LOCUS       sequence-193635-        1353 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Full-length human TIG3 in E.coli expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Han BG, Cho JW, Cho YD, Kim SY, Yoon HJ, Song HK, Cheong HK, Jeon 
            YH, Lee DK, Lee S, Lee BI
  TITLE     Expression, purification and biochemical characterization of the 
            N-terminal regions of human TIG3 and HRASLS3 proteins.
  JOURNAL   Protein Expr Purif. 2010 May;71(1):103-7. doi: 
            10.1016/j.pep.2010.01.018. Epub 2010 Jan 25.
  PUBMED    20100577
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.pep.2010.01.018"; journalName: "Protein Expr Purif"; 
            date: "2010-05"; volume: "71"; issue: "1"; pages: "103-7"
COMMENT     Sequence Label: pET28b-hTIG3-1-164
FEATURES             Location/Qualifiers
     source          1..1353
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             35..57
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             76..93
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             103..120
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             625..642
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(695..713)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      709..756
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tttttagagg ggtatcactt ccctctagaa taattttgtt taactttaag aaggagatat
       61 accatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc
      121 catatggctt cgccacacca agagcccaaa cctggagacc tgattgagat tttccgcctt
      181 ggctatgagc actgggccct gtatatagga gatggctacg tgatccatct ggctcctcca
      241 agtgagtacc ccggggctgg ctcctccagt gtcttctcag tcctgagcaa cagtgcagag
      301 gtgaaacggg agcgcctgga agatgtggtg ggaggctgtt gctatcgggt caacaacagc
      361 ttggaccatg agtaccaacc acggcccgtg gaggtgatca tcagttctgc gaaggagatg
      421 gttggtcaga agatgaagta cagtattgtg agcaggaact gtgagcactt tgtcacccag
      481 ctgagatatg gcaagtcccg ctgtaaacag gtggaaaagg ccaaggttga agtcggtgtg
      541 gccacggcgc ttggaatcct ggttgttgct ggatgctctt ttgcgattag gagataccaa
      601 aaaaaagcaa cagcctgact cgagcaccac caccaccacc actgagatcc ggctgctaac
      661 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      721 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      781 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      841 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttcactt
      901 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      961 ggttccgatt tagtgcttta cggcacctcg accccaaaaa cttgattagg gtgatggttc
     1021 acgtaatggg ccatcgccct gaaaaacggt tttcgccctt tgacgttgga ggccacgttc
     1081 tttaaagggg actcttgttc taactggaac accctcaacc ctatcccggc cattcttttg
     1141 atttaaaggg atttggccga ttccgcctat tggttaaaat gagctgatta acataatttt
     1201 acgcgaattt tactaaattt aacgcttcaa tttaaggggc cttttcgggg aaatgggccg
     1261 gaaccccttt tggtatcttc aaaaaaattc aaataaggtc ccgccaagaa ttacttcaaa
     1321 aatcctcagg tccataggga agggatatat tta
//
