caggagctgg cgggtaagga gctgtgcgcc atgtcggagg
     1141 agcagttccg ccagcgctcg cccctgggtg gggatgtgct gcacgcccac ctggacatct
     1201 ggaagtcagc ggcctggatg aaagagcgga cttcacctgg ggcgattcac tactgtgcct
     1261 cgaccagtga ggagagctgg accgacagcg aggtggactc atcatgctcc gggcagccct
     1321 gagacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc
     1381 tagagggccc gcggttcgaa tacccatacg acgtcccaga ctacgcttag taatgattaa
     1441 ttaaactaga aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagtaacc
     1501 cacccaagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
     1561 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
     1621 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
     1681 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
     1741 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
     1801 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
     1861 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg
     1921 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
     1981 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
     2041 ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     2101 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     2161 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     2221 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     2281 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     2341 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     2401 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     2461 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     2521 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     2581 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     2641 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     2701 ccgtttttgg cttttttgtt agacgaagct tggtaccgag ctcggatctc caccccgtac
     2761 cggtcctgca gtcgaattca ccatgtctag actggacaag agcaaagtca taaacggagc
     2821 tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     2881 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     2941 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga
     3001 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     3061 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     3121 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     3181 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     3241 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc
     3301 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     3361 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     3421 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     3481 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga
     3541 tccgcggccg cactagagga attccgcccc tctccccccc ccccctcccc cccccctaac
     3601 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3661 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3721 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3781 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3841 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     3901 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     3961 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     4021 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     4081 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     4141 cgatgataag cttaccggtc caccatgatt gaacaagatg gattgcacgc aggttctccg
     4201 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4261 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4321 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     4381 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4441 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4501 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4561 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4621 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4681 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4741 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4801 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4861 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4921 cgcatcgcct tctatcgcct tcttgacgag ttcttctgat gtacaagtaa agcggccgcg
     4981 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     5041 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg tttagtccct
     5101 cccaattcga tatcaagctt atcgcgatag atcctaatca acctctggat tacaaaattt
     5161 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5221 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5281 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5341 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5401 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5461 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5521 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     5581 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5641 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5701 tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc
     5761 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5821 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5881 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5941 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6001 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     6061 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga
     6121 aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6181 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6241 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6301 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6361 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6421 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6481 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6541 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     6601 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6661 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6721 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6781 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6841 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6901 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6961 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7021 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7081 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7141 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7201 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7261 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7321 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7381 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7441 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7501 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7561 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7621 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7681 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7741 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7801 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7861 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7921 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7981 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8041 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8101 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8161 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt
     8221 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8281 aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg
     8341 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8401 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8461 aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt
     8521 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     8581 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     8641 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     8701 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     8761 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     8821 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     8881 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     8941 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     9001 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     9061 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     9121 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     9181 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9241 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9301 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9361 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     9421 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9481 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9541 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     9601 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     9661 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     9721 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     9781 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     9841 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
     9901 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     9961 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    10021 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    10081 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    10141 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    10201 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    10261 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    10321 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    10381 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    10441 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    10501 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    10561 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    10621 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    10681 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    10741 ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag tattcatcca
    10801 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
    10861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
    10921 tcgggtttat tacagggaca gcagagatcc agtttggact aggatcc
//
