LOCUS       sequence-193608-       11233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human SPDEF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Lo YH, Noah TK, Chen MS, Zou W, Borras E, Vilar E, Shroyer NF
  TITLE     SPDEF Induces Quiescence of Colorectal Cancer Cells by Changing the 
            Transcriptional Targets of beta-catenin.
  JOURNAL   Gastroenterology. 2017 Jul;153(1):205-218.e8. doi: 
            10.1053/j.gastro.2017.03.048. Epub 2017 Apr 5.
  PUBMED    28390865
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2017.03.048"; journalName: "Gastroenterology"; 
            date: "2017-07"; volume: "153"; issue: "1"; pages: "205-218.e8"
COMMENT     Sequence Label: pINDUCER20-Flag-hSPDEF WT
FEATURES             Location/Qualifiers
     source          1..11233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2731..2796
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3824..3848)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3901..3927
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4013..5224
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5061..5080
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5283..5900,5901..5906,5907..5942,5943..5945,5946..5981,
                     5982..5984,5985..6020,6021..6029)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6174..6636
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6230..6247)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6457..6476
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6652..7446
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6652..7446
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6706..6725)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7316..7335
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7623..8211
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7676..7696)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8094..8105)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8286..8519
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8638..8656)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8724..8828
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8829..8897,8898..9689)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9047..9066)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9860..10448
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10349..10368
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10602..10619
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10859..10880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10895..10900,10901..10918,10919..10925)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10933..10949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10938..10960
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10957..10973
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10957..10973
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11023..11042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggcttcatg gactacaaag accatgacgg tgattataaa
     2761 gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa ttccatgggc
     2821 agcgccagcc cgggtctgag cagcgtatcc cccagccacc tcctgctgcc ccccgacacg
     2881 gtgtcgcgga caggcttgga gaaggcggca gcgggggcag tgggtctcga gagacgggac
     2941 tggagtccca gtccacccgc cacgcccgag cagggcctgt ccgccttcta cctctcctac
     3001 tttgacatgc tgtaccctga ggacagcagc tgggcagcca aggcccctgg ggccagcagt
     3061 cgggaggagc cacctgagga gcctgagcag tgcccggtca ttgacagcca agccccagcg
     3121 ggcagcctgg acttggtgcc cggcgggctg accttggagg agcactcgct ggagcaggtg
     3181 cagtccatgg tggtgggcga agtgctcaag gacatcgaga cggcctgcaa gctgctcaac
     3241 atcaccgcag atcccatgga ctggagcccc agcaatgtgc agaagtggct cctgtggaca
     3301 gagcaccaat accggctgcc ccccatgggc aaggccttcc aggagctggc gggtaaggag
     3361 ctgtgcgcca tgtcggagga gcagttccgc cagcgctcgc ccctgggtgg ggatgtgctg
     3421 cacgcccacc tggacatctg gaagtcagcg gcctggatga aagagcggac ttcacctggg
     3481 gcgattcact actgtgcctc gaccagtgag gagagctgga ccgacagcga ggtggactca
     3541 tcatgctccg ggcagcccat ccacctgtgg cagttcctca aggagttgct actcaagccc
     3601 cacagctatg gccgcttcat taggtggctc aacaaggaga agggcatctt caaaattgag
     3661 gactcagccc aggtggcccg gctgtggggc atccgcaaga accgtcccgc catgaactac
     3721 gacaagctga gccgctccat ccgccagtat tacaagaagg gcatcatccg gaagccagac
     3781 atctcccagc gcctcgtcta ccagttcgtg caccccatct gagacccagc tttcttgtac
     3841 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
     3901 tacccatacg acgtcccaga ctacgcttag taatgattaa ttaaactaga aattctaccg
     3961 ggtaggggag gcgcttttcc caaggcagtc tggagtaacc cacccaagat ctggcctccg
     4021 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     4081 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     4141 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     4201 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     4261 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     4321 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     4381 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     4441 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     4501 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     4561 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     4621 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     4681 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     4741 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     4801 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     4861 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     4921 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     4981 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     5041 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     5101 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     5161 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     5221 agacgaagct tggtaccgag ctcggatctc caccccgtac cggtcctgca gtcgaattca
     5281 ccatgtctag actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     5341 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     5401 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     5461 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     5521 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     5581 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     5641 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     5701 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     5761 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     5821 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     5881 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     5941 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     6001 attttgacct tgacatgctc cccgggtaac taagtaagga tccgcggccg cactagagga
     6061 attccgcccc tctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     6121 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     6181 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     6241 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     6301 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     6361 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     6421 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     6481 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     6541 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     6601 ggggacgtgg ttttcctttg aaaaacacga tgataagctt accggtccac catgattgaa
     6661 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     6721 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     6781 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     6841 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     6901 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6961 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     7021 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     7081 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     7141 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     7201 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     7261 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     7321 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     7381 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     7441 ttctgatgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     7501 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     7561 gaatgcaatt gttgttgttt agtccctccc aattcgatat caagcttatc gcgatagatc
     7621 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7681 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7741 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7801 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7861 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7921 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7981 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     8041 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     8101 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     8161 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     8221 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8281 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8341 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8401 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8461 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8521 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8581 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     8641 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8701 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8761 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8821 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8881 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8941 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9001 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9061 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9121 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9181 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9241 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9301 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9361 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9421 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9481 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9541 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9601 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9661 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9721 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9781 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9841 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9901 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9961 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10021 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10081 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10141 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10201 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10261 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10321 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10381 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10441 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10501 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10561 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10621 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10681 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    10741 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    10801 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    10861 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10921 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
    10981 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11041 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    11101 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    11161 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    11221 caaacttagt agt
//
