LOCUS       sequence-193617-        9530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GG compatible CDs acceptor NPTII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9530)
  TITLE     Youles SynBio plasmids
REFERENCE   2  (bases 1 to 9530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL86922OD
FEATURES             Location/Qualifiers
     source          1..9530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    36..60
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             369..998
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1427..2500
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2566..2760
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        2967..3071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3072..3866
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4037..4625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4526..4545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4761..4785
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4948..5127
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5158..5212
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5214..6008
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5268..5287)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5878..5897
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6035..6742
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        7754..8099
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8042..8062
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    8255..8276
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8291..8296,8297..8314,8315..8321)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8329..8345
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8334..8356
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8353..8369
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8353..8369
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8365..8688
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8382..8438
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8439..8456)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8439..8455)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8448..8470)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8664..8683)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     terminator      8792..9499
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
       61 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
      121 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga gtcagccgtg
      181 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
      241 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
      301 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
      361 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
      421 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
      481 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
      541 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
      601 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
      661 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
      721 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
      781 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
      841 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
      901 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
      961 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     1021 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     1081 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     1141 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     1201 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     1261 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     1321 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
     1381 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     1441 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     1501 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     1561 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgtc
     1621 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     1681 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     1741 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     1801 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     1861 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     1921 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     1981 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     2041 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     2101 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     2161 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     2221 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     2281 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     2341 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     2401 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     2461 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     2521 gcaaattgcc ctagcagggg aaaaaggtcg aaaaagcttc tttcctgtgg atagcacgta
     2581 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     2641 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     2701 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     2761 actgtctggc cagcgcacag ccgaacagct gcaaaaagcg cccacatcaa ggctccgagt
     2821 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2881 acaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa cagctgcaaa
     2941 aagcgcccac atcaaggctc cgagtgcgcg gaacccctat ttgtttattt ttctaaatac
     3001 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3061 aaaggaagag tatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat
     3121 accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag
     3181 aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg
     3241 tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc
     3301 tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc
     3361 tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg
     3421 agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag
     3481 acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg
     3541 aaaactggga agaggacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga
     3601 cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct
     3661 ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca
     3721 agtggtatga cattgccttc tgcgtccggt cgctcaggga ggatatcggg gaagaacagt
     3781 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt
     3841 atattttact ggatgaattg ttttagctgt cagaccaagt ttactcatat atactttaga
     3901 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     4201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctgct cggatctgtt ggaccggaca
     4681 gtagtcatgg ttgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
     4741 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
     4801 caacttaata acacattgcg gacgttttta atgtactggg gttgaacact ctgtgccgaa
     4861 ttcggatccg gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
     4921 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctgagccgcg
     4981 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa
     5041 gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt
     5101 ccataaattc ccctcggtat ccaattagag tctcatattc actctcctat ttttacaaca
     5161 attaccaaca acaacaaaca acaaacaaca ttacaattac atttacaatt accatggttg
     5221 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5281 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5341 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     5401 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     5461 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     5521 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     5581 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     5641 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     5701 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     5761 atctcgtcgt gactcatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     5821 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     5881 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     5941 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     6001 tcttctgagc gggactctgg ggttcgctag agtcctgctt taatgagata tgcgagacgc
     6061 ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat
     6121 gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat cacccgttac
     6181 tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtaccc tactacttat
     6241 atgtacaata ttaaaatgaa aacaatatat tgtgctgaat aggtttatag cgacatctat
     6301 gatagagcgc cacaataaca aacaattgcg ttttattatt acaaatccaa ttttaaaaaa
     6361 agcggcagaa ccggtcaaac ctaaaagact gattacataa atcttattca aatttcaaaa
     6421 gtgccccagg ggctagtatc tacgacacac cgagcggcga actaataacg ctcactgaag
     6481 ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga gtattggccg
     6541 tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg gccgggtaac
     6601 cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc ccaaatgaag
     6661 tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc gaattttgcg
     6721 acaacatgtc gaggctcagc cgctgcaaga attcaagctt ggaggaattc caatcccaca
     6781 aaaatctgag cttaacagca cagttgctcc tctcagagca gaatcgggta ttcaacaccc
     6841 tcatatcaac tactacgttg tgtataacgg tccacatgcc ggtatatacg atgactgggg
     6901 ttgtacaaag gcggcaacaa acggcgttcc cggagttgca cacaagaaat ttgccactat
     6961 tacagaggca agagcagcag ctgacgcgta cacaacaagt cagcaaacag acaggttgaa
     7021 cttcatcccc aaaggagaag ctcaactcaa gcccaagagc tttgctaagg ccctaacaag
     7081 cccaccaaag caaaaagccc actggctcac gctaggaacc aaaaggccca gcagtgatcc
     7141 agccccaaaa gagatctcct ttgccccgga gattacaatg gacgatttcc tctatcttta
     7201 cgatctagga aggaagttcg aaggtgaagg tgacgacact atgttcacca ctgataatga
     7261 gaaggttagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta gagaggccta
     7321 cgcagcaagt ctcatcaaga cgatctaccc gagtaacaat ctccaggaga tcaaatacct
     7381 tcccaagaag gttaaagatg cagtcaaaag attcaggact aattgcatca agaacacaga
     7441 gaaagacata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     7501 tcataaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ctactgaatc
     7561 taaggccatg catggagtct aagattcaaa tcgaggatct aacagaactc gccgtcaaga
     7621 ctggcgaaca gttcatacag agtcttttac gactcaatga caagaagaaa atcttcgtca
     7681 acatggtgga gcacgacact ctggtctact ccaaaaatgt caaagataca gtctcagaag
     7741 atcaaagggc tattgagact tttcaacaaa ggataatttc gggaaacctc ctcggattcc
     7801 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca
     7861 aatgccatca ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc gacagtggtc
     7921 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt
     7981 ctacaaagca agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc
     8041 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac
     8101 gctcgagtat aagagctcat ttttacaaca attaccaaca acaacaaaca acaaacaaca
     8161 ttacaattac atttacaatt atcgatacaa tgggagaccg cagctggcac gacaggtttc
     8221 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8281 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8341 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg
     8401 tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt
     8461 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8521 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     8581 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     8641 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     8701 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     8761 tacagacaag ctgtgacggt ctcagcttgt cctgctttaa tgagatatgc gagaagccta
     8821 tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg
     8881 tagctcagat ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat
     8941 cgtattttta tgaataatat tctccgttca atttactgat tgtaccctac tacttatatg
     9001 tacaatatta aaatgaaaac aatatattgt gctgaatagg tttatagcga catctatgat
     9061 agagcgccac aataacaaac aattgcgttt tattattaca aatccaattt taaaaaaagc
     9121 ggcagaaccg gtcaaaccta aaagactgat tacataaatc ttattcaaat ttcaaaagtg
     9181 ccccaggggc tagtatctac gacacaccga gcggcgaact aataacgctc actgaaggga
     9241 actccggttc cccgccggcg cgcatgggtg agattccttg aagttgagta ttggccgtcc
     9301 gctctaccga aagttacggg caccattcaa cccggtccag cacggcggcc gggtaaccga
     9361 cttgctgccc cgagaattat gcagcatttt tttggtgtat gtgggcccca aatgaagtgc
     9421 aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca
     9481 acatgtcgag gctcagcagg accgctacta gaggatgcac atgtgaccga
//
