LOCUS       sequence-193625-       12142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express yeast Dhr1 with Y2H binding domain fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pAS2-1-Dhr1
FEATURES             Location/Qualifiers
     source          1..12142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1001..1705
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1733..2173
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    2231..2255
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6063..6087)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      6117..6304
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6172..6196)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(6328..6344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6328..6344)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6341..6363)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6352..6368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6376..6382,6383..6400,6401..6406))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6421..6442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6559..6576)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6730..7318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6810..6829)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7489..8280,8281..8349))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8112..8131
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8350..8454)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8481..9823
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9444..9491)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9864..9882
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9920..9942)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10042..10061
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10082..10363
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             10364..11038
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(11141..11596)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11278..11299)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11490..11509
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11722..11744
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11736..11753
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11737..11753
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(11787..12034)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     12000..12018
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gaaataatac agaagtagat gttgaattag attaaactga agatatataa tttattggaa
       61 aatacataga gtttttgttg atgcgcttaa gcgatcaatt caacaacacc accagcagct
      121 ctgatttttt cttcagccaa cttggagacg aatctagctt tgacgataac tggaacattt
      181 gggattctac ccttacccaa gatcttaccg taaccggctg ccaaagtgtc aataactgga
      241 gcagtttcct tagaagcaga tttcaagtat tggtctctct tgtcttctgg gatcaatgtc
      301 cacaatttgt ccaagttcaa gactggcttc cagaaatgag cttgttgctt gtggaagtat
      361 ctcataccaa ccttaccgaa ataacctgga tggtatttat ccatgttaat tctgtggtga
      421 tgttgaccac cggccatacc tctaccaccg gggtgctttc tgtgcttacc gatacgacct
      481 ttaccggctg agacgtgacc tctgtgcttt ctagtcttag tgaatctgga aggcattctt
      541 gattagttgg atgattgttc tgggatttaa tgcaaaaaaa tcactaagaa ggaaaaaaat
      601 caacggagaa agcaaacgcc atcttaaata tacgggatac agatgaaagg tttgaaccta
      661 tctgggaaaa tacgcattaa acaagcgaaa aactgcgagg aaaattgttt gcgtctctgc
      721 gggctattca cgcgccagag gaaaaatagg aaaaataaca ggcattagaa aaataatttt
      781 gattttggta atgtgtgggt ccctggtgta cagatgttac attggttaca gtactcttgt
      841 ttttgctgtg tttttcgatg aatctccaaa atggttgtta gcacatggaa gagtcaccga
      901 tgctaagtta tctctatgta agctacgtgg cgtgactttt gatgaagccg cacaagagat
      961 acaggattgg caactgcaaa tagaatctgg ggatctagat atccttttgt tgtttccggg
     1021 tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga ttcctataat
     1081 accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat agaacagata
     1141 ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc attgatggtg
     1201 gtacataacg aactaatact gtagccctag acttgatagc catcatcata tcgaagtttc
     1261 actacccttt ttccatttgc catctattga agtaataata ggcgcatgca acttcttttc
     1321 tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa
     1381 tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt
     1441 tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc
     1501 acactactct ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat
     1561 aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg
     1621 tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca
     1681 agctatacca agcatacaat caactccaag cttgaagcaa gcctcctgaa agatgaagct
     1741 actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca agtgctccaa
     1801 agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct actctcccaa
     1861 aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa ggctagaaag
     1921 actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga ttttgaaaat
     1981 ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag ataatgtgaa
     2041 taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc taacattgag
     2101 acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag gtcaaagaca
     2161 gttgactgta tcgccggaat tgcaataccc agctttgact catatggcca tggaggccga
     2221 attagctttc acaagtttgt acaaaaaagc aggctacaaa atgggtactt acagaaaaag
     2281 gtttaatgaa aaagccagat ccggccatat ggccaaatta aaagaactga aaagaattag
     2341 gaataagcag ttcactagac aggatgaaaa cgatgaaaga gtagaaaacc cagattcagc
     2401 tcccgctgag agttctacta cggagccaaa tgccaatgcg gaaattcttg aacctctcac
     2461 tgaagaagaa aagaaaatga agaaaagaaa actacaggag ttatttaccc caaaggaatc
     2521 gaaagtttcg agattgaaga agaaaagact ggataaattt attgaacatc aattaaaaag
     2581 agaggaaaga aagacaatta ttggtaaact tcaagattat aagatagata caagtttgtt
     2641 gacaagctct aaaaggttag gagagggcag gcagactaaa aaggaagaat tcaaagaagc
     2701 actgagttta gaacgtcaag gaagaggaaa tgaacaaaca aatgaaatat tatacgaaga
     2761 atatgaacca aaagtttggg atgagtatgg tgaaggaggg agctctgagg atgatgacgg
     2821 cgaagatgat ttcgaggctt catttggtag tatgccgaaa ccaacggaca atgaggagaa
     2881 gaaatcatca ggttttattg atcatagacc agcaaaattt ggtggatctg gtcttagctt
     2941 tgggtttagc aatataaaag tcataaacaa agaaagcaaa actccgaaga agaaatataa
     3001 ttggagacaa cgagtagaaa tggaagaatt gaagaagcat gggaaagaag atgaaatgga
     3061 tttcgacacg acatctgaag atgatgacga agaggaggat caggaagaag aagacaaaat
     3121 gcatcctagt gaaaacccac tcgaagaagt tgaaagcgca gatagtgaaa ctggctcaga
     3181 aaaatttgac caaaatgatg ttgctaacga attcaaggat tgggccaatc aagaaataaa
     3241 aaaattggaa ggtagagatc aagagctagt tactcccact ttgaacatcg attataaacc
     3301 gataattaga aaggaagatt tagatgatgg tctccaagaa gcttatgttc caataaacga
     3361 gaattccacc cgtaaagcct tctatgttga agttagcaga tcagatgaaa ttcagaaagc
     3421 aagaattcaa ctaccagtat ttggtgaaga acacaaaatt atggaggcta ttcaccataa
     3481 tgatgtagtg attatttgtg gtgaaacggg gtcaggtaag actacacaag tcccacaatt
     3541 tttatatgag gctgggtttg gtgccgaaga ttctccagat tacccaggaa tggtgggtat
     3601 cactcaacca agaagagtgg ccgctgtgtc catggctgaa cgtgtagcta atgaattggg
     3661 ggaccatggt cataaagtag gataccaaat taggtttgac tctaccgcaa aggaggacac
     3721 aaaggtcaag tttatgacag atggtgtctt gctacgagaa atgatgcacg atttcaagtt
     3781 gactaaatat tcctccatta tcatagatga agcacatgaa agaaacatta acactgatat
     3841 tttgatcggt atgcttagcc gttgtgttcg gttaagagct aaactacata aggagaaccc
     3901 gatcgagcac aagaagctaa aactcatcat catgtcagct acattaaggg tatcagattt
     3961 cagcgaaaac aaaacattgt tccccatagc gccccctgtc ttacaggttg atgcaaggca
     4021 gtttccagtc tctattcact ttaatcgtag aactgcattc aactatacag acgaggcttt
     4081 cagaaagact tgcaaaattc accagaaact tcctcctggc gctatattgg tgtttttaac
     4141 cggtcagcag gaaattacac acatggtaaa aaggctaaga aaggaatttc cttttaagaa
     4201 aaactccaag tacaacaaag atttagaaac acccgtttct aaaatgggta tcaattcaaa
     4261 aacaacagat ttggaggctg aggatataga cttcagtgta caagttatcg atcaagataa
     4321 gttcaagagc gcgataagat acgaagaaga tgaaggtaac agtggaaacg gtgaagatga
     4381 ggaagacgaa gaggaagaag ggtttgaaga agtcctaact gaagggcaaa cagctaacga
     4441 tcctttatat gtccttccac tgtattctct attaccaacc aaggaacaaa tgagagtctt
     4501 tcaaaaacct cctcaaggtt ctaggctttg tattgttgca acaaatgttg cagaaacatc
     4561 gttaactata cctggagtta gatatgttgt tgattccggg aggtctaaag agcgtaaata
     4621 taatgaatca aatggcgtac agagttttga agtgggctgg gtaagtaaag ctagtgccaa
     4681 tcaaagaagc ggtagagcag gtcgtactgg accaggccat tgttaccgtt tatattcgtc
     4741 tgctgttttt gaacatgatt tcgaacagtt ctctaaacct gaaatcttaa gaatgccagt
     4801 ggagagcatc gtgttacaaa tgaagagcat ggccattcat aatatcatta attttccatt
     4861 tccaactcct ccagatagag ttgccctttc caaggcaata caattactgc aatatttagg
     4921 tgctcttgat aataaggaga tgattactga agatggtaaa aaaatgagcc tgttcccact
     4981 ttcacccaga ttttccaaaa tgttacttgt ttctgacgaa aaagcctgcc ttccttatat
     5041 tgtagcaatt gttagtgccc tttcggtggg agatcctttt atcaatgaat ttgagttggg
     5101 aataaacgaa atttctagaa aaccaaatcc agatgaaaat ctagatgata agattagaga
     5161 gcacgatgaa tcaacaccag gtatggatcc tgaactgaaa aaggaattgc gcagtaaatt
     5221 ctataaaagc aggagtcagt ttagtaaatt agacaaattt agtgacgtat tccgtttatt
     5281 aagtgttgta agtgcaatgg actacgttcc aaaggaacaa aaagaaatat tcatgaagaa
     5341 aaatttttta agaggcaaac taatggaaga aatagtaaaa ttgaggaagc agttaatgta
     5401 tatcatcaag tccaacactt caaaagaaaa tattgccgta gtaattagga atgaagactt
     5461 gaaaagtgat attccaaacg tcatacaaat aaaactttta aaacaaatga tttgcgctgg
     5521 ttttgtcgac catgttgccg taagagcaga tgttttattt ccagatgatg ctaagataac
     5581 caacagaact tccataatca acattcctta tattcctgtt ttggccacga gaacaccaaa
     5641 tatcgaggac tgctttgtct atatccatcc gacttcaatt ttaaataatt tgggggaaat
     5701 gccacccaaa tacatgctct attattcctt gcaccttggt ggaaataata aaacaaggat
     5761 gaatacttta tgtgacatcg caagcacccc cttagcgaac atagcaagaa agggacttct
     5821 tttgacatac agtaaacctc tgactggtca aggcttgaag accgttaatt tatcacctac
     5881 ggaacggtat tgttatgtgg ttcctagatt tggatccaca gttgacaacg atctcaagat
     5941 tggttgggat ttaaatccta tagcagtgca tcagaagaag caaaaaggcc agtggacagt
     6001 cattaagttt atcaccagaa agggcttcca gaccatcaca ggtgaagaga aagaaaaaaa
     6061 aaacccagct ttcttgtaca aagtggtgag tcgacctgca gccaagctaa ttccgggcga
     6121 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
     6181 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
     6241 tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta
     6301 ccggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6361 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7081 agccactagt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7141 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7261 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7321 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7381 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7501 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7561 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7621 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7681 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7741 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7801 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7861 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7921 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7981 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8041 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8101 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8161 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8221 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8281 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8341 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8461 tccccgaaaa gtgccacctg aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca
     8521 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
     8581 gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa
     8641 atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca
     8701 gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta
     8761 caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct
     8821 cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg
     8881 ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt
     8941 cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc
     9001 cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt
     9061 gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact
     9121 acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta
     9181 ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga
     9241 gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac
     9301 agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat
     9361 tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg
     9421 cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga
     9481 ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc
     9541 acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac
     9601 atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta
     9661 tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc
     9721 gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat
     9781 tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatactaa gaaaccatta
     9841 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     9901 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     9961 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    10021 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatagat
    10081 caacgacatt actatatata taatatagga agcatttaat agaacagcat cgtaatatat
    10141 gtgtactttg cagttatgac gccagatggc agtagtggaa gatattcttt attgaaaaat
    10201 agcttgtcac cttacgtaca atcttgatcc ggagcttttc tttttttgcc gattaagaat
    10261 taattcggtc gaaaaaagaa aaggagaggg ccaagaggga gggcattggt gactattgag
    10321 cacgtgagta tacgtgatta agcacacaaa ggcagcttgg agtatgtctg ttattaattt
    10381 cacaggtagt tctggtccat tggtgaaagt ttgcggcttg cagagcacag aggccgcaga
    10441 atgtgctcta gattccgatg ctgacttgct gggtattata tgtgtgccca atagaaagag
    10501 aacaattgac ccggttattg caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag
    10561 ttcaggcact ccgaaatact tggttggcgt gtttcgtaat caacctaagg aggatgtttt
    10621 ggctctggtc aatgattacg gcattgatat cgtccaactg catggagatg agtcgtggca
    10681 agaataccaa gagttcctcg gtttgccagt tattaaaaga ctcgtatttc caaaagactg
    10741 caacatacta ctcagtgcag cttcacagaa acctcattcg tttattccct tgtttgattc
    10801 agaagcaggt gggacaggtg aacttttgga ttggaactcg atttctgact gggttggaag
    10861 gcaagagagc cccgaaagct tacattttat gttagctggt ggactgacgc cagaaaatgt
    10921 tggtgatgcg cttagattaa atggcgttat tggtgttgat gtaagcggag gtgtggagac
    10981 aaatggtgta aaagactcta acaaaatagc aaatttcgtc aaaaatgcta agaaataggt
    11041 tattactgag tagtatttat ttaagtattg tttgtgcact tgccgatcta tgcggtgtga
    11101 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt
    11161 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
    11221 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
    11281 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
    11341 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
    11401 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
    11461 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
    11521 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
    11581 ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    11641 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    11701 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
    11761 tttcgcgagc tcgagatccc gagcttgcaa attaaagcct tcgagcgtcc caaaaccttc
    11821 tcaagcaagg ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga
    11881 aaaatttgaa atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg
    11941 acctagactt caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg
    12001 cgtgaatgta agcgtgacat aactaattac atgatatcga caaaggaaaa ggggcctgtt
    12061 tactcacagg cttttttcaa gtaggtaatt aagtcgtttc tgtctttttc cttcttcaac
    12121 ccaccaaagg ccatcttggt ac
//
