LOCUS       sequence-193609-       10505 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of human SPDEF truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Lo YH, Noah TK, Chen MS, Zou W, Borras E, Vilar E, Shroyer NF
  TITLE     SPDEF Induces Quiescence of Colorectal Cancer Cells by Changing the 
            Transcriptional Targets of beta-catenin.
  JOURNAL   Gastroenterology. 2017 Jul;153(1):205-218.e8. doi: 
            10.1053/j.gastro.2017.03.048. Epub 2017 Apr 5.
  PUBMED    28390865
REFERENCE   2  (bases 1 to 10505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1053/j.gastro.2017.03.048"; journalName: "Gastroenterology"; 
            date: "2017-07"; volume: "153"; issue: "1"; pages: "205-218.e8"
COMMENT     Sequence Label: pINDUCER20-Flag-NLS-hSPDEF delta3
FEATURES             Location/Qualifiers
     source          1..10505
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    43..61
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    127..145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    169..187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    211..229
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        262..300
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     297..321
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    430..454
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             460..525
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             547..567
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(848..872)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             925..951
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1037..2248
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2085..2104
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(2307..2924,2925..2930,2931..2966,2967..2969,2970..3005,
                     3006..3008,3009..3044,3045..3053)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3106..3679
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3273..3290)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3500..3519
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3695..4489
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3695..4489
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3749..3768)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4359..4378
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4666..5254
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4719..4739)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5137..5148)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5329..5562
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5681..5699)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5767..5871
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5872..5940,5941..6732)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6090..6109)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6903..7491
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7392..7411
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7645..7662
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7902..7923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7938..7943,7944..7961,7962..7968)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7976..7992
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7981..8003
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8000..8016
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8000..8016
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8066..8085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8277..8910
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8957..9082
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9579..9812
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9997..10041
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10334..10451
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
      301 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      361 agacaccggg accgatccag cctccgcggc cccgaactag tccagtgtgg tggaattctg
      421 cagatatcaa caagtttgta caaaaaagca ggcttcatgg actacaaaga ccatgacggt
      481 gattataaag atcatgacat cgattacaag gatgacgatg acaagcttgc ggccgcgaat
      541 tccgccccca agaaaaagag aaaggtgtgc tccgggcagc ccatccacct gtggcagttc
      601 ctcaaggagt tgctactcaa gccccacagc tatggccgct tcattaggtg gctcaacaag
      661 gagaagggca tcttcaaaat tgaggactca gcccaggtgg cccggctgtg gggcatccgc
      721 aagaaccgtc ccgccatgaa ctacgacaag ctgagccgct ccatccgcca gtattacaag
      781 aagggcatca tccggaagcc agacatctcc cagcgcctcg tctaccagtt cgtgcacccc
      841 atctgagacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
      901 agtctagagg gcccgcggtt cgaataccca tacgacgtcc cagactacgc ttagtaatga
      961 ttaattaaac tagaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagt
     1021 aacccaccca agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     1081 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     1141 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     1201 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     1261 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     1321 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     1381 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     1441 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     1501 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     1561 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
     1621 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     1681 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     1741 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     1801 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     1861 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     1921 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     1981 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     2041 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     2101 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     2161 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     2221 ctggccgttt ttggcttttt tgttagacga agcttggtac cgagctcgga tctccacccc
     2281 gtaccggtcc tgcagtcgaa ttcaccatgt ctagactgga caagagcaaa gtcataaacg
     2341 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
     2401 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     2461 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     2521 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     2581 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     2641 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     2701 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     2761 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     2821 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     2881 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     2941 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
     3001 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg taactaagta
     3061 aggatccgcg gccgcactag aggaattccg cccctctccc ccccccccct ctccctcccc
     3121 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     3181 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     3241 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     3301 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     3361 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     3421 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     3481 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     3541 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     3601 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     3661 ttgaaaaaca cgatgataag cttaccggtc caccatgatt gaacaagatg gattgcacgc
     3721 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     3781 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     3841 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     3901 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     3961 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4021 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4081 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4141 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     4201 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     4261 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     4321 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     4381 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     4441 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgat gtacaagtaa
     4501 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     4561 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     4621 tttagtccct cccaattcga tatcaagctt atcgcgatag atcctaatca acctctggat
     4681 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4741 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4801 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     4861 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     4921 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     4981 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5041 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     5101 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5161 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5221 acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt
     5281 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5341 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     5401 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5461 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5521 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     5581 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt
     5641 taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
     5701 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5761 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5821 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5881 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5941 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6001 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6061 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     6121 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6181 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6241 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6301 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6361 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6421 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6481 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6541 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6601 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6661 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6721 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6781 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6841 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6901 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6961 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7021 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7081 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7141 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7201 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7261 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7321 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7381 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7441 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7501 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7561 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7621 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7681 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct
     7741 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7801 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac
     7861 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7921 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7981 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa
     8041 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8101 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     8161 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
     8221 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     8281 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     8341 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     8401 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     8461 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     8521 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     8581 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     8641 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     8701 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     8761 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8821 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8881 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     8941 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     9001 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     9061 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     9121 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     9181 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     9241 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     9301 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     9361 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     9421 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     9481 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     9541 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     9601 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     9661 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9721 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9781 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9841 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9901 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9961 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    10021 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    10081 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    10141 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    10201 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    10261 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
    10321 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
    10381 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    10441 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggact aggatccttt
    10501 accac
//
