LOCUS       sequence-193624-        9884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express yeast Utp15 with Y2H binding domain fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 9884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pAS2-1-Utp15
FEATURES             Location/Qualifiers
     source          1..9884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1005..1709
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1737..2177
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    2235..2259
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3805..3829)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3859..4046
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3914..3938)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4070..4086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4070..4086)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4083..4105)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4094..4110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4118..4124,4125..4142,4143..4148))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4163..4184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4301..4318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4472..5060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4552..4571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5231..6022,6023..6091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5854..5873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6092..6196)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6223..7565
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7186..7233)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7606..7624
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7662..7684)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7784..7803
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7824..8105
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             8106..8780
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8883..9338)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9020..9041)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9232..9251
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9464..9486
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9478..9495
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9479..9495
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(9529..9776)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     9742..9760
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gatagaaata atacagaagt agatgttgaa ttagattaaa ctgaagatat ataatttatt
       61 ggaaaataca tagagttttt gttgatgcgc ttaagcgatc aattcaacaa caccaccagc
      121 agctctgatt ttttcttcag ccaacttgga gacgaatcta gctttgacga taactggaac
      181 atttgggatt ctacccttac ccaagatctt accgtaaccg gctgccaaag tgtcaataac
      241 tggagcagtt tccttagaag cagatttcaa gtattggtct ctcttgtctt ctgggatcaa
      301 tgtccacaat ttgtccaagt tcaagactgg cttccagaaa tgagcttgtt gcttgtggaa
      361 gtatctcata ccaaccttac cgaaataacc tggatggtat ttatccatgt taattctgtg
      421 gtgatgttga ccaccggcca tacctctacc accggggtgc tttctgtgct taccgatacg
      481 acctttaccg gctgagacgt gacctctgtg ctttctagtc ttagtgaatc tggaaggcat
      541 tcttgattag ttggatgatt gttctgggat ttaatgcaaa aaaatcacta agaaggaaaa
      601 aaatcaacgg agaaagcaaa cgccatctta aatatacggg atacagatga aaggtttgaa
      661 cctatctggg aaaatacgca ttaaacaagc gaaaaactgc gaggaaaatt gtttgcgtct
      721 ctgcgggcta ttcacgcgcc agaggaaaaa taggaaaaat aacaggcatt agaaaaataa
      781 ttttgatttt ggtaatgtgt gggtccctgg tgtacagatg ttacattggt tacagtactc
      841 ttgtttttgc tgtgtttttc gatgaatctc caaaatggtt gttagcacat ggaagagtca
      901 ccgatgctaa gttatctcta tgtaagctac gtggcgtgac ttttgatgaa gccgcacaag
      961 agatacagga ttggcaactg caaatagaat ctggggatct agatatcctt ttgttgtttc
     1021 cgggtgtaca atatggactt cctcttttct ggcaaccaaa cccatacatc gggattccta
     1081 taataccttc gttggtctcc ctaacatgta ggtggcggag gggagatata caatagaaca
     1141 gataccagac aagacataat gggctaaaca agactacacc aattacactg cctcattgat
     1201 ggtggtacat aacgaactaa tactgtagcc ctagacttga tagccatcat catatcgaag
     1261 tttcactacc ctttttccat ttgccatcta ttgaagtaat aataggcgca tgcaacttct
     1321 tttctttttt tttcttttct ctctcccccg ttgttgtctc accatatccg caatgacaaa
     1381 aaaatgatgg aagacactaa aggaaaaaat taacgacaaa gacagcacca acagatgtcg
     1441 ttgttccaga gctgatgagg ggtatctcga agcacacgaa actttttcct tccttcattc
     1501 acgcacacta ctctctaatg agcaacggta tacggccttc cttccagtta cttgaatttg
     1561 aaataaaaaa aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct
     1621 tttgtttcct cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat
     1681 ttcaagctat accaagcata caatcaactc caagcttgaa gcaagcctcc tgaaagatga
     1741 agctactgtc ttctatcgaa caagcatgcg atatttgccg acttaaaaag ctcaagtgct
     1801 ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc
     1861 ccaaaaccaa aaggtctccg ctgactaggg cacatctgac agaagtggaa tcaaggctag
     1921 aaagactgga acagctattt ctactgattt ttcctcgaga agaccttgac atgattttga
     1981 aaatggattc tttacaggat ataaaagcat tgttaacagg attatttgta caagataatg
     2041 tgaataaaga tgccgtcaca gatagattgg cttcagtgga gactgatatg cctctaacat
     2101 tgagacagca tagaataagt gcgacatcat catcggaaga gagtagtaac aaaggtcaaa
     2161 gacagttgac tgtatcgccg gaattgcaat acccagcttt gactcatatg gccatggagg
     2221 ccgaattagc tttcacaagt ttgtacaaaa aagcaggcta caaaatgtcg actgctaggc
     2281 ctagaataat cacttcgaag gctccattat tgcctcaaca gactaccccg gagcagcgtt
     2341 actggcgtca atacacttcc gcacaactgg ttaaagaaca taatagcgtt actcacatat
     2401 cattcaatcc tcaacatcct catgattttg cagtaacttc ctcaaccaga gttcaaatat
     2461 tttcttcaag aactaggcaa gtgatcaaga ccttttcaag atttaaagat gttgtctatt
     2521 ctgcatcctt taggagtgat ggtaaattgc tttgtgccgg tgatgctacc ggtttagtgt
     2581 ctgtatacga cagttataat cccaggacta tccttttatc tataaacgct tccacacatc
     2641 ccacacatgt tacgaaattc catacgcaag ataacaaaat tctggctacg gcgagtgacg
     2701 atagagtgac aagactttgg gatatatcca acgcatatga gccacaatta gagctcaccg
     2761 gagctactga ctatgttcgt accttatcat ttatcccagc agccccacat ttggttgcaa
     2821 caggctcata cgacggtttg atcagattat atgacacaag atctagcgga tccacgccaa
     2881 tttattctct gaatcatgac caacctgttg aaaatgttat tgcagtatct ccaacccaaa
     2941 ttgtttcttg cggtggcaat aatttcaaag tgtgggactt gaccagtaac aagaaattat
     3001 acgaacgtgg caatttcaat aaggctgtga catgcttgga ctatgttgaa aattttgatt
     3061 ctccaatgca atcggcgttg atcgcttctt ccttagatgg tcatgtcaaa gtctttgatc
     3121 ctttagacaa tttccaagtc aaatttggct ggaaattttc gggtcccgta ctaagttgtg
     3181 ctgtctcccc tagtaccgca caagggaata ggcatttagt agcaggttta tcatcaggac
     3241 tactagccat tagaactaaa aagaaagaga aacgttcttc cgataaagaa aacgcacccg
     3301 ccagttttaa taagaatgcg aaaagtaata acttccagag aatgatgcga ggctcggaat
     3361 accaaggtga tcaagagcat ataatccaca atgataaggt aagatctcaa cgtcgtatgc
     3421 gtgcattcga aaggaacata aatcagttta aatggagcga agcactcgac aatgcctttg
     3481 taccaggtat ggccaaagag ttgacactta ctgttcttca agaactacgc aaacgtggca
     3541 aagttcgtgt agcgctatat ggtagagatg aatctacatt agagccgcta ctgaattggt
     3601 gcttaaaagg tattgaagat gtgagatcag catctattgt agcggattgg gtcgccgtgg
     3661 ttttagaatt atatgggaat acgttagaaa gctctccagt tcttcaagaa ttgatgatag
     3721 atttgaaaac taaagttaga catgaaattc ataagtctaa agaagctcaa agaattgaag
     3781 gtatgcttca acttttaacg agtaacccag ctttcttgta caaagtggtg agtcgacctg
     3841 cagccaagct aattccgggc gaatttctta tgatttatga tttttattat taaataagtt
     3901 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     3961 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc
     4021 gctcttattg accacacctc taccggcatg caagcttggc gtaatcatgg tcatagctgt
     4081 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4141 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4201 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4261 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4321 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4381 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4441 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4501 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4561 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4621 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4681 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4741 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4801 acgacttatc gccactggca gcagccacta gtaacaggat tagcagagcg aggtatgtag
     4861 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4921 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4981 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5041 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5101 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5161 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5221 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5281 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5341 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5401 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5461 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5521 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5581 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5641 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5701 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5761 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5821 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5881 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5941 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6001 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6061 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6121 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6181 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt
     6241 cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     6301 tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt
     6361 gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
     6421 tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa
     6481 tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag
     6541 catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt
     6601 ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca
     6661 taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat
     6721 tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt
     6781 gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc
     6841 ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga
     6901 ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa
     6961 acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt
     7021 aggttatata gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt
     7081 gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt
     7141 ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt
     7201 tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc
     7261 gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg
     7321 tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg
     7381 cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt
     7441 atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata
     7501 tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt
     7561 ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     7621 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7681 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     7741 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     7801 gtactgagag tgcaccatag atcaacgaca ttactatata tataatatag gaagcattta
     7861 atagaacagc atcgtaatat atgtgtactt tgcagttatg acgccagatg gcagtagtgg
     7921 aagatattct ttattgaaaa atagcttgtc accttacgta caatcttgat ccggagcttt
     7981 tctttttttg ccgattaaga attaattcgg tcgaaaaaag aaaaggagag ggccaagagg
     8041 gagggcattg gtgactattg agcacgtgag tatacgtgat taagcacaca aaggcagctt
     8101 ggagtatgtc tgttattaat ttcacaggta gttctggtcc attggtgaaa gtttgcggct
     8161 tgcagagcac agaggccgca gaatgtgctc tagattccga tgctgacttg ctgggtatta
     8221 tatgtgtgcc caatagaaag agaacaattg acccggttat tgcaaggaaa atttcaagtc
     8281 ttgtaaaagc atataaaaat agttcaggca ctccgaaata cttggttggc gtgtttcgta
     8341 atcaacctaa ggaggatgtt ttggctctgg tcaatgatta cggcattgat atcgtccaac
     8401 tgcatggaga tgagtcgtgg caagaatacc aagagttcct cggtttgcca gttattaaaa
     8461 gactcgtatt tccaaaagac tgcaacatac tactcagtgc agcttcacag aaacctcatt
     8521 cgtttattcc cttgtttgat tcagaagcag gtgggacagg tgaacttttg gattggaact
     8581 cgatttctga ctgggttgga aggcaagaga gccccgaaag cttacatttt atgttagctg
     8641 gtggactgac gccagaaaat gttggtgatg cgcttagatt aaatggcgtt attggtgttg
     8701 atgtaagcgg aggtgtggag acaaatggtg taaaagactc taacaaaata gcaaatttcg
     8761 tcaaaaatgc taagaaatag gttattactg agtagtattt atttaagtat tgtttgtgca
     8821 cttgccgatc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     8881 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     8941 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     9001 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     9061 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     9121 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     9181 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     9241 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     9301 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc
     9361 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     9421 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     9481 aaaacgacgg ccagtgccaa gctttcgcga gctcgagatc ccgagcttgc aaattaaagc
     9541 cttcgagcgt cccaaaacct tctcaagcaa ggttttcagt ataatgttac atgcgtacac
     9601 gcgtctgtac agaaaaaaaa gaaaaatttg aaatataaat aacgttctta atactaacat
     9661 aactataaaa aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt
     9721 tagagcggat gtggggggag ggcgtgaatg taagcgtgac ataactaatt acatgatatc
     9781 gacaaaggaa aaggggcctg tttactcaca ggcttttttc aagtaggtaa ttaagtcgtt
     9841 tctgtctttt tccttcttca acccaccaaa ggccatcttg gtac
//
